A phase Ib study of pembrolizumab plus chemotherapy in patients with advanced cancer (PembroPlus).

BACKGROUND: Pembrolizumab (P) is an anti-PD-1 antibody that blocks the interaction between programmed cell death protein 1 (PD-1) on T-cells and PD-L1 and PD-L2 on tumour cells. A phase Ib trial of P plus chemotherapy was undertaken to evaluate the safety and efficacy.
METHODS: Patients with advanced, metastatic solid tumours were enrolled onto one of six treatment arms. Pembrolizumab was given: with gemcitabine (G), G+docetaxel (D), G+nab-paclitaxel (NP), G+vinorelbine (V) or irinotecan (I) until progression or toxicity, or with liposomal doxorubicin (LD) for up to 15 cycles, progression or toxicity. Safety monitoring and response assessments were conducted.
RESULTS: Forty-nine patients were enrolled and treated. The most common adverse events were transaminitis, cytopenias, rash, diarrhoea, fatigue, nausea and vomiting. Arm 2 was closed due to poor accrual. The recommended phase II dose (RP2D) was determined for Arms 1, 3a, 4, 5 and 6. There were eight partial responses across multiple tumour types.
CONCLUSIONS: Standard dose P can be safely combined with G, G+NP, G+V, I and LD. Efficacy was observed in multiple tumour types and evaluation to determine if response and duration of response are more robust than what would be expected for chemotherapy or immunotherapy alone requires further validation.

In recent years, there has been fervor over the potential promise of immunotherapy for treating advanced solid tumours. Interest was piqued by the first reports of single agent activity of checkpoint inhibitors in low immunogenic cancers such as non-small cell lung cancer (NSCLC) (Herzberg ). Since 2014, there are now three FDA approved inhibitors of programmed cell death protein 1 (PD-1) and PD-1 ligand (PD-L1) with indications across a number of solid tumours, including NSCLC. Yet, for many patients with advanced cancers under those approved indications and many more patients with other types of tumours, the responses and durability of those responses have significant room for improvement.

Cancers may possess multiple modalities to evade immune response including secreting cytokines such as TGF-β and IL-10 or other molecules such as PD-L1 and forming an immune suppressive microenvironment populated with T-regulatory cells (Tregs), macrophages and myeloid-derived suppressor cells (MDSCs) (Duffy and Greten, 2014). By causing apoptotic cell death of cancer cells, chemotherapy can be immunogenic by stimulating anticancer immune effectors directly or mitigating immunosuppressive mechanisms (Zitvogel ). Systemic chemotherapy may stimulate immunosurveillance by antigenicity, immunogenicity or susceptibility (Zitvogel ). Antigenicity is the result of increasing the expression or presentation of tumour-associated antigens on the cell surface of cancer cells. Immunogenicity is causing tumour cells to emit ‘danger’ signals that trigger innate immune responses by operating as adjuvants. Susceptibility is enhancing the likelihood that tumour cells will be recognised and killed by immune effectors.

One means to improve on the efficacy of this approach potentially involves the combination of checkpoint inhibition with agents or tools of different mechanisms of action in the hopes of a deliverance of a windfall of synergism (e.g., chemotherapy, radiotherapy, targeted therapy or other types of immunotherapy). There have been recent clinical data on synergetic effects of cytotoxic chemotherapy given in combination with checkpoint inhibitors (Langer ; Rizvi ). We hypothesise that with sufficient tumour cell kill with the combination of systemic cytotoxic and pembrolizumab (P), a PD-1 inhibitor, the response may be enhanced to achieve long durable complete responses. This phase Ib study was designed to identify the recommended phase II dose (RP2D) for several systemic chemotherapies in combination with P.

Materials and methods

Study design

This Phase Ib, open-label trial included six separate treatment arms for adults with advanced solid tumours and was performed at a single centre in the United States. Enrolment on the phase Ib portion was between 19 December 2014 and 22 July 2015. Prior to initiating any protocol-related activities, signed written informed consent was obtained from each patient. The study protocol, amendments to the protocol and the sample informed consent file were reviewed and approved by the Western Institutional Review Board (WIRB) and the study was registered on clinicaltrials.gov (NCT02331251). The study conformed to Good Clinical Practice guidelines and in accordance with the ethical principles set forth in the Declaration of Helsinki.

