A benign alternative process for efficient separation of pure commercially important flavonoid nutraceuticals from edible plants.

The present study signifies the development of a unique, optimized procedure for both selective determination and separation of different flavonoid nutraceuticals from edible plants. Totally ten different flavonoids were determined (HPLC-DAD) and isolated from five different plants using the developed process with a remarkable purity of 91-98% and recovery of 88-95%. Box-Behnken experimental design model yielded a optimized amount of 40.36 mg/g of AI extract (Pinostrobin) and 28.95 mg/g of AI extract (Baicalein) with a high correlation coefficient (0.98-0.99), indicating a good fit between the second order regression model and the experimental observations. The final purity of compounds through optimized process is 97.1% (Pinostrobin) and 93.5% (Baicalein) respectively. The optimized yields depicted a total recovery of 92% for pinostrobin, and 89% for Baicalein respectively. Thus, the developed process worked as a potential alternative which when statistically optimized results in a remarkable recovery of flavonoids from various plants. Being an environmentally friendly protocol the process could be useful in industrial separation of commercially important flavonoids widely applied in food industry.