J Rong1,2, C Shan2, S Liu2, H Zheng2, C Liu2, M Liu3,4, F Jin5, L Wang2. 1. Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. 2. Institute of Ageing Research, School of Medicine, Hangzhou Normal University, Hangzhou, China. 3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. 4. University of the Chinese Academy of Sciences, Beijing, China. 5. Key Lab of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China.
Abstract
AIMS: In this study, we investigated the preventive properties of the supernatant of Lactobacillus helveticus NS8-fermented milk (NS8-FS) against UV light-induced skin oxidative damage and hyperpigmentation. METHODS AND RESULTS: NS8-FS exhibited significant radical scavenging activity in tests with ABST+ and DPPH scavenging methods, and as well strongly inhibited 3-morpholinosydnonimine (Sin-1)-induced ROS generation in HaCat keratinocytes. Unexpectedly, NS8-FS was found to inhibit melanin production in B16F10 melanoma cells and to exhibit inhibitory effects both to the enzymatic activity of tyrosinase (TYR) and the expression of proteins required for melanin synthesis. In SKH-1 hairless mice, topical application of NS8-FS alleviated UVB-induced skin photodamage, including the improvement of the appearance of epidermal thickness, transepidennal water loss and lipid peroxidation levels. In the tanning guinea pig model, the whitening effect of NS8-FS was demonstrated using Masson-Fontana staining and TYR staining. Furthermore, NS8-FS was shown to stimulate the nuclear translocation and activation of the Nrf2 protein, along with recovery of antioxidant enzyme activities. CONCLUSION: NS8-FS exhibits the protective capacities against UV light-induced skin oxidative damage and hyperpigmentation. SIGNIFICANCE AND IMPACT OF THE STUDY: Our findings indicate the potential of cell-free fermented products of lactic acid bacteria in topical photoprotection.
AIMS: In this study, we investigated the preventive properties of the supernatant of Lactobacillus helveticus NS8-fermented milk (NS8-FS) against UV light-induced skin oxidative damage and hyperpigmentation. METHODS AND RESULTS:NS8-FS exhibited significant radical scavenging activity in tests with ABST+ and DPPH scavenging methods, and as well strongly inhibited 3-morpholinosydnonimine (Sin-1)-induced ROS generation in HaCat keratinocytes. Unexpectedly, NS8-FS was found to inhibit melanin production in B16F10 melanoma cells and to exhibit inhibitory effects both to the enzymatic activity of tyrosinase (TYR) and the expression of proteins required for melanin synthesis. In SKH-1 hairless mice, topical application of NS8-FS alleviated UVB-induced skin photodamage, including the improvement of the appearance of epidermal thickness, transepidennal water loss and lipid peroxidation levels. In the tanning guinea pig model, the whitening effect of NS8-FS was demonstrated using Masson-Fontana staining and TYR staining. Furthermore, NS8-FS was shown to stimulate the nuclear translocation and activation of the Nrf2 protein, along with recovery of antioxidant enzyme activities. CONCLUSION:NS8-FS exhibits the protective capacities against UV light-induced skin oxidative damage and hyperpigmentation. SIGNIFICANCE AND IMPACT OF THE STUDY: Our findings indicate the potential of cell-free fermented products of lactic acid bacteria in topical photoprotection.
Authors: Miquel Rozas; Astrid Hart de Ruijter; Maria Jose Fabrega; Amine Zorgani; Marc Guell; Bernhard Paetzold; Francois Brillet Journal: Microorganisms Date: 2021-03-18