P 2 mg kg−1 was administered intravenously over 30 min every 21 days and infused prior to the start of the assigned chemotherapy arm. No dose reductions of P were permitted. The starting dose levels for each treatment arm were as follows:

Arm 1: Gemcitabine 1000 mg m−2 on day 1 and day 8 every 21 days.

Arm 2: Gemcitabine 900 mg m−2 on day 1 and day 8 and docetaxel 75 mg m−2 on day 8 every 21 days.

Arm 3: Gemcitabine 1000 mg m−2 and nab-paclitaxel 125 mg m−2 on day 1 and day 8 every 21 days.

Arm 4: Gemcitabine 1000 mg m−2 and vinorelbine 25 mg m−2 on day 1 and day 8 every 21 days.

Arm 5: Irinotecan 300 mg m−2 on day 1 every 21 days.

Arm 6: Liposomal doxorubicin 30 mg m−2 on day 1 every 21 days (note: the total cumulative dose of liposomal doxorubicin allowed on this protocol is 450 mg m−2 or 15 cycles if there are no dose reductions).

Criteria for inclusion in and exclusion from the study are listed in Section 1 of the Supplementary Section. Patients remained on treatment until disease progression (PD), refusal, withdrawal of consent or occurrence of unacceptable toxicity.

Study end points

The primary objective of the study was to determine the RP2D of chemotherapy in combination with P in subjects with advanced cancer. Secondary objectives included determining (i) the frequency of grade 3 or higher treatment-related adverse events, (ii) the response rate by immune-related response criteria (irRECIST) (Nishino ) and response evaluation criteria in solid tumours (RECIST) 1.1 criteria (Eisenhauer ) and (iii) the overall survival and progression-free survival for enrolled patients.

Assignment of study participants to treatment groups and dose de-escalation modalities

The dose de-escalation scheme (Le Tourneau ) was initiated whereby standard doses for cytotoxic chemotherapy were based or modified to conform with an every 21-day dosing cycle to coincide with standard P dosing at the time of study launch. For example, Arm 3 omitted day 15 gemcitabine and nab-paclitaxel dosing, and on Arm 6 liposomal doxorubicin was based on routine medical oncology practice dosing of 40 mg m−2 on a 28-day schedule and converted to 30 mg m−2 on a 21-day schedule. If on any of the treatment arms, ⩽1 of 3 patients experienced first cycle DLT, up to 3 more patients were enrolled. If ⩾2 or more patients on a dose level experienced first cycle DLTs, the MTD was considered to have been exceeded and 3 patients were treated at the predefined lower dose level. To be declared the RP2D, the dose level being explored would require no more than one of six patients with a DLT.

Toxicity was graded according to the NCI CTCAE version 4.03, with DLT being defined in this study as any event for which the relationship to study treatment could not be definitely excluded. Events that can classify a DLT are provided in Section 2 of the Supplementary Section.

Subjects were replaced if they do not complete the planned dose on cycle 1 day 1 because of an infusion reaction, provided that the infusion reaction was not grade 3 or higher.

Treatment

No more than two intrapatient dose de-escalations were allowed. Initially, dexamethasone premedication was not allowed. However, upon observation of increased nausea, vomiting (despite use of other antiemetic agents), as well as rash and oedema in the extremities due to the systemic chemotherapy, the protocol was amended in September 2015 to require dexamethasone 12 mg intravenous premedication on the days of systemic chemotherapy administration. This decision was also based on observations that safety and efficacy from other ongoing immunotherapy plus chemotherapy trials at the time were not impeded by steroid premedication. Recommended dose modifications in Supplementary Table S1 were only applied to toxicities observed during or after the first and subsequent cycles of treatment.

