Cell numbers and ploidy classes in the normal bladder urothelium in hairless mice. Based on an improved method for dispersion and preparation of cells for flow cytometry.

A simple method for obtaining high-yield single cell suspensions from mouse urothelium is reported. The yield of cells per bladder is about 5 X 10(5), or more than twice that of previously published methods. The method gives a satisfactory coefficient of variation and regularly low levels of debris when used for flow cytometry.