The significance of multiple CNS opioid receptor types: a review of critical considerations relating to technical details and anatomy in the study of central opioid actions.

The study of the CNS actions of opioids is complicated by the presence of both multiple opioid receptors and endogenous ligands in the brain. The recent descriptions of opioid isoreceptors, of tonic opioid systems, and of multiple opioid receptors on a single neuron are further technical details which must be considered. In the use of various opiates and opioid peptides to study physiological systems, the multiple opioid affinities of these compounds, as well as potential non-opioid actions, must be controlled for in the experimental design. In conjunction with the multiple receptor affinities of various opiates is the problem of receptor dualism with some drugs; particularly with the agonist/antagonist analgesics. Species differences in the relative proportions of different opioid receptor populations also limit any generalizations of a finding in one species. These limitations in the study of opioid receptors will be discussed with reference to previous neurochemical, neuroendocrine, electrophysiological and behavioral reports of multiple opioid receptors.