Yamato Mizobe1,2, Yuji Ezono3, Makoto Tokunaga3, Naoto Oya3, Reiko Iwakiri3, Naomi Yoshida3, Yumi Sato3, Nanase Onoue3, Kazuchika Miyoshi4. 1. Aiiku Ladies Clinic, 1-40-2 Komatsubara, Kagoshima, 891-0114, Japan. lca.aiiku.lab.3f@gmail.com. 2. The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan. lca.aiiku.lab.3f@gmail.com. 3. Aiiku Ladies Clinic, 1-40-2 Komatsubara, Kagoshima, 891-0114, Japan. 4. The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan.
Abstract
PURPOSE: Recently, we established a noninvasive system for selecting human blastocysts with a high pre-transfer implantation potential based on first and second division patterns. The present study was carried out to improve the selection system. METHODS: Embryos that completed first and second divisions within 25.90 and 37.88 h after culture, respectively, were selected using a time-lapse incubator. We examined the effects of compaction and blastocyst formation times on pregnancy rates after transferring these embryos at the blastocyst stage. RESULTS: The completion of compaction and blastocyst formation times (79.93 and 97.47 h after culture, respectively) of embryos resulting in pregnancies after transfer were significantly (P < 0.01) shorter than those (86.46 and 100.34 h after culture, respectively) of embryos that failed to induce pregnancies. Embryo selection based on completion of compaction time improved pregnancy rates (40.9 vs. 74.6%, P < 0.01). CONCLUSIONS: Of the embryos that formed two cells during the first division within 25.90 h after culture and four cells during the second division within 37.88 h after culture, those that completed compaction within 79.93 h after culture before reaching the blastocyst stage had a high implantation potential.
PURPOSE: Recently, we established a noninvasive system for selecting human blastocysts with a high pre-transfer implantation potential based on first and second division patterns. The present study was carried out to improve the selection system. METHODS: Embryos that completed first and second divisions within 25.90 and 37.88 h after culture, respectively, were selected using a time-lapse incubator. We examined the effects of compaction and blastocyst formation times on pregnancy rates after transferring these embryos at the blastocyst stage. RESULTS: The completion of compaction and blastocyst formation times (79.93 and 97.47 h after culture, respectively) of embryos resulting in pregnancies after transfer were significantly (P < 0.01) shorter than those (86.46 and 100.34 h after culture, respectively) of embryos that failed to induce pregnancies. Embryo selection based on completion of compaction time improved pregnancy rates (40.9 vs. 74.6%, P < 0.01). CONCLUSIONS: Of the embryos that formed two cells during the first division within 25.90 h after culture and four cells during the second division within 37.88 h after culture, those that completed compaction within 79.93 h after culture before reaching the blastocyst stage had a high implantation potential.
Entities:
Keywords:
Compaction; Morula; Single blastocyst transfer; Time-lapse incubator
Authors: C Giorgetti; E Hans; P Terriou; J Salzmann; B Barry; V Chabert-Orsini; J M Chinchole; J P Franquebalme; E Glowaczower; M-C Sitri; M-C Thibault; R Roulier Journal: Reprod Biomed Online Date: 2007-01 Impact factor: 3.828
Authors: Susanna Apter; Thomas Ebner; Thomas Freour; Yves Guns; Borut Kovacic; Nathalie Le Clef; Monica Marques; Marcos Meseguer; Debbie Montjean; Ioannis Sfontouris; Roger Sturmey; Giovanni Coticchio Journal: Hum Reprod Open Date: 2020-03-19