Literature DB >> 2858208

Separation of the cytolytic and mosquitocidal proteins of Bacillus thuringiensis subsp. israelensis.

J M Hurley, S G Lee, R E Andrews, M J Klowden, L A Bulla.   

Abstract

The cytolytic and mosquitocidal proteins of Bacillus thuringiensis subsp. israelensis were isolated from parasporal crystals and subsequently separated from each other. The proteins were separated by gel filtration chromatography and their molecular weights were estimated by both gel filtration chromatography and SDS-polyacrylamide gel electrophoresis. The apparent molecular weights of the mosquitocidal protein and the cytolytic protein were estimated to be 65,000 daltons and 28,000 daltons, respectively.

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Year:  1985        PMID: 2858208     DOI: 10.1016/0006-291x(85)90279-7

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN: 0006-291X            Impact factor:   3.575


  27 in total

1.  Cloning and expression of two homologous genes of Bacillus thuringiensis subsp. israelensis which encode 130-kilodalton mosquitocidal proteins.

Authors:  E S Ward; D J Ellar
Journal:  J Bacteriol       Date:  1988-02       Impact factor: 3.490

2.  The glycoprotein toxin of Bacillus thuringiensis subsp. israelensis indicates a lectinlike receptor in the larval mosquito gut.

Authors:  G Muthukumar; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1987-11       Impact factor: 4.792

3.  Specificity of action on mosquito larvae of Bacillus thuringiensis israelensis toxins encoded by two different genes.

Authors:  A Delécluse; C Bourgouin; A Klier; G Rapoport
Journal:  Mol Gen Genet       Date:  1988-09

4.  Molecular characterization of a gene encoding a 72-kilodalton mosquito-toxic crystal protein from Bacillus thuringiensis subsp. israelensis.

Authors:  W P Donovan; C Dankocsik; M P Gilbert
Journal:  J Bacteriol       Date:  1988-10       Impact factor: 3.490

Review 5.  Bacillus thuringiensis and related insect pathogens.

Authors:  A I Aronson; W Beckman; P Dunn
Journal:  Microbiol Rev       Date:  1986-03

6.  Effect of the media composition on the growth parameters and biological properties ofBacillus thuringiensis var.israelensis delta-endotoxin.

Authors:  M M Faloci; O M Yantorno; H A Marino; J A Arcas; R J Ertola
Journal:  World J Microbiol Biotechnol       Date:  1990-03       Impact factor: 3.312

7.  Immunological relationships among proteins making up the Bacillus thuringiensis subsp. israelensis crystalline toxin.

Authors:  M A Pfannenstiel; G A Couche; E J Ross; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1986-10       Impact factor: 4.792

8.  Isolation of a relatively nontoxic 65-kilodalton protein inclusion from the parasporal body of Bacillus thuringiensis subsp. israelensis.

Authors:  J E Ibarra; B A Federici
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490

9.  Stability of the larvicidal activity of Bacillus thuringiensis subsp. israelensis: amino acid modification and denaturants.

Authors:  M A Pfannenstiel; G A Couche; G Muthukumar; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1985-11       Impact factor: 4.792

10.  Structural disulfide bonds in the Bacillus thuringiensis subsp. israelensis protein crystal.

Authors:  G A Couche; M A Pfannenstiel; K W Nickerson
Journal:  J Bacteriol       Date:  1987-07       Impact factor: 3.490

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