Cost-effectiveness of triage testing for facility-based systematic screening of tuberculosis among Ugandan adults.

BACKGROUND: Systematic screening is often proposed as a way to improve case finding for tuberculosis (TB), but the cost-effectiveness of specific strategies for systematic screening remains poorly studied.
METHODS: We constructed a Markov-based decision analytic model to analyse the cost-effectiveness of triage testing for TB in Uganda, compared against passive case detection with Xpert MTB/RIF. We assumed a triage algorithm whereby all adults presenting to healthcare centres would be screened for cough, and those with cough of at least 2 weeks would receive the triage test, with positive triage results confirmed by Xpert MTB/RIF. We adopted the perspective of the TB control sector, using a primary outcome of the cost per year of life gained (YLG) over a lifetime time horizon.
RESULTS: Systematic screening in a population with a 5% underlying prevalence of TB was estimated to cost US$610 per YLG (95% uncertainty range US$200-US$1859) with chest X-ray (CXR) (US$5 per test, specificity 0.67), or US$588 (US$221-US$1746) with C reactive protein (CRP) (US$3 per test, specificity 0.59). In addition to the cost and specificity of the triage test, cost-effectiveness was most sensitive to the underlying prevalence of TB, monthly risk of mortality in people with untreated TB and the proportion of patients with TB who would be treated in the absence of systematic screening.
CONCLUSIONS: To optimise the cost-effectiveness of facility-based systematic screening of TB with a triage test, it must be carried out in a high-risk population, or use triage tests that are cheaper or more specific than CXR or CRP.

A key challenge for reducing tuberculosis (TB) mortality lies in detecting the one-third of patients missed by passive case detection. Systematic screening has shown promise to help close this gap.

Previous studies have not considered the cost-effectiveness of scaling up TB case finding by using systematic screening in healthcare facilities.

This model shows that using chest X-ray (CXR) and C reactive protein (CRP) in this way is cost-effective at US$610 and US$588 per year of life gained (YLG), respectively.

Scale-up should be done in high-burden settings, or use triage tools cheaper or more specific than CXR and CRP.

To achieve the WHO goal of a 35% reduction in TB mortality by 2020, substantial improvements in diagnosis must be realised. This analysis suggests that using triage tools in a clinical setting may help achieve this in a cost-effective manner.

Introduction

Tuberculosis (TB) is the leading single-agent cause of infectious mortality worldwide, accounting for an estimated 1.5 million deaths in 2014.1 The WHO and Stop TB Partnership have set ambitious milestones for ending TB, including a 20% reduction in incidence and 35% reduction in mortality by 2020. To achieve these milestones, novel tools and approaches for finding TB cases will be essential.2 3 One key priority in this effort is to diagnose and treat the one-third of all patients with TB who are missed every year by passive case detection.4 5

Systematic screening has been advanced as one way to reach this sizeable undetected population.6 The potential benefits of systematic screening include an increased diagnostic yield, shortened time to diagnosis, cost-effective return on investment and reductions in transmission.7–11 Many methods exist for systematic screening;12 one that has received increasing attention recently is facility-based TB screening, in which all patients presenting to healthcare facilities are actively assessed for TB symptoms regardless of the reason for presentation, and referred for TB testing if symptomatic. However, diagnostic tests such as sputum smear microscopy are poorly sensitive for TB, especially in the context of systematic screening, and more sensitive diagnostic tests (eg, Xpert MTB/RIF, Cepheid, Sunnyvale, California, USA; ‘Xpert’) are too costly to perform on every patient with symptoms.13

One method to improve the cost-effectiveness of facility-based screening for TB is to use a ‘triage’ test.14 Triage tests are rapid, high-sensitivity, lower cost tools used to screen individuals before applying a more costly confirmatory test such as Xpert.7 15 16 The most widely available potential triage test is chest X-ray (CXR), but other biomarker-based triage tests are being developed. C reactive protein (CRP) is a non-specific inflammatory marker that can be measured in minutes from capillary blood and has been suggested in early studies as a TB triage test.17 18 We developed a Markov-based decision analytic model to evaluate the cost-effectiveness of triage testing using CXR or CRP as a means to facilitate facility-based systematic screening for active TB in Uganda.

