Tao Zhang1, Fuying Tian2, Ran Huo3, Aifa Tang2, Yong Zeng1, Yong-Gang Duan2,3. 1. Fertility Center, Shenzhen-Zhongshan Urology Hospital, Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen-Zhongshan Institute for Reproduction and Genetics, Shenzhen, China. 2. Centre of Reproductive Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China. 3. Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong, Shenzhen Hospital, Shenzhen, China.
Abstract
PROBLEM: The presence of dendritic cells (DCs) and associated cytokines in follicular fluid (FF) from patients with polycystic ovary syndrome (PCOS) remains unknown. METHODS OF STUDY: FF was collected from PCOS patients and patients with severe male factor infertility (control) at the day of transvaginal oocyte retrieval. Phenotypes of DC were detected by flow cytometry, and TNF-α, IL-6, IL-10, and IL-23 were assessed by ELISA. RESULTS: A significant decrease in the percentage of DC was found in patients with PCOS (16.22±5.5%) compared with control (21.27±5.5%, P<.01). E2 on the day of hCG administration was correlated positively with the mean fluorescence intensity of HLA-DR (r=.75, P<.01) and reversely correlated with the concentration of TNF-α in FF (r=-.69, P<.01). The level of TNF-α, IL-6, and IL-10 increased significantly but IL-23 decreased in FF from patients with PCOS. CONCLUSION: The decrease of DC and disturbance of associated cytokines in FF from PCOS patients indicates a disorder of immunological microenvironment of the ovarian follicle, which might be involved in the dysfunction of folliculogenesis.
PROBLEM: The presence of dendritic cells (DCs) and associated cytokines in follicular fluid (FF) from patients with polycystic ovary syndrome (PCOS) remains unknown. METHODS OF STUDY: FF was collected from PCOSpatients and patients with severe male factor infertility (control) at the day of transvaginal oocyte retrieval. Phenotypes of DC were detected by flow cytometry, and TNF-α, IL-6, IL-10, and IL-23 were assessed by ELISA. RESULTS: A significant decrease in the percentage of DC was found in patients with PCOS (16.22±5.5%) compared with control (21.27±5.5%, P<.01). E2 on the day of hCG administration was correlated positively with the mean fluorescence intensity of HLA-DR (r=.75, P<.01) and reversely correlated with the concentration of TNF-α in FF (r=-.69, P<.01). The level of TNF-α, IL-6, and IL-10 increased significantly but IL-23 decreased in FF from patients with PCOS. CONCLUSION: The decrease of DC and disturbance of associated cytokines in FF from PCOSpatients indicates a disorder of immunological microenvironment of the ovarian follicle, which might be involved in the dysfunction of folliculogenesis.
Authors: Sai-Jiao Li; Hsun-Ming Chang; Jiamin Xie; Jeremy H Wang; Jing Yang; Peter C K Leung Journal: Biol Reprod Date: 2021-11-15 Impact factor: 4.161