Removal of participants from treatment or assessment

Patients could continue therapy unless there was PD at any time, they experienced unacceptable toxicity dictating cessation of treatment, there was a change in their medical status (including pregnancy) such that the investigator believed that their safety was compromised or that it was in their best interest to stop treatment, they withdrew consent, they were non-compliant with protocol requirements or were lost to follow-up.

Efficacy assessments

Determination of antitumour efficacy was based on objective tumour assessments performed according to RECIST 1.1 (Eisenhauer ) and irRECIST (Nishino ), and treatment decisions by the investigator were based on these assessments. A clinically stable patient meeting criteria for PD on RECIST 1.1 but with stable disease (SD) or better by irRECIST was permitted to continue on protocol until there was clinical deterioration, significant toxicity or PD by irRECIST.

Safety assessments

Severity of AEs was graded according to NCI CTCAE version 4.03. For each event, the highest severity grade attained was reported. The causality between each AE and study treatment was classified according to the following terms: definitely not related, unlikely related, likely related and definitely related.

Statistical and analytical plans

For the evaluation of the primary end point (i.e., RP2D), all treated patients were considered, except those who had failed to receive a complete first cycle of treatment for reasons other than DLTs. In this case, these patients were replaced with additional patients at the same dose level, in accordance with the protocol. All patients who were evaluable for the primary end point were displayed in the study outputs.

Results

A total of 50 patients were enrolled and 49 patients were dosed on the Phase Ib study. One patient was enrolled but never treated due to an acute GI bleed prior to initiation of treatment. Two patients were unevaluable for DLT assessment and were replaced. At the time of data-cutoff on 1 December 2016, all patients were off study treatment.

The median age at study entry was 55 years and 36 were women (Tables 1 and 2). All but one patient had a KPS performance status of 80% or better at the time of enrolment. The most common cancer types included breast cancer (12 patients), pancreatic adenocarcinoma (PDAC) (11 patients), NSCLC (8 patients), sarcoma (7 patients), small cell lung cancer (SCLC) (5 patients) and ovarian cancer (2 patients). At study entry, all patients were pathologically confirmed to have advanced metastatic disease. Thirty-seven patients (75.6%) including all patients in arms 1, 2, 4 and 5 had been pretreated (having received at least one prior systemic therapy (e.g., chemotherapy, targeted therapy or hormonal therapy) that had been used mostly in the metastatic setting). The number of treatment cycles per patient per treatment arm is provided in Supplementary Table S2.

Table 1

Demographic, baseline and other patient characteristics

	Treated patients (N=49)
Variable	n	%
Demographic characteristics
Age (years)
Median (range)		55 (27–74)
Sex
Male	13	26.5
Female	36	73.5
Performance status (KPS)
100	2	4.1
90	19	37.8
80	27	55.1
70	1	2
Disease characteristics
Primary diagnosis
Breast cancer	12	24.5
Pancreatic cancer	11	22.4
NSCLC	8	16.3
Sarcoma	7	14.3
SCLC	5	10.2
Ovarian cancer	2	4.1
Other	3	6.1
Prior anticancer therapies for metastatic disease
Type of prior therapiesa,b
Systemic only	19	38.8
Surgery+Systemic	2	4.1
Systemic+Radiotherapy	16	32.7
Surgery+Systemic+Radiotherapy	1	2

Abbreviations: KPS=Karnofsky Performance Status; NSCLC=non-small cell lung cancer; SCLC=small cell lung cancer.

Including chemotherapy, targeted therapy or hormone therapy.

A patient may have received more than one type of therapy.