Methods

Study setting

Uganda is a high-burden country with an estimated TB prevalence of 154 per 100 000, HIV prevalence of 48% among people with incident TB, and prevalence of multidrug-resistant (MDR) TB of 1.4% among new TB cases.1 For this analysis, we considered an upper-level primary health centre (health centre IV) or district-level hospital, in which Xpert testing and chest radiography is available on-site. Although not all health centres and district hospitals have this capacity, triage testing for TB would most likely be implemented first in centres that do. We assumed that initial screening for cough of at least 2 weeks could be performed of individuals presenting to the health centre for any reason, using a two-question screen and at negligible cost. Many algorithms could be implemented to identify people eligible for this initial screening; we did not attempt to specify a particular algorithm, but rather simply assumed that a population of individuals could be identified through facility-based screening for cough, and that this population of individuals with cough would have a given prevalence of TB, which we varied in sensitivity analysis. Our study population therefore consisted of all adults found to have a cough on this initial screen. This population is assumed to include the subset of individuals who, if fully evaluated, would raise sufficient clinical suspicion of active TB that an Xpert test would be ordered under passive case detection, even in the absence of cough screening or triage testing.

Outcomes and economic methods

The primary outcome of this model was the incremental cost-effectiveness ratio (ICER), reported as the incremental cost per year of life gained (YLG), comparing systematic screening with a triage test to passive case finding using Xpert for diagnosis. We adopted the perspective of the TB control sector, using a lifetime time horizon and focusing on costs incurred by the TB control programme. Thus, costs of ongoing antiretroviral therapy (ART) in HIV-infected TB survivors were not incorporated. Estimates of costs and outcomes were drawn from the published literature. Since for TB, YLGs often approximate disability-adjusted life years (DALYs) averted,19 and the cost per YLG is a conservative estimate of the cost per DALY, we used a cost per YLG of less than the per capita gross national income as a threshold for a highly cost-effective intervention.20 All costs were inflated from historical to 2014 US dollars using the US Consumer Price Index.21 22 Capital costs were annualised over the estimated useful life of all capital equipment, and future YLGs and costs were discounted at 3% per year.

Model structure

We developed a Markov-based decision analytic model to simulate triage testing as a strategy for systematic TB screening in Uganda (figure 1). In the baseline scenario (without triage testing), we assumed that only those patients with symptoms sufficient to raise clinical suspicion of active TB would be referred for TB testing, which we assumed would be performed with Xpert MTB/RIF regardless of HIV status. We compared this to a screening scenario in which all individuals presenting to the facility for any reason would be screened for cough of at least 2 weeks, those screening positive would have a triage test performed and those with a positive triage test would be referred for Xpert. In the screening scenario, we assumed that those with sufficient clinical suspicion of active TB would receive Xpert testing, even if the cough screen or triage test result were negative. Thus, systematic screening could increase the number of individuals diagnosed and treated for TB relative to the baseline scenario, but could not decrease that number. Although testing using Xpert for all individuals with TB symptoms is not currently the standard of care in many Ugandan facilities, it is a globally recommended diagnostic algorithm that is practised in other high-burden settings (eg, South Africa). Furthermore, since Xpert should be implemented for people with clinical suspicion of TB before being used for systematic screening, we considered that systematic screening with a triage test would be prioritised in settings where Xpert was already being universally performed for passive TB diagnosis.

Figure 1

Model diagram. All patients with a cough of at least 2 weeks’ duration are first characterised according to active tuberculosis (TB) and HIV status. In the triage testing scenario (upper branch), individuals are then tested with a triage test, with those testing positive on the triage being sent for diagnostic testing with Xpert MTB/RIF; in the standard of care (lower branch), these individuals receive diagnostic testing according to clinical judgement only. Patients with underlying active TB whose diagnosis is missed enter a Markov loop with a 1 month time step, with states as described in the inset.

In this model, individuals with underlying active TB whose diagnosis is missed on initial presentation (either because no TB testing is performed, or because the test result is false-negative and empiric treatment is not initiated) enter a Markov loop with a time step of 1 month. At each month, the individual is assigned a probability of spontaneous resolution, death and returning to the health facility.23 Those who return to the facility enter a similar decision algorithm (screening for cough followed by triage testing in the triage scenario, Xpert testing if active TB is clinically suspected in either scenario), with the conservative assumptions that anyone previously suspected of having active TB will be tested for TB on repeat presentation as well, and that Xpert testing is always positive on a repeat test in someone with active TB. For simplicity, we also assume that all deaths occur instantaneously, without accrual of any YLGs. These assumptions are relaxed in sensitivity analysis. The Markov loop is continued for all individuals with active TB until resolution (spontaneous or through treatment) or death. The model includes the possibilities of empiric treatment in the case of a negative Xpert result (whether true-negative or false-negative), losses to follow-up between ordering of Xpert and initiation of treatment, and losses to follow-up and failures after initiating treatment. Full model parameters are shown in table 1.

