Douadi Benacer1, Siti Nursheena Mohd Zain1, John W Lewis2, Mohd Khairul Nizam Mohd Khalid3, Kwai Lin Thong1. 1. Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia. 2. School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, United Kingdom. 3. Bacteriology Unit, Institute for Medical Research, Ministry of Health, Kuala Lumpur, Malaysia.
Abstract
INTRODUCTION: : This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: : Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. RESULTS: : The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water. CONCLUSIONS: : Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.
INTRODUCTION: : This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: : Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. RESULTS: : The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water. CONCLUSIONS: : Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.
Authors: Ada Agustina Sandoval-Carrillo; José Manuel Salas-Pacheco; Elizabeth Irasema Antuna-Salcido; Karla Sujey Castro-Martínez; Diana Stephanie Ortiz-Montaño; Isabel Beristain-Garcia; Hilda Mariela Alvarado-Retana; Agar Ramos-Nevarez; Sergio Manuel Salas-Pacheco; Antonio Sifuentes-Alvarez; Elizabeth Rábago-Sánchez; Sandra Margarita Cerrillo-Soto; Francisco Xavier Castellanos-Juárez; Edith Contreras-Cisneros; Cosme Alvarado-Esquivel Journal: J Int Med Res Date: 2021-04 Impact factor: 1.671
Authors: Shabani Kiyabo Motto; Gabriel Mkilema Shirima; Barend Mark de Clare Bronsvoort; Elizabeth Anne Jessie Cook Journal: PLoS Negl Trop Dis Date: 2021-11-16