Table 2

Treatment arms and first cycle DLT

Study number	Age at entry	Gender	Cancer type	Prior tx for mets disease	Prior brain mets	KPS at start (%)	Time on Tx (months)	Best response	Survival status	Overall survival (months)
1-0001	31	F	SCC cervix	2 lines CT, XRT	No	90	2.1	PD	Deceased	10.4
1-0002	62	F	TNBC	2 lines CT, XRT	No	80	2.2	PD	Deceased	6.1
1-0003	60	F	ER/PR+BC	XRT	No	100	2.1	PD	Deceased	14.1
1-0004	46	F	ER/PR+BC	1 line CT, 1 line HT	No	80	2	PD	Deceased	8.6
1-0005	74	F	SCLC	1 line CT, 1 line TT	No	80	0.7	PD	Deceased	1.7
1-0006	62	F	ER/PR+BC	2 lines CT, 1 line HT, 1 line TT, XRT	No	90	0.7	PD	Deceased	9
2-0001	61	M	NSCLC-adenocarcinoma EGFR, KRAS, ALK, ROS1 wt	2 lines CT, XRT	No	80	4	SD	Deceased	11.6
3-0001	63	M	PDAC	SX	No	80	9.1	SD	Deceased	10.3
3-0002	47	F	PDAC	1 line CT	No	90	5	SD	Deceased	14.1
3-0003	55	F	PDAC	1 line CT	No	80	4.9	SD	Deceased	8
3-0004	55	F	PDAC	None	No	80	15.3	PR	Alive	17.5
3-0005	57	M	PDAC	1 line CT	No	80	0.9	NE	Deceased	3.3
3-0007	62	M	PDAC	SX	No	90	10.8	PR	Deceased	15
3-0008	63	F	PDAC	None	No	100	4.4	SD	Alive	11.3
3-0009	57	F	PDAC	None	No	80	4.9	SD	Deceased	6.7
3-0010	61	F	PDAC	None	No	80	4.9	SD	Deceased	7.1
3-0011	52	F	PDAC	1 line CT, XRT	No	80	2	PD	Deceased	2.9
3-0013	46	F	PDAC	1 line CT	No	80	2.1	PD	Deceased	4.1
4-0001	64	F	NSCLC-adenocarcinoma EGFR, KRAS, ALK, ROS1 wt	1 line CT, 1 line another PD-1 inhibitor	No	80	3	SD	Deceased	5.7
4-0002	56	F	TNBC	1 line CT	No	80	1.6	PD	Deceased	12.8
4-0003	67	F	Uterine leiomyosarcoma	4 lines CT, 2 lines TT, XRT	No	90	0.9	NE	Deceased	10.3
4-0004	32	F	ER/PR+BC	4 lines CT, XRT	No	70	2.3	PD	Deceased	3.7
4-0005	49	M	Fibromyxoid sarcoma	3 lines CT, 1 line TT, XRT, SX	No	90	2.1	PD	Alive	19.2
4-0006	55	F	ER/PR/HER2+BC	1 line CT, 1 line HT, 2 lines TT, SX	No	80	5.6	PR	Alive	11.4
4-0007	39	F	Synovial sarcoma	1 line CT	No	90	2.1	PD	Alive	17.8
4-0008	55	F	ER/PR+BC	1 line CT, 2 lines HT, 1 line TT, SX	No	80	4.9	SD	Deceased	14.9
4-0009	27	F	Synovial sarcoma	1 line CT, XRT	No	90	2.6	PD	Alive	13.3
4-0010	58	F	ER/PR+BC	1 line CT, 3 lines HT, 1 line TT, XRT	No	90	2.1	PD	Deceased	5.1
4-0011	46	F	ER/PR+BC	3 lines CT, 2 lines HT	No	90	3	PD	Alive	8.4
4-0012	61	F	ER/PR+BC	2 lines CT	No	90	6.5	SD	Alive	7.9
5-0001	57	M	SCLC	1 line CT, XRT	No	90	10.6	PR	Deceased	23.3
5-0002	44	F	SCLC	1 line CT	No	90	10.5	PR	Alive	23.4
5-0003	33	F	NSCLC-adenocarcinoma EGFR+	1 line CT, 2 lines TT, 1 line another PD-1 inhibitor, XRT	Yes	80	2.1	PD	Deceased	3.7
5-0004	48	F	NSCLC-adenocarcinoma KRAS+	2 lines CT	No	80	2	PD	Deceased	2.4
5-0005	45	M	NSCLC-adenocarcinoma KRAS+	2 lines CT	No	80	0.2	PD	Deceased	1.6
5-0006	60	M	NSCLC-adenocarcinoma EGFR+	4 lines CT, 2 lines TT, XRT	Yes	80	4.4	SD	Deceased	6.5
5-0007	59	M	NSCLC-adenocarcinoma KRAS+	2 lines CT, XRT	Yes	90	11.8	PR	Alive	17.5
5-0008	51	F	SCLC	1 line CT, XRT	No	90	1.7	PD	Deceased	2.9
5-0009	61	F	NSCLC-adenocarcinoma EGFR, KRAS, ALK, ROS1 wt	1 line CT	No	80	0.7	NE	Deceased	6.1
5-0010	60	M	SCLC	1 line CT, XRT	No	90	13.7	PR	Alive	13.8
5-0011	49	M	Colorectal cancer-MSI+	1 line CT	No	80	2.7	PD	Deceased	9.5
5-0012	41	M	Esophageal-HER2 negative	1 line CT	No	80	15.4	PD	Deceased	15.4
6-0001	55	F	Endometrial	None	No	80	0	NE	Deceased	7.3
6-0002	50	F	Liposarcoma	None	No	80	6.9	SD	Alive	23.1
6-0003	52	M	Malignant fibrious histiocytoma (sarcoma)	SX	No	80	2.6	PD	Deceased	13.1
6-0004	59	F	OC	4 lines CT	No	90	15.5	SD	Alive	22.4
6-0005	32	F	Clear cell sarcoma	SX	No	90	4.7	PD	Deceased	6.9
6-0006	56	F	OC	1 line CT	No	90	10.5	PR	Alive	17.3
6-0007	58	F	ER/PR+BC	1 line CT, 1 line HT, 1 line TT, XRT	Yes	80	2.1	PD	Deceased	4.1

