Literature DB >> 28603749

Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice.

Julian Christopher Assmann1, Kristin Müller1, Jan Wenzel1, Thomas Walther1, Josefine Brands1, Peter Thornton2, Stuart M Allan3, Markus Schwaninger1.   

Abstract

Brain endothelial cells are the major building block of the blood-brain barrier. To study the role of brain endothelial cells in vitro, the isolation of primary cells is of critical value. Here, we describe a protocol in which vessel fragments are isolated from adult mice. After density centrifugation and mild digestion of the fragments, outgrowing endothelial cells are selected by puromycin treatment and grown to confluence within one week.

Entities:  

Keywords:  Blood-brain barrier; CD31; Claudin-5; Occludin; Primary culture; Tight junctions; VE-cadherin; ZO-1

Year:  2017        PMID： 28603749      PMCID： PMC5464392          DOI： 10.21769/BioProtoc.2294

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  2 in total

1.  Culture of murine brain microvascular endothelial cells that maintain expression and cytoskeletal association of tight junction-associated proteins.

Authors:  Li Song; Joel S Pachter
Journal:  In Vitro Cell Dev Biol Anim       Date:  2003 Jul-Aug       Impact factor: 2.416

2.  Brain endothelial TAK1 and NEMO safeguard the neurovascular unit.

Authors:  Dirk A Ridder; Jan Wenzel; Kristin Müller; Kathrin Töllner; Xin-Kang Tong; Julian C Assmann; Stijn Stroobants; Tobias Weber; Cristina Niturad; Lisanne Fischer; Beate Lembrich; Hartwig Wolburg; Marilyn Grand'Maison; Panayiota Papadopoulos; Eva Korpos; Francois Truchetet; Dirk Rades; Lydia M Sorokin; Marc Schmidt-Supprian; Barry J Bedell; Manolis Pasparakis; Detlef Balschun; Rudi D'Hooge; Wolfgang Löscher; Edith Hamel; Markus Schwaninger
Journal:  J Exp Med       Date:  2015-09-07       Impact factor: 14.307
  2 in total
  15 in total

1.  Telmisartan prevents diet-induced obesity and preserves leptin transport across the blood-brain barrier in high-fat diet-fed mice.

Authors:  Franziska Schuster; Gianna Huber; Ines Stölting; Emily E Wing; Kathrin Saar; Norbert Hübner; William A Banks; Walter Raasch
Journal:  Pflugers Arch       Date:  2018-07-05       Impact factor: 3.657

2.  PTPRD and DCC Are Novel BACE1 Substrates Differentially Expressed in Alzheimer's Disease: A Data Mining and Bioinformatics Study.

Authors:  Hannah A Taylor; Katie J Simmons; Eva M Clavane; Christopher J Trevelyan; Jane M Brown; Lena Przemyłska; Nicole T Watt; Laura C Matthews; Paul J Meakin
Journal:  Int J Mol Sci       Date:  2022-04-20       Impact factor: 6.208

3.  Impaired endothelium-mediated cerebrovascular reactivity promotes anxiety and respiration disorders in mice.

Authors:  Jan Wenzel; Cathrin E Hansen; Carla Bettoni; Miriam A Vogt; Beate Lembrich; Rentsenkhand Natsagdorj; Gianna Huber; Josefine Brands; Kjestine Schmidt; Julian C Assmann; Ines Stölting; Kathrin Saar; Jan Sedlacik; Jens Fiehler; Peter Ludewig; Michael Wegmann; Nina Feller; Marius Richter; Helge Müller-Fielitz; Thomas Walther; Gabriele M König; Evi Kostenis; Walter Raasch; Norbert Hübner; Peter Gass; Stefan Offermanns; Cor de Wit; Carsten A Wagner; Markus Schwaninger
Journal:  Proc Natl Acad Sci U S A       Date:  2020-01-02       Impact factor: 11.205

4.  Endothelial S1P1 Signaling Counteracts Infarct Expansion in Ischemic Stroke.

Authors:  Anja Nitzsche; Marine Poittevin; Ammar Benarab; Philippe Bonnin; Giuseppe Faraco; Hiroki Uchida; Julie Favre; Lidia Garcia-Bonilla; Manuela C L Garcia; Pierre-Louis Léger; Patrice Thérond; Thomas Mathivet; Gwennhael Autret; Véronique Baudrie; Ludovic Couty; Mari Kono; Aline Chevallier; Hira Niazi; Pierre-Louis Tharaux; Jerold Chun; Susan R Schwab; Anne Eichmann; Bertrand Tavitian; Richard L Proia; Christiane Charriaut-Marlangue; Teresa Sanchez; Nathalie Kubis; Daniel Henrion; Costantino Iadecola; Timothy Hla; Eric Camerer
Journal:  Circ Res       Date:  2020-12-02       Impact factor: 17.367

5.  Short regulatory DNA sequences to target brain endothelial cells for gene therapy.

Authors:  Hanna Graßhoff; Helge Müller-Fielitz; Godwin K Dogbevia; Jakob Körbelin; Jacqueline Bannach; Carl Mg Vahldieck; Kristina Kusche-Vihrog; Olaf Jöhren; Oliver J Müller; Ruben Nogueiras; Vincent Prevot; Markus Schwaninger
Journal:  J Cereb Blood Flow Metab       Date:  2021-08-24       Impact factor: 6.960

6.  The LepR-mediated leptin transport across brain barriers controls food reward.

Authors:  Alessandro Di Spiezio; Elvira Sonia Sandin; Riccardo Dore; Helge Müller-Fielitz; Steffen E Storck; Mareike Bernau; Walter Mier; Henrik Oster; Olaf Jöhren; Claus U Pietrzik; Hendrik Lehnert; Markus Schwaninger
Journal:  Mol Metab       Date:  2017-12-07       Impact factor: 7.422

7.  Isolation of endothelial cells, pericytes and astrocytes from mouse brain.

Authors:  Florian Bernard-Patrzynski; Marc-André Lécuyer; Ina Puscas; Imane Boukhatem; Marc Charabati; Lyne Bourbonnière; Charles Ramassamy; Grégoire Leclair; Alexandre Prat; V Gaëlle Roullin
Journal:  PLoS One       Date:  2019-12-18       Impact factor: 3.240

8.  A genome-wide view of the de-differentiation of central nervous system endothelial cells in culture.

Authors:  Mark F Sabbagh; Jeremy Nathans
Journal:  Elife       Date:  2020-01-08       Impact factor: 8.140

9.  Is LRP2 Involved in Leptin Transport over the Blood-Brain Barrier and Development of Obesity?

Authors:  Elvira S Sandin; Julica Folberth; Helge Müller-Fielitz; Claus U Pietrzik; Elisabeth Herold; Thomas E Willnow; Paul T Pfluger; Ruben Nogueiras; Vincent Prevot; Thomas Krey; Markus Schwaninger
Journal:  Int J Mol Sci       Date:  2021-05-08       Impact factor: 5.923

10.  Dual microglia effects on blood brain barrier permeability induced by systemic inflammation.

Authors:  Koichiro Haruwaka; Ako Ikegami; Yoshihisa Tachibana; Nobuhiko Ohno; Hiroyuki Konishi; Akari Hashimoto; Mami Matsumoto; Daisuke Kato; Riho Ono; Hiroshi Kiyama; Andrew J Moorhouse; Junichi Nabekura; Hiroaki Wake
Journal:  Nat Commun       Date:  2019-12-20       Impact factor: 14.919
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