Jie Li1, Yohitsugu Mitani1, Pulivarthi H Rao2, Laszlo Perlaky2, Bin Liu3, Randal S Weber4, Adel K El-Naggar5. 1. The University of Texas, M.D. Anderson Cancer Center, Department of Pathology, United States. 2. Baylor College of Medicine, Department of Pediatrics, Texas Children's Cancer and Hematology Centers, United States. 3. The University of Texas, M.D. Anderson Cancer Center, Department of Genetics, United States. 4. The University of Texas, M.D. Anderson Cancer Center, Department of Head and Neck Surgery, United States. 5. The University of Texas, M.D. Anderson Cancer Center, Department of Pathology, United States. Electronic address: anaggar@mdanderson.org.
Abstract
OBJECTIVES: To develop and characterize in vitro salivary duct carcinoma as a surrogate for functional studies. MATERIALS AND METHODS: Cells were dispersed from tumor tissue fragments under sterile conditions in RPMI media. Disassociated cells were cultivated, immortalized with hTERT and propagated for more than 100 passages. Morphologic, linage, cytogenetic and genomic analyses were performed on different passages of cell line and primary tumor. Soft agar growth was performed. RESULTS: Analysis of cytomorphologic features, growth characteristics and lineage specific markers expression confirmed the epithelial derivation and the neoplastic nature of the cell line. DNA STRs analysis showed identical match of both cell line and primary tumor. Cultivated cells expressed Androgen Receptor (AR), PTEN, and EFGR proteins and the AR-V7 isoform transcript. Comparative exome-sequencing identified common mutated genes in both cell line and primary tumor. In-vitro colony formation of late passages is established. CONCLUSION: We report the development of the first human salivary duct carcinoma cell line (MDA-SDC-04) that retains critical biological and genomic features of the donor tumor.
OBJECTIVES: To develop and characterize in vitro salivary duct carcinoma as a surrogate for functional studies. MATERIALS AND METHODS: Cells were dispersed from tumor tissue fragments under sterile conditions in RPMI media. Disassociated cells were cultivated, immortalized with hTERT and propagated for more than 100 passages. Morphologic, linage, cytogenetic and genomic analyses were performed on different passages of cell line and primary tumor. Soft agar growth was performed. RESULTS: Analysis of cytomorphologic features, growth characteristics and lineage specific markers expression confirmed the epithelial derivation and the neoplastic nature of the cell line. DNA STRs analysis showed identical match of both cell line and primary tumor. Cultivated cells expressed Androgen Receptor (AR), PTEN, and EFGR proteins and the AR-V7 isoform transcript. Comparative exome-sequencing identified common mutated genes in both cell line and primary tumor. In-vitro colony formation of late passages is established. CONCLUSION: We report the development of the first humansalivary duct carcinoma cell line (MDA-SDC-04) that retains critical biological and genomic features of the donortumor.
Authors: Yoshitsugu Mitani; Pulivarthi H Rao; Sankar N Maity; Yu-Chen Lee; Renata Ferrarotto; Julian C Post; Lisa Licitra; Scott M Lippman; Merrill S Kies; Randal S Weber; Carlos Caulin; Sue-Hwa Lin; Adel K El-Naggar Journal: Clin Cancer Res Date: 2014-10-14 Impact factor: 12.531
Authors: Li Zhang; Yoshitsugu Mitani; Carlos Caulin; Pulivarthi H Rao; Merrill S Kies; Pierre Saintigny; Nianxiang Zhang; Randal S Weber; Scott M Lippman; Adel K El