Table 1

Model inputs: cohort probabilities, diagnostic parameters and costs

	Value	Range	Reference
Probability parameters
Underlying prevalence of TB among those with a cough of at least 2 weeks' duration	5%	1–10%	24
Prevalence of HIV among those with active TB	48%	40–60%	1
Prevalence of HIV among those with cough but no active TB	7.2%	2–20%	1
Probability of HIV serostatus awareness (among those with HIV/TB)	91%	80–98%	1
Probability of HIV serostatus awareness (among those with HIV and a cough but no active TB)	63%	45–80%	1
Probability that referral for Xpert is completed	85%	65–96%	19
Probability of no treatment after positive Xpert result	0%	0–10%	Assumption
Probability that a patient with TB missed by screening does not return for future diagnosis and treatment	20%	11–30%	25
Probability of empiric treatment among patients with TB testing negative	17%	8–25%	26
Monthly probability of TB spontaneously resolving (HIV+)	0%	0–1%	27
Monthly probability of TB spontaneously resolving (HIV−)	2%	1–6%	23
Probability of TB cure on first-line treatment	77%	65–85%	1
Monthly probability of death from untreated TB	1%	0.5–3%	1
Probability of TB death on first-line treatment	6%	2–15%	1
Probability of loss to follow-up on first-line treatment	17%	*	1
Diagnostic parameters
Sensitivity of clinical diagnosis†	44%	26–63%	26
Sensitivity of CRP (10 mg/L as a positive screen)	98%	80–100%	28 and C Yoon et al. Under review
Sensitivity of CXR (‘any abnormality’ as a positive screen)	98%	72–99%	29
Sensitivity of Xpert	89%	75–95%	30
Specificity of clinical diagnosis†	87%	81–92%	26
Specificity of CRP (10 mg/L as a positive screen)	59%	50–68%	28 and C Yoon et al. Under review
Specificity of CXR (‘any abnormality’ as a positive screen)	67%	50–80%	31
Specificity of Xpert	99%	98–99%	30
Cost parameters
CRP test	US$3	US$2–US$6	Field data
CXR	US$5	US$3–US$10	32
Xpert	US$23.58	US$18–US$28	26
First-line TB therapy (category 1—total)	US$201	US$156–US$243	26
Effectiveness parameters
Discounted life expectancy, HIV-positive	13.75 years	9–19	Assumption
Discounted life expectancy, HIV-negative	17.87 years	13–23	Assumption

*Set equal to (1-probability of cure-probability of death).

†Probability that an individual with active TB will be referred for Xpert testing in the absence of a triage test.

CRP, C reactive protein; CXR, chest X-ray; TB, tuberculosis.

Sensitivity analysis

We performed one-way sensitivity analyses on all model parameters and multiway sensitivity analyses on those parameters to which model results were most sensitive. Ranges for sensitivity analysis were taken from published literature when available; where not available, wide ranges were assumed. We also performed a probabilistic uncertainty analysis by varying all parameters simultaneously across uniform distributions bounded by the corresponding ranges of all model parameters. We report 95% uncertainty ranges (URs) as the 2.5th and 97.5th percentiles of 10 000 model simulations and use cost-effectiveness acceptability curves to describe the proportion of simulations under which each triage test would be cost-effective, as a function of willingness to pay for one YLG.

Results

In the reference scenario, we assumed that 5% of all patients presenting to a healthcare facility with a cough of at least 2 weeks would have TB; thus, for every 10 000 such patients, 500 would have active TB. In the absence of cough screening and triage testing, 166 were projected to be diagnosed and treated on their first presentation (10 of whom died), 285 were diagnosed and treated on subsequent presentation (17 of whom died), 4 resolved spontaneously without treatment, and 18 died of TB without ever being treated. With screening and triage testing (using CXR or CRP), 416 of these 500 patients were diagnosed and treated on initial presentation (27 of whom died), 45 on subsequent presentation (3 of whom died), and 9 were projected to die without treatment. Relative to no triage testing, screening followed by triage with CXR (US$5 per test, specificity 0.67) was estimated to cost US$610 (95% UR US$200–US$1859) per YLG (table 2). This was similar to triage with CRP (US$3 per test, specificity 0.59): US$588 per YLG (US$221–US$1746). Including a fully loaded annual ART cost of US$305 (and assuming lifelong ART for all HIV-positive survivors), the incremental cost-effectiveness of CXR triage rose to US$746 per YLG.33 On probabilistic uncertainty analysis, the probability that screening and triage testing would cost less than the per capita gross national income of Uganda (US$680) per YLG was 0.57 with CXR and 0.59 with CRP (figure 2).

