Marina Illiaquer1, Berthe-Marie Imbert-Marcille2, Thierry Guillaume3, Lucie Planche4, Marie Rimbert5, Céline Bressollette-Bodin2, Amandine Le Bourgeois3, Pierre Peterlin3, Alice Garnier3, Claire Le Houerou3, Philippe Moreau3, Mohamad Mohty3, Patrice Chevallier6. 1. Laboratoire de Virologie, CHU Nantes, Nantes, France. 2. Laboratoire de Virologie, CHU Nantes, Nantes, France; Centre de Recherche en Transplantation et Immunologie UMR1064, INSERM, Université de Nantes, Nantes, France; Institut de Transplantation urologie Néophrologie (ITUN), CHU Nantes, Nantes, France. 3. Départment d'Hématologie Clinique, CHU de Nantes, Nantes, France. 4. Département promotion de la recherche clinique, CHU de Nantes, Nantes, France. 5. Laboratoire d'Immunologie, CHU Nantes, Nantes, France. 6. Départment d'Hématologie Clinique, CHU de Nantes, Nantes, France; INSERM, U892, CNRS UMR 6299, Université de Nantes, Nantes, France. Electronic address: patrice.chevallier@chu-nantes.fr.
Abstract
BACKGROUND: Viral infections are well-known complications after allogeneic stem cell transplant (allo-SCT). OBJECTIVES: We compared prospectively incidences of DNAemia and active infections (AI) for five opportunistic viruses (Human Herpesvirus 6 (HHV-6), Epstein-Barr virus (EBV), BK polyomavirus (BKPyV), Cytomegalovirus (CMV) and Adenovirus (ADV)) and kinetics of immune reconstitution (IR) in adults receiving either double umbilical cord blood (dUCB group) or unrelated peripheral blood stem cell (uPBSC group) allo-SCT after a reduced-intensity conditioning (RIC) regimen. STUDY DESIGN: Whole blood samples were collected at transplant, every 15days during the first 3 months and at 4, 5 and 6 months post-transplant. RESULTS: Sixty-five patients were enrolled (uPBSC n=34; dUCB n=31). Incidences of HHV-6 and BKPyV DNAemia were significantly higher for dUCB (97% vs 23.5% and 58% vs 32%, respectively) while EBV DNAemia was more frequently detected in uPBSC (71% vs 26%). The incidence of CMV DNAemia was similar between both groups. ADV AI developed only in dUCB. HHV-6 AI were also higher in dUCB (84% vs 21%). In multivariate analysis, dUCB graft was the only independent factor associated with HHV-6 DNAemia (OR: 19.0; 95%CI: 5.2-69.1; p<0.0001) while EBV DNAemia were significantly associated with uPBSC (OR: 29.9; 95%CI: 5.68-158; p <0.0001). dUCB graft was also the only factor associated with HHV-6 AI. Finally, higher counts and faster recoveries of B lymphocytes (p<0.0001) and monocytes (p=0.0007) were observed in the dUCB group. CONCLUSION: We demonstrate a strong correlation between sources of graft and patterns of viral DNAemia and AI and IR after RIC allo-SCT.
BACKGROUND:Viral infections are well-known complications after allogeneic stem cell transplant (allo-SCT). OBJECTIVES: We compared prospectively incidences of DNAemia and active infections (AI) for five opportunistic viruses (Human Herpesvirus 6 (HHV-6), Epstein-Barr virus (EBV), BK polyomavirus (BKPyV), Cytomegalovirus (CMV) and Adenovirus (ADV)) and kinetics of immune reconstitution (IR) in adults receiving either double umbilical cord blood (dUCB group) or unrelated peripheral blood stem cell (uPBSC group) allo-SCT after a reduced-intensity conditioning (RIC) regimen. STUDY DESIGN: Whole blood samples were collected at transplant, every 15days during the first 3 months and at 4, 5 and 6 months post-transplant. RESULTS: Sixty-five patients were enrolled (uPBSC n=34; dUCB n=31). Incidences of HHV-6 and BKPyV DNAemia were significantly higher for dUCB (97% vs 23.5% and 58% vs 32%, respectively) while EBV DNAemia was more frequently detected in uPBSC (71% vs 26%). The incidence of CMV DNAemia was similar between both groups. ADV AI developed only in dUCB. HHV-6 AI were also higher in dUCB (84% vs 21%). In multivariate analysis, dUCB graft was the only independent factor associated with HHV-6 DNAemia (OR: 19.0; 95%CI: 5.2-69.1; p<0.0001) while EBV DNAemia were significantly associated with uPBSC (OR: 29.9; 95%CI: 5.68-158; p <0.0001). dUCB graft was also the only factor associated with HHV-6 AI. Finally, higher counts and faster recoveries of B lymphocytes (p<0.0001) and monocytes (p=0.0007) were observed in the dUCB group. CONCLUSION: We demonstrate a strong correlation between sources of graft and patterns of viral DNAemia and AI and IR after RIC allo-SCT.
Authors: Ricardo Melendez-Munoz; Rachel Marchalik; Theresa Jerussi; Dimana Dimitrova; Veronique Nussenblatt; Andrea Beri; Khalid Rai; Jennifer S Wilder; A John Barrett; Minoo Battiwalla; Richard W Childs; Courtney D Fitzhugh; Daniel H Fowler; Terry J Fry; Ronald E Gress; Matthew M Hsieh; Sawa Ito; Elizabeth M Kang; Steven Z Pavletic; Nirali N Shah; John F Tisdale; Juan Gea-Banacloche; Christopher G Kanakry; Jennifer A Kanakry Journal: Biol Blood Marrow Transplant Date: 2018-10-18 Impact factor: 5.742
Authors: Harini D de Silva; Rosemary A Ffrench; Maya Korem; Eva Orlowski; David J Curtis; Andrew Spencer; Sharon Avery; Sushrut Patil; Catherine Orla Morrissey Journal: Clin Transl Immunology Date: 2018-10-05
Authors: Anke Janssen; Eline van Diest; Anna Vyborova; Lenneke Schrier; Anke Bruns; Zsolt Sebestyen; Trudy Straetemans; Moniek de Witte; Jürgen Kuball Journal: Viruses Date: 2022-01-10 Impact factor: 5.048