Chromosome Segregation in Fertilized Eggs From Triploid Pacific Oysters, Crassostrea gigas (Thunberg), Following Inhibition of Polar Body 1.

Chromosome segregation in fertilized eggs from triploid Pacific oysters, following inhibition of the first polar body (PB1), was studied with acetic orcein staining techniques. To block the release of PB1, fertilized eggs were treated with 0.5 mg/l of cytochalasin B (CB). Four types of segregation were observed, namely, "tripolar segregation" (54.5%), "united bipolar segregation" (12%), "separated bipolar segregation" (2.5%), and "incomplete united bipolar segregation" (4%). The remaining 23% could not be classified because of chromosome disorganization, but appeared to be variants of the above. It seemed clear that the predominant pattern that gave rise to tetraploids was united bipolar segregation, although certain separated bipolar segregations might also lead to the formation of tetraploids. The sequential events of meioses observed in CB-treated eggs are described. The asynchrony of meiotic events and possible mechanisms for the various types of chromosome segregation are discussed.