Table 2

Expected costs, days of life gained, and incremental cost-effectiveness of screening for cough followed by triage testing for tuberculosis, per patient presenting with prolonged cough to a healthcare facility in Uganda

Strategy	Average cost (2014 US$)	Incremental cost (2014 US$)	Average years of life lived	Incremental days of life gained	ICER (US$/YLG)	Uncertainty range
Standard of care	US$18.23	–	17.417	–	–	–
CRP	US$24.30	US$6.07	17.427	3.7 days	US$588	US$221–US$1746
CXR	US$24.52	US$6.29	17.427	3.7 days	US$610	US$200–US$1859

Incremental effectiveness and cost-effectiveness for both CRP and CXR are presented relative to the standard of care scenario.

CRP, C reactive protein; CXR, chest X-ray; ICER, incremental cost-effectiveness ratio; YLG, year of life gained.

Figure 2

Cost-effectiveness acceptability curve for tuberculosis triage testing. The curve shows the probability of cost-effectiveness of triage testing with chest X-ray (CXR, blue) or C reactive protein (CRP, red) over 10 000 Monte Carlo simulations. The vertical black line indicates the per capita gross national income of Uganda. YLG, year of life gained.

On one-way sensitivity analysis, the parameters to which the model was most sensitive included the prevalence of active TB among the patient population with a cough; the monthly probability of death (and alternatively of spontaneous resolution) among patients with active, untreated TB; the proportion of patients with TB who would be diagnosed and treated in the absence of triage testing; and the cost and specificity of the triage test (figure 3).

Figure 3

One-way sensitivity analysis on cost-effectiveness of triage testing for tuberculosis (TB) in Uganda. The vertical line represents the base case for chest X-ray (US$610 per year of life gained (YLG)). Ranges for sensitivity analysis are indicated alongside the seven most influential parameters.

Figure 4 shows the settings under which triage testing would be preferred to the standard of care, at a specificity of 0.67 and willingness to pay of US$680 per YLG. If the price of the triage test could be lowered to US$1 or the monthly probability of death from untreated TB was as high as 0.03, triage testing was almost universally preferred. By contrast, in a setting where the prevalence of underlying TB among those screened was 2% or less, triage testing was generally not cost-effective at this willingness-to-pay threshold unless the per-test price could be lowered.

Figure 4

Three-way sensitivity analysis. Blue areas denote combinations of test cost, tuberculosis (TB) prevalence and untreated TB mortality that result in an incremental cost-effectiveness for triage testing, relative to the standard (std) of care, below Uganda's per capita gross national income in 2014 (US$680) for 1 year of life gained. Red areas denote combinations where the standard of care would still be preferred at this willingness-to-pay threshold.

Discussion

This Markov-based decision model illustrates the conditions under which facility-based screening for cough followed by triage testing for TB is likely to be cost-effective in Uganda. Specifically, for a triage test with high sensitivity (98%) to be cost-effective at a willingness to pay of US$680 per YLG in a population where the underlying TB prevalence is 5% and the monthly risk of TB death is 1%, it must cost US$3 (specificity 59%) to US$5 (specificity 67%) per test. If the probability of active TB, TB death or missed TB diagnosis under the standard of care increases, then the corresponding price threshold for the triage test increases accordingly. These results suggest that, for triage testing to be cost-effective in a setting like Uganda, either currently available tests must be implemented in populations with high underlying TB prevalence and high risk of death from untreated TB, or else tests with lower cost and/or higher specificity must be developed and validated.

