Literature DB >> 28573612

Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems.

Tessa Cressey1, Kristina Brauburger1,2, Elke Mühlberger3.   

Abstract

In this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.

Entities:  

Keywords:  Ebola virus; Filoviruses; Firefly luciferase assay; Minigenome; Replication; Replication promoter; Reporter gene; Transcription; Zaire ebolavirus; β-galactosidase enzyme assay

Mesh：

Substances：

Year:  2017        PMID： 28573612     DOI： 10.1007/978-1-4939-7116-9_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

Review 1.  Status of antiviral therapeutics against rabies virus and related emerging lyssaviruses.

Authors:  Venice Du Pont; Richard K Plemper; Matthias J Schnell
Journal:  Curr Opin Virol       Date:  2019-02-10       Impact factor: 7.090

2.  Comparison of Zaire and Bundibugyo Ebolavirus Polymerase Complexes and Susceptibility to Antivirals through a Newly Developed Bundibugyo Minigenome System.

Authors:  Corri B Levine; Chad E Mire; Thomas W Geisbert
Journal:  J Virol       Date:  2021-08-11       Impact factor: 5.103

3.  Engineered Human Cathelicidin Antimicrobial Peptides Inhibit Ebola Virus Infection.

Authors:  Yangsheng Yu; Christopher L Cooper; Guangshun Wang; M Jane Morwitzer; Krishna Kota; Julie P Tran; Steven B Bradfute; Yan Liu; Jiayu Shao; Amanda K Zhang; Lindsey G Luo; St Patrick Reid; Steven H Hinrichs; Kaihong Su
Journal:  iScience       Date:  2020-03-22

4.  Variation around the dominant viral genome sequence contributes to viral load and outcome in patients with Ebola virus disease.

Authors:  Xiaofeng Dong; Jordana Munoz-Basagoiti; Natasha Y Rickett; Georgios Pollakis; William A Paxton; Stephan Günther; Romy Kerber; Lisa F P Ng; Michael J Elmore; N'faly Magassouba; Miles W Carroll; David A Matthews; Julian A Hiscox
Journal:  Genome Biol       Date:  2020-09-07       Impact factor: 13.583
  4 in total

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