Abbreviations: ALK=anaplastic lymphoma kinase; BC=breast cancer; CT=chemotherapy; EGFR=epidermal growth factor receptor; ER/PR=oestrogen receptor/progesterone receptor; F=female; HT=hormonal therapy; KPS=Karnofsky Performance Status; KRAS=Kirsten rat sarcoma viral oncogene; M=male; mets=metastatic; MSI=microsatellite instability; NE=not evaluable; NSCLC=non-small cell lung cancer; OC=ovarian carcinoma; PD=disease progression; PDAC=pancreatic adenocarcinoma; PR=partial response; ROS1=ROS proto-oncogene 1; SCC=squamous cell carcinoma; SCLC=small cell lung cancer; SD=stable disease; SX=surgery; TNBC=triple negative breast cancer; TT=targeted therapy; wt=wild type; XRT=radiotherapy; Tx=treatment.

Dose de-escalation by arm and first cycle DLTs

In Arm 1, there was one DLT (received less than 25% planned dose due to grade 4 neutropenia), and RP2D is gemcitabine 1000 mg m−2 days 1 and 8 every 21 days with P on day 1. Arm 2 enrolled one patient and was closed for futility after observing that several prescreened patients would not be eligible for this treatment arm and it would not accrue in an adequate time frame. Arm 3 initially enrolled treatment naïve and previously treated PDAC patients. There were two DLTs (grade 3 thrombocytopenia) observed in the first five patients. Upon further review, these DLTs were seen only in previously treated PDAC patients. The protocol was amended to split this arm into 3a (treatment naïve PDAC) and 3b (previously treated PDAC), where Arm 3b was dose reduced to gemcitabine 800 mg m−2 and nab-paclitaxel 100 mg m−2 on days 1 and 8 every 21 days with P on day 1. The RP2D for Arm 3a is gemcitabine 1000 mg m−2 and nab-paclitaxel 125 mg m−2 on days 1 and 8 every 21 days with P on day 1. On dose level 1 on Arm 4, there were two DLTs in six patients (received less than 25% planned dose due to grade 3 and grade 4 neutropenia, respectively). On dose level −1, there was one DLT in six patients (grade 3 thrombocytopenia) and the RP2D for Arm 4 is gemcitabine 800 mg m−2 and vinorelbine 20 mg m−2 on days 1 and 8 every 21 days with P on day 1. On dose level 1 on Arm 5 there were two DLTs in five patients (grade 3 fatigue and grade 3 nausea, vomiting, and diarrhoea). On dose level −1, one patient withdrew consent and was not evaluable for DLT. At this dose level, there was one DLT in six patients (grade 3 rash and papilloedema), and the RP2D for Arm 5 is irinotecan 250 mg m−2 with P on day 1 every 21 days. Arm 6 had one patient that developed a grade 2 infusion reaction within the first 2 min of liposomal doxorubicin infusion and because of safety concerns with drug re-challenging, she was removed from the study and replaced. Going forward, premedication with diphenhydramine was mandatory on Arm 6 and there were no DLTs in the subsequent six patients. The RP2D for Arm 6 is liposomal doxorubicin 30 mg m−2 with P on day 1 every 21 days (Table 3).

Table 3

Treatment arms and first cycle DLT

Treatment arm	Number of treated patients	No of patients with cycle 1 DLT	DLT
1	6	1	Grade 4 neutropenia leading to ⩾25% missed planned dose of treatment
2	1	0	None
3	11	2	Two with grade 3 thrombocytopenia leading to ⩾25% missed planned dose of treatment (both patients were previously treated with systemic chemotherapy for advanced disease)
4	12	3	Dose level 1: Grade 3 and grade 4 neutropenia, respectively, leading to ⩾25% missed planned dose of treatment
			Dose level −1: Grade 3 thrombocytopenia leading to ⩾25% missed planned dose of treatment
5	12	3	Dose level 1: Grade 3 fatigue and grade 3 nausea, vomiting and diarrhoea, respectively.
			Dose level −1: Grade 3 rash and papilloedema
6	7	0	None

Abbreviation: DLT=dose limiting toxicity.

Safety results by treatment arm

All (100%) receiving study treatment experienced at least one treatment-emergent AE (TEAE), with 28 patients (57.1%) experiencing TEAEs of grade 3–4 (Table 4). Once dexamethasone premedication was introduced to all subsequent patients (affecting Arms 3–5), the incidence of gastrointestinal AEs (e.g., nausea, vomiting) and oedema in the extremities and rash decreased.

Table 4

Treatment emergent adverse events (>20% all grades, >10% for grades 3–4)

		Arm 1 (N=6)		Arm 2 (N=1)		Arm 3a (N=9)		Arm 3b (N=2)		Arm 4 (N=12)		Arm 5 (n=12)		Arm 6 (n=7)
Preferred term	CTC grade	n	%	n	%	n	%	n	%	n	%	n	%	n	%
Any term	1–4	6	100	1	100	9	100	2	200	12	100	12	100	7	100
	3–4	5	83.3	1	100	6	66.7	1	50	8	75	3	25	4	57.1
Thrombocytopenia	1–4	2	33.3	1	100	4	44.4	1	50	7	58.3
	3–4					3	33.3
Neutropenia	1–4	3	50	1	100	3	33.3			8	75	3	25
	3–4	3	50	1	100	2	22.2			4	33.3
Anaemia NOS	1–4	2	33.3	1	100	8	88.9	1	50	8	75			2	28.6
	3–4									2	16.7
AST elevation	1–4	5	83.3			4	44.4			7	58.3
	3–4	2	33.3			1	11.1			2	16.7
ALT elevation	1–4	4	66.7			6	66.7			7	58.3
	3–4	2	33.3			1	11.1
Fatigue	1–4	2	33.3	1	100	5	55.5			4	33.3	5	41.7	2	28.6
	3–4	1	16.7
Hyponatraemia	1–4			1	100	3	33.3
	3–4					2	22.2
White blood cell count decreased	1–4	4	66.7	1	100					9	75
	3–4	3	50	1	100					3	25
Thrombolic event	1–4					3	33.3
	3–4					1	11.1
ALK increased	1–4							2	100	3	25
	3–4							1	50
Diarrhoea	1–4					3	33.3					9	75	3	42.9
	3–4													1	14.3
Pruritus	1–4					2	22.2			3	25			2	28.6
	3–4													1	14.3
Palmar-plantar erythrodysesthesia	1–4													2	28.6
	3–4													1	14.3
Peripheral sensory neuropathy	1–4					4	44.4
	3–4					1	11.1
Nausea	1–2					2	22.2	1	50	5	41.7	8	75	2	28.6
Vomiting	1–2	3	50			3	33.3	1	50	3	25	4	33.3	2	28.6
Rash NOS	1–2	2	33.3	1	100	4	44.4	1	50			3	25	5	71.4
Constipation	1–2					1	11.1	1	50	4	33.3
Weight loss	1–2			1	100	2	22.2
Dysgeusia	1–2			1	100	2	22.2
Oedema in limbs	1–2			1	100	2	22.2
Tracheal hemorrhage	1–2			1	100
Haematoma	1–2			1	100
Pain in extremities	1–2			1	100	3	33.3	1	50	4	33.3	4	33.3
Hypertension	1–2			1	100
Pleural effusion	1–2			1	100
Mucositis oral	1–2					3	33.3							3	42.9
Hypoalbuminaemia	1–2					3	33.3			4	33.3
Dehydration	1–2					3	33.3
Fever	1–2					5	55.5
Insomnia	1–2					4	44.4
Cough	1–2							1	50
Epistaxis	1–2					2	22.2
Hyperphosphataemia	1–2									3	25			2	28.6
Headache	1–2									4	33.3
Anorexia	1–2											3	25
Skin infection	1–2													2	28.6
Rectal and vaginal hemorrhage	1–2							1	50
Hot flashes	1–2					2	22.2
Chills	1–2					2	22.2
Abdominal pain	1–2							1	50
Hypokalaemia	1–2					2	22.2
Hypoxia	3–4									2	16.7
Pneumonia NOS	3–4					1	11.1			2	16.7
Syncope	3–4			1	100
Dyspnoea	3–4					1	11.1
Capillary leak syndrome	3–4					1	11.1
Device infection	3–4					1	11.1

Abbreviations: ALK=alkaline phosphatase; AST, aspartate aminotransferase; NOS=not otherwise specified.

Immune-related adverse events (irAEs) (likely or definitely related) were reported in 50%, 100%, 77.8%, 0%, 33.3%, 33.3% and 57.1% of patients on Arms 1, 2, 3a, 3b, 4, 5 and 6, respectively. Of these, two irAEs led to a dose reduction (both DLTs, one in Arm 4 for grade 3 hypoxia with grade 2 nausea and vomiting and the other in Arm 5 for grade 3 rash and papilloedema). Patient level TEAEs are provided in Supplementary Table S2.

After mandatory premedication with dexamethasone was initiated (see Supplementary Table S2), the frequency of grade 3/4 events appears to have decreased. The average number of grade 3/4 events per patient that enrolled prior to this amendment was 1.1 vs 0.75 grade 3/4 events per patient, respectively. The incidence of likely or definitely related irAEs for patients was also higher prior to the amendment at 20 of 37 (54.1%) compared with 4 of 12 (33.3%).

Two patients died during the study (i.e., within 30 days of coming off study) due to PD (one case each of PDAC and NSCLC, respectively), but these deaths were deemed not to be related to the study medication.

Efficacy results by treatment arm

Forty-five of 49 patients (92%) treated on the study were evaluable for efficacy. On Arm 1, the best response was PD. On Arm 2, the best response was SD. On Arm 3a, the best response was partial response (PR) for two patients and SD for six patients. On Arm 3b, the best response was PD. On Arm 4, the best response was PR for one patient, SD for three patients, and PD for 7 patients. On Arm 5, the best response was PR for four patients, SD for one patient and PD for six patients. On Arm 6, the best response was PR for 1 patient, SD for two patients, and PD for three patients (Table 5). Representative responders for Arms 3a, 4, 5 and 6 are displayed in Supplementary Figures S1–S4.

Table 5

Best tumour response

	Treatment arm
Best tumour response by irRECIST and RECIST 1.1	Arm 1 (N=6)	Arm 2 (N=1)	Arm 3a (N=9)	Arm 3b (N=2)	Arm 4 (N=12)	Arm 5 (N=12)	Arm 6 (N=7)
Partial response	0	0	2	0	1	4	1
Stable disease	0	1	6	0	3	1	2
Progressive disease	6	0	0	2	7	6	3
Not evaluable	0	0	1	0	1	1	1

Discussion

In 2016, nearly 600 000 individuals diagnosed with cancer will die from their disease (Cancer Facts & Figures 2016 | American Cancer Society). While some may have long-term disease-free intervals, for most individuals who are diagnosed with metastatic disease, the survival rate is less than 5 years. For primary cancers of the lung, connective tissue or pancreas, few individuals will live 2 years with metastatic disease. Patients with metastatic disease are usually treated with systemic chemotherapy, with the intent of prolonging survival and palliate symptoms (e.g., pain, weight loss and decreased performance status). For the most common advanced stage cancer, there are consensus guideline first- and/or second-line systemic treatment recommendations. Year after year, randomised trials are designed and launched to try and improve on median overall survival outcomes. In oncology, the success rate from phase I to FDA approval is a dismal 11% (Hay, 2011). Even with the successful phase III clinical trials, the improvement in overall survival is modest, increasing the median by weeks to several months. For common non-haematologic cancers (and many rare cancers), there are no design strategies that are primarily seeking to attain complete (and hopefully durable) responses.

There have been promising results with checkpoint inhibitors across multiple tumours, including in melanoma, renal cell carcinoma and NSCLC (Topalian ; Robert ). There is now accumulating data on the presence of PD-L1 expression across a number of tumour types, including SCLC, PDAC and sarcoma (Bigelow ; Kim ; Yu ). While PD-L1 and/or mutational tumour burden appear to be useful for identifying those most likely to benefit from single-agent checkpoint inhibition, when combination therapy is considered this biomarker does not appear to have a definitive role (Topalian ; Wolchok ; Le ). Additionally, the functional state of the host immune system and/or its interaction with microbiota can have an impact on the therapeutic efficacy of systemic treatment (Sivan ; Vetizou ).

The present study is one of the first reported multi-arm systemic chemotherapy in combination with PD-1 inhibitors across diverse advanced solid tumours. Several systemic chemotherapy agents have been implicated in having immunotherapeutic-enhancing properties. For the agents evaluated in this study, we briefly outline the reported effects of gemcitabine, docetaxel, paclitaxel, vinorelbine, irinotecan and doxorubicin (Galluzzi ; Duffy and Greten, 2014). Gemcitabine can increase class I HLA expression, enhance tumour antigen cross-presentation and selectively kill MDSCs. Docetaxel can decrease MDSCs. Paclitaxel can stimulate antigen-presenting dendritic cells and increase tumour cell permeability to granzyme B. Vinorelbine can facilitate the bystander death of immune cells. Irinotecan can decrease MDSC and Tregs. Doxorubicin can induce immunogenic cell death, increase tumour cell permeability to granzyme B and stimulate antigen presentation dendritic cells.

Overall, 50 patients with advanced/metastatic solid tumours were enrolled and 47 were evaluable for the primary endpoint. Each completed treatment arm has a RP2D. Arm 3b is unlikely to complete accrual for RP2D. Main toxicities observed were transaminitis, cytopenias, rash, diarrhoea, fatigue, nausea and vomiting. There do not appear to be a signal for increased immune-related AEs, particularly once dexamethasone premedication was administered on the days of systemic chemotherapy infusion. There were multiple responses observed and a few appear to be supra-normal and may be a signal of potential synergy. The phase II portions of the arms with a RP2D are ongoing and subsequent future reporting of those results are planned.

There are ongoing studies involving a variety of immunotherapy plus targeted or chemotherapy agents now across a number of different cancer types and it remains to be seen which of these combinations will be true game changers and deliver long lasting responses with manageable or minimal toxicity. In conclusion, this study was successful in identifying the RP2D of multiple systemic chemotherapies in combination with P and in characterising the safety profile of these combinations on a 21-day treatment cycle.