Our results indicate that implementing existing triage tests may be less preferred, from a cost-effectiveness standpoint, than other interventions such as scale-up of Xpert MTB/RIF for passive diagnosis of TB—which had a more favourable cost-effectiveness profile in Uganda.33 However, the unit cost of triage testing (US$3–US$5) currently remains somewhat high. While CXR and CRP both meet many of the standards laid out in a recently developed target product profile for triage TB tests, that profile listed US$2 as the maximum acceptable unit cost.15 Comparison of CXR and CRP also demonstrates the inherent trade-off between lower cost and higher specificity (which saves money by reducing false-positive screening results). These findings provide strong support for ongoing development and validation of triage tests that can be performed on accessible clinical specimens (eg, capillary blood or urine) at lower cost and/or higher accuracy.

Our sensitivity analysis highlights the importance of the existing standard of care in determining the incremental cost-effectiveness of triage testing for TB. Specifically, triage testing is likely to be most cost-effective where the majority of individuals with TB will not be diagnosed otherwise on clinical grounds (figure 3, red bar for ‘sensitivity of clinical diagnosis’). The cost-effectiveness of triage testing also depends on a screening algorithm that can identify a population at high risk of TB. For example, screening for cough of any duration may identify more individuals with TB than screening for prolonged cough; however, if only 1–2% of individuals with a cough of any duration have TB, then as shown in figure 4, triage-based screening of that population with existing tests is unlikely to be cost-effective.

A previous analysis suggested a substantial reduction in diagnostic costs in Uganda for a theoretical triage test that is 90% sensitive, 75% specific and with a cost of US$5.34 Another recent study similarly suggested that CXR as a triage for Xpert testing in resource-constrained settings could increase throughput while missing few cases of active TB and reducing diagnostic costs.35 Both of these studies conceptualised triage testing as a way to reduce diagnostic costs, relative to a baseline in which Xpert testing was performed on all individuals. Our analysis evaluates a counterbalancing approach, namely of using screening for cough, followed by triage testing, to increase the number of people who could be potentially diagnosed with TB, relative to a baseline in which Xpert testing is performed only among people clinically suspected of having TB. As a result, our findings are naturally less optimistic. Even so, we found that triage testing could be a cost-effective approach to systematic screening if implemented in a high-risk population.

While this study illustrates those conditions in which triage testing can be implemented for systematic screening in cost-effective fashion, several assumptions were necessary. The health facility was assumed to have an infrastructure that is sufficient to support Xpert and CXR/CRP diagnosis, which may limit external validity. Generalisability is also limited in settings with high prevalence of drug resistance, where TB treatment costs are substantially higher and treatment outcomes poorer, making triage testing less cost-effective. If systematic screening were also applied in settings where Xpert was not available on-site, additional costs might be incurred from referring patients or samples, but alternatively, increased effectiveness might be realised from more patients otherwise being missed. In some settings, triage testing may need to be based on symptoms other than cough alone; to the extent that the prevalence of TB and characteristics of the triage test are similar in those populations, our results may still be relevant. We assumed that nearly all patients with a positive triage test would proceed to confirmatory testing on the same visit, also resulting in a potentially inflated effectiveness. We used literature estimates for Xpert's unit cost and sensitivity, both of which may be too high in the setting of systematic screening with declining costs of Xpert implementation; reassuringly, sensitivity analysis around these parameters did not materially influence results. We did not account for transmission from patients whose diagnoses would be missed in the absence of cough screening and triage testing. We also did not incorporate the possibilities that a positive triage test might increase the likelihood of empiric treatment or provide ancillary data (eg, mass on CXR) to suggest an alternative diagnosis. From these perspectives, our results may be biased conservatively (against triage testing). Finally, we compared our estimates of cost-effectiveness only to international benchmarks, not to cost-effectiveness estimates for other TB interventions in Uganda. A complete evaluation would formally compare the cost-effectiveness of triage testing against that of other interventions that might be considered for implementation instead, using the same funds.

In summary, we demonstrate here the conditions under which screening for cough, followed by triage testing, is likely to be cost-effective for evaluation of TB among adults presenting to healthcare facilities in high-burden, low-income settings such as Uganda. If triage tests can be delivered with sensitivity, specificity and unit cost reflecting current best estimates for CXR or CRP, they must be implemented in populations with high underlying TB prevalence (5%) and risk of death from untreated TB (1% per month) to be cost-effective according to commonly used benchmarks. Research to develop and validate triage tests with more favourable characteristics should therefore be prioritised to make such testing cost-effective in lower-risk populations. Ultimately, these findings suggest that triage algorithms can help improve TB case detection, but cheaper and more accurate tests may be necessary in order for broader implementation of TB systematic screening in healthcare facilities to be cost-effective.