Masanobu Mizuta1,2, Takashi Kurita2, Neal P Dillon3, Emily E Kimball4, C Gaelyn Garrett2, M Preeti Sivasankar5, Robert J Webster2,6,7,3,8, Bernard Rousseau2,4,3,5. 1. Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan. 2. Department of Otolaryngology. 3. Department of Mechanical Engineering. 4. Department of Hearing and Speech Sciences. 5. Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana, U.S.A. 6. Department of Neurological Surgery. 7. Department of Urologic Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, U.S.A. 8. Department of Electrical Engineering, Vanderbilt University, Nashville, Tennessee, U.S.A.
Abstract
OBJECTIVES/HYPOTHESIS: A custom-designed probe was developed to measure vocal fold surface resistance in vivo. The purpose of this study was to demonstrate proof of concept of using vocal fold surface resistance as a proxy of functional tissue integrity after acute phonotrauma using an animal model. STUDY DESIGN: Prospective animal study. METHODS: New Zealand White breeder rabbits received 120 minutes of airflow without vocal fold approximation (control) or 120 minutes of raised intensity phonation (experimental). The probe was inserted via laryngoscope and placed on the left vocal fold under endoscopic visualization. Vocal fold surface resistance of the middle one-third of the vocal fold was measured after 0 (baseline), 60, and 120 minutes of phonation. After the phonation procedure, the larynx was harvested and prepared for transmission electron microscopy. RESULTS: In the control group, vocal fold surface resistance values remained stable across time points. In the experimental group, surface resistance (X% ± Y% relative to baseline) was significantly decreased after 120 minutes of raised intensity phonation. This was associated with structural changes using transmission electron microscopy, which revealed damage to the vocal fold epithelium after phonotrauma, including disruption of the epithelium and basement membrane, dilated paracellular spaces, and alterations to epithelial microprojections. In contrast, control vocal fold specimens showed well-preserved stratified squamous epithelia. CONCLUSIONS: These data demonstrate the feasibility of measuring vocal fold surface resistance in vivo as a means of evaluating functional vocal fold epithelial barrier integrity. Device prototypes are in development for additional testing, validation, and for clinical applications in laryngology. LEVEL OF EVIDENCE: NA Laryngoscope, 127:E364-E370, 2017.
OBJECTIVES/HYPOTHESIS: A custom-designed probe was developed to measure vocal fold surface resistance in vivo. The purpose of this study was to demonstrate proof of concept of using vocal fold surface resistance as a proxy of functional tissue integrity after acute phonotrauma using an animal model. STUDY DESIGN: Prospective animal study. METHODS: New Zealand White breeder rabbits received 120 minutes of airflow without vocal fold approximation (control) or 120 minutes of raised intensity phonation (experimental). The probe was inserted via laryngoscope and placed on the left vocal fold under endoscopic visualization. Vocal fold surface resistance of the middle one-third of the vocal fold was measured after 0 (baseline), 60, and 120 minutes of phonation. After the phonation procedure, the larynx was harvested and prepared for transmission electron microscopy. RESULTS: In the control group, vocal fold surface resistance values remained stable across time points. In the experimental group, surface resistance (X% ± Y% relative to baseline) was significantly decreased after 120 minutes of raised intensity phonation. This was associated with structural changes using transmission electron microscopy, which revealed damage to the vocal fold epithelium after phonotrauma, including disruption of the epithelium and basement membrane, dilated paracellular spaces, and alterations to epithelial microprojections. In contrast, control vocal fold specimens showed well-preserved stratified squamous epithelia. CONCLUSIONS: These data demonstrate the feasibility of measuring vocal fold surface resistance in vivo as a means of evaluating functional vocal fold epithelial barrier integrity. Device prototypes are in development for additional testing, validation, and for clinical applications in laryngology. LEVEL OF EVIDENCE: NA Laryngoscope, 127:E364-E370, 2017.
Authors: Elif Saritas Yuksel; Tina Higginbotham; James C Slaughter; Jerry Mabary; Robert T Kavitt; C Gaelyn Garrett; Michael F Vaezi Journal: Clin Gastroenterol Hepatol Date: 2012-05-27 Impact factor: 11.382
Authors: Bernard Rousseau; Tsuyoshi Kojima; Carolyn K Novaleski; Emily E Kimball; Carla V Valenzuela; Masanobu Mizuta; James J Daniero; C Gaelyn Garrett; M Preeti Sivasankar Journal: Cells Tissues Organs Date: 2017-06-24 Impact factor: 2.481
Authors: Tsuyoshi Kojima; Mark Van Deusen; W Gray Jerome; C Gaelyn Garrett; M Preeti Sivasankar; Carolyn K Novaleski; Bernard Rousseau Journal: PLoS One Date: 2014-03-13 Impact factor: 3.240