Literature DB >> 28573167

Preparation of Purified Gram-positive Bacterial Cell Wall and Detection in Placenta and Fetal Tissues.

Beth Mann1, Lip Nam Loh1, Geli Gao1, Elaine Tuomanen1.   

Abstract

Cell wall is a complex biopolymer on the surface of all Gram-positive bacteria. During infection, cell wall is recognized by the innate immune receptor Toll-like receptor 2 causing intense inflammation and tissue damage. In animal models, cell wall traffics from the blood stream to many organs in the body, including brain, heart, placenta and fetus. This protocol describes how to prepare purified cell wall from Streptococcus pneumoniae, detect its distribution in animal tissues, and study the tissue response using the placenta and fetal brain as examples.

Entities:  

Keywords:  Bacterial inflammation; Cell wall; Fetal neurogenesis; Neuroproliferation; Peptidoglycan; Placental trafficking; Streptococcus pneumonae; Toll like receptor 2 ligand

Year:  2016        PMID： 28573167      PMCID： PMC5448413          DOI： 10.21769/BioProtoc.2037

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

1.  Cell wall-mediated neuronal damage in early sepsis.

Authors:  Carlos J Orihuela; Sophie Fillon; S Hope Smith-Sielicki; Karim C El Kasmi; Geli Gao; Konstantinos Soulis; Avinash Patil; Peter J Murray; Elaine I Tuomanen
Journal:  Infect Immun       Date:  2006-07       Impact factor: 3.441

2.  Bacterial Peptidoglycan Traverses the Placenta to Induce Fetal Neuroproliferation and Aberrant Postnatal Behavior.

Authors:  Jessica Humann; Beth Mann; Geli Gao; Philip Moresco; Joseph Ramahi; Lip Nam Loh; Arden Farr; Yunming Hu; Kelly Durick-Eder; Sophie A Fillon; Richard J Smeyne; Elaine I Tuomanen
Journal:  Cell Host Microbe       Date:  2016-06-08       Impact factor: 21.023

3.  Platelet-activating factor receptor and innate immunity: uptake of gram-positive bacterial cell wall into host cells and cell-specific pathophysiology.

Authors:  Sophie Fillon; Konstantinos Soulis; Surender Rajasekaran; Heather Benedict-Hamilton; Jana N Radin; Carlos J Orihuela; Karim C El Kasmi; Gopal Murti; Deepak Kaushal; M Waleed Gaber; Joerg R Weber; Peter J Murray; Elaine I Tuomanen
Journal:  J Immunol       Date:  2006-11-01       Impact factor: 5.422

4.  Specific recognition of choline residues in the cell wall teichoic acid by the N-acetylmuramyl-L-alanine amidase of Pneumococcus.

Authors:  J V Höltje; A Tomasz
Journal:  J Biol Chem       Date:  1975-08-10       Impact factor: 5.157

5.  Cutting edge: recognition of Gram-positive bacterial cell wall components by the innate immune system occurs via Toll-like receptor 2.

Authors:  A Yoshimura; E Lien; R R Ingalls; E Tuomanen; R Dziarski; D Golenbock
Journal:  J Immunol       Date:  1999-07-01       Impact factor: 5.422

6.  Neuroprotection by a caspase inhibitor in acute bacterial meningitis.

Authors:  J S Braun; R Novak; K H Herzog; S M Bodner; J L Cleveland; E I Tuomanen
Journal:  Nat Med       Date:  1999-03       Impact factor: 53.440

7.  The relative role of bacterial cell wall and capsule in the induction of inflammation in pneumococcal meningitis.

Authors:  E Tuomanen; A Tomasz; B Hengstler; O Zak
Journal:  J Infect Dis       Date:  1985-03       Impact factor: 5.226

8.  The induction of meningeal inflammation by components of the pneumococcal cell wall.

Authors:  E Tuomanen; H Liu; B Hengstler; O Zak; A Tomasz
Journal:  J Infect Dis       Date:  1985-05       Impact factor: 5.226
  8 in total
  2 in total

1.  Analysis of Gram-negative Bacteria Peptidoglycan by Ultra-performance Liquid Chromatography.

Authors:  Laura Alvarez; Baptiste Cordier; Sven Van Teeffelen; Felipe Cava
Journal:  Bio Protoc       Date:  2020-10-05

2.  Bacterial peptidoglycan muropeptides benefit mitochondrial homeostasis and animal physiology by acting as ATP synthase agonists.

Authors:  Dong Tian; Min Han
Journal:  Dev Cell       Date:  2022-01-18       Impact factor: 12.270
  2 in total

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