Literature DB >> 28612042

Determination of Adeno-associated Virus Rep DNA Binding Using Fluorescence Anisotropy.

Francisco Zarate-Perez1, Vishaka Santosh1, Martino Bardelli2, Leticia Agundez2, R Michael Linden2, Els Henckaerts2, Carlos R Escalante1.   

Abstract

Quantitative measurement of proteins binding to DNA is a requisite to fully characterize the structural determinants of complex formation necessary to understand the DNA transactions that regulate cellular processes. Here we describe a detailed protocol to measure binding affinity of the adeno-associated virus (AAV) Rep68 protein for the integration site AAVS1 using fluorescent anisotropy. This protocol can be used to measure the binding constants of any DNA binding protein provided the substrate DNA is fluorescently labeled.

Entities:  

Keywords:  Adeno-associated virus; Anisotropy; Fluorescence; Protein-DNA binding; Rep proteins

Year:  2017        PMID： 28612042      PMCID： PMC5466871     

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  10 in total

1.  Residues within the B' motif are critical for DNA binding by the superfamily 3 helicase Rep40 of adeno-associated virus type 2.

Authors:  Miran Yoon-Robarts; Amanda G Blouin; Svenja Bleker; Jürgen A Kleinschmidt; Aneel K Aggarwal; Carlos R Escalante; R Michael Linden
Journal:  J Biol Chem       Date:  2004-09-14       Impact factor: 5.157

2.  Structural Insights into the Assembly of the Adeno-associated Virus Type 2 Rep68 Protein on the Integration Site AAVS1.

Authors:  Faik N Musayev; Francisco Zarate-Perez; Clayton Bishop; John W Burgner; Carlos R Escalante
Journal:  J Biol Chem       Date:  2015-09-14       Impact factor: 5.157

3.  The AAV origin binding protein Rep68 is an ATP-dependent site-specific endonuclease with DNA helicase activity.

Authors:  D S Im; N Muzyczka
Journal:  Cell       Date:  1990-05-04       Impact factor: 41.582

4.  Identification of linear DNA sequences that specifically bind the adeno-associated virus Rep protein.

Authors:  D M McCarty; D J Pereira; I Zolotukhin; X Zhou; J H Ryan; N Muzyczka
Journal:  J Virol       Date:  1994-08       Impact factor: 5.103

5.  DNA structure modulates the oligomerization properties of the AAV initiator protein Rep68.

Authors:  Jorge Mansilla-Soto; Miran Yoon-Robarts; William J Rice; Shailee Arya; Carlos R Escalante; R Michael Linden
Journal:  PLoS Pathog       Date:  2009-07-10       Impact factor: 6.823

6.  Adeno-associated virus (AAV) Rep proteins mediate complex formation between AAV DNA and its integration site in human DNA.

Authors:  M D Weitzman; S R Kyöstiö; R M Kotin; R A Owens
Journal:  Proc Natl Acad Sci U S A       Date:  1994-06-21       Impact factor: 11.205

7.  The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfaces.

Authors:  Alison Burgess Hickman; Donald R Ronning; Zhanita N Perez; Robert M Kotin; Fred Dyda
Journal:  Mol Cell       Date:  2004-02-13       Impact factor: 17.970

8.  A maltose-binding protein/adeno-associated virus Rep68 fusion protein has DNA-RNA helicase and ATPase activities.

Authors:  R S Wonderling; S R Kyöstiö; R A Owens
Journal:  J Virol       Date:  1995-06       Impact factor: 5.103

9.  Crystal structure of the SF3 helicase from adeno-associated virus type 2.

Authors:  J Anson James; Carlos R Escalante; Miran Yoon-Robarts; Thomas A Edwards; R Michael Linden; Aneel K Aggarwal
Journal:  Structure       Date:  2003-08       Impact factor: 5.006

10.  Identification of a Functionally Relevant Adeno-Associated Virus Rep68 Oligomeric Interface.

Authors:  Martino Bardelli; Francisco Zárate-Pérez; Leticia Agúndez; R Michael Linden; Carlos R Escalante; Els Henckaerts
Journal:  J Virol       Date:  2016-07-11       Impact factor: 5.103
  10 in total
  1 in total

1.  Exchange of functional domains between a bacterial conjugative relaxase and the integrase of the human adeno-associated virus.

Authors:  Leticia Agúndez; Francisco Zárate-Pérez; Anita F Meier; Martino Bardelli; Matxalen Llosa; Carlos R Escalante; R Michael Linden; Els Henckaerts
Journal:  PLoS One       Date:  2018-07-17       Impact factor: 3.240
  1 in total

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