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Literature DB >> 2856437

An E. coli-yeast shuttle cosmid with positive selection for inserted fragments.

M E Gent¹, P Crowley, J R Ludwig, R Anwar, D A Sugden, P F Sims, S G Oliver.

Abstract

We describe the construction of a cosmid cloning vector, pMT555, which allows positive selection for the presence of an inserted DNA fragment. The vector contains sequences which enable its replication and selection in either E. coli or Saccharomyces cerevisiae. We demonstrate that pMT555 may be used for the efficient construction of total genomic banks from small quantities of donor DNA. The positive selection permits the stable maintenance of the cosmid in E. coli and the faithful replication of inserted sequences.

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Year: 1985 PMID： 2856437 DOI： 10.1007/bf00418490

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

13 in total

1. Isolation and characterisation of genes for sulphate activation and reduction in Aspergillus nidulans: implications for evolution of an allosteric control region by gene duplication.

Authors: M I Borges-Walmsley; G Turner; A M Bailey; J Brown; J Lehmbeck; I G Clausen
Journal: Mol Gen Genet Date: 1995-05-20

1 in total

An E. coli-yeast shuttle cosmid with positive selection for inserted fragments.

1. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA.

2. High-frequency transformation of the fission yeast Schizosaccharomyces pombe.

3. The effect of amino acids on growth and phosphate metabolism in a prototrophic yeast strain.

4. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

5. Rapid and efficient cosmid cloning.

6. A small cosmid for efficient cloning of large DNA fragments.

7. A plasmid cloning vehicle allowing a positive selection for inserted fragments.

8. Absence of short-period repetitive-sequence interspersion in the basidiomycete Schizophyllum commune.

9. Functional genetic expression of eukaryotic DNA in Escherichia coli.

10. Plasmid vectors capable of transferring large DNA fragments to yeast.

1. Isolation and characterisation of genes for sulphate activation and reduction in Aspergillus nidulans: implications for evolution of an allosteric control region by gene duplication.