| Literature DB >> 28573577 |
Abstract
Time-resolved macromolecular crystallography unifies protein structure determination with chemical kinetics. With the advent of fourth generation X-ray sources the time-resolution can be on the order of 10-40 fs, which opens the ultrafast time scale to structure determination. Fundamental motions and transitions associated with chemical reactions in proteins can now be observed. Moreover, new experimental approaches at synchrotrons allow for the straightforward investigation of all kind of reactions in biological macromolecules. Here, recent developments in the field are reviewed.Keywords: Chemical kinetics; Structure based enzymology; Time-resolved macromolecular crystallography; Time-resolved serial femtosecond crystallography
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Year: 2017 PMID: 28573577 DOI: 10.1007/978-1-4939-7000-1_11
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745