Fei Xiong1,2, Ke Hu3,4, Haoli Yu3,4, Lijun Zhou5, Lina Song3,4, Yu Zhang3,4, Xiuhong Shan6, Jianping Liu6, Ning Gu7,8. 1. State Key Laboratory of Bioelectronics, Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. xiongfei@seu.edu.cn. 2. Collaborative Innovation Center of Suzhou Nano-Science and Technology, Suzhou Key Laboratory of Biomaterials and Technologies, Suzhou, 215123, China. xiongfei@seu.edu.cn. 3. State Key Laboratory of Bioelectronics, Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. 4. Collaborative Innovation Center of Suzhou Nano-Science and Technology, Suzhou Key Laboratory of Biomaterials and Technologies, Suzhou, 215123, China. 5. Institute of Pharmaceutics, China Pharmaceutical University, Nanjing, 210096, China. 6. Department of Radiology The Affiliated Renmin Hospital, Jiangsu University, Zhenjiang, China. 7. State Key Laboratory of Bioelectronics, Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. guning@seu.edu.cn. 8. Collaborative Innovation Center of Suzhou Nano-Science and Technology, Suzhou Key Laboratory of Biomaterials and Technologies, Suzhou, 215123, China. guning@seu.edu.cn.
Abstract
PURPOSE: Tumor targeting could greatly promote the performance of magnetic nanomaterials as MRI (Magnetic Resonance Imaging) agent for tumor diagnosis. Herein, we reported a novel magnetic nanoparticle modified with PLA (poly lactic acid)-PEG (polyethylene glycol)-DG (D-glucosamine) as Tumor-targeted MRI Contrast Agent. METHODS: In this work, we took use of the D-glucose passive targeting on tumor cells, combining it on PLA-PEG through amide reaction, and then wrapped the PLA-PEG-DG up to the Fe3O4@OA NPs. The stability and anti phagocytosis of Fe3O4@OA@PLA-PEG-DG was tested in vitro; the MRI efficiency and toxicity was also detected in vivo. RESULTS: These functional magnetic nanoparticles demonstrated good biocompatibility and stability both in vitro and in vivo. Cell experiments showed that Fe3O4@OA@PLA-PEG-DG nanoparticles exist good anti phagocytosis and high targetability. In vivo MRI images showed that the contrast effect of Fe3O4@OA@PLA-PEG-DG nanoparticles prevailed over the commercial non tumor-targeting magnetic nanomaterials MRI agent at a relatively low dose. CONCLUSIONS: The DG can validly enhance the tumor-targetting effect of Fe3O4@OA@PLA-PEG nanoparticle. Maybe MRI agents with DG can hold promise as tumor-targetting development in the future.
PURPOSE:Tumor targeting could greatly promote the performance of magnetic nanomaterials as MRI (Magnetic Resonance Imaging) agent for tumor diagnosis. Herein, we reported a novel magnetic nanoparticle modified with PLA (poly lactic acid)-PEG (polyethylene glycol)-DG (D-glucosamine) as Tumor-targeted MRI Contrast Agent. METHODS: In this work, we took use of the D-glucose passive targeting on tumor cells, combining it on PLA-PEG through amide reaction, and then wrapped the PLA-PEG-DG up to the Fe3O4@OA NPs. The stability and anti phagocytosis of Fe3O4@OA@PLA-PEG-DG was tested in vitro; the MRI efficiency and toxicity was also detected in vivo. RESULTS: These functional magnetic nanoparticles demonstrated good biocompatibility and stability both in vitro and in vivo. Cell experiments showed that Fe3O4@OA@PLA-PEG-DG nanoparticles exist good anti phagocytosis and high targetability. In vivo MRI images showed that the contrast effect of Fe3O4@OA@PLA-PEG-DG nanoparticles prevailed over the commercial non tumor-targeting magnetic nanomaterials MRI agent at a relatively low dose. CONCLUSIONS: The DG can validly enhance the tumor-targetting effect of Fe3O4@OA@PLA-PEG nanoparticle. Maybe MRI agents with DG can hold promise as tumor-targetting development in the future.
Authors: Forrest M Kievit; Freddy Y Wang; Chen Fang; Hyejung Mok; Kui Wang; John R Silber; Richard G Ellenbogen; Miqin Zhang Journal: J Control Release Date: 2011-01-26 Impact factor: 9.776
Authors: Ali S Arbab; Lindsey A Bashaw; Bradley R Miller; Elaine K Jordan; Bobbi K Lewis; Heather Kalish; Joseph A Frank Journal: Radiology Date: 2003-12 Impact factor: 11.105
Authors: Esther Amstad; Stefan Zurcher; Alireza Mashaghi; Joyce Y Wong; Marcus Textor; Erik Reimhult Journal: Small Date: 2009-06 Impact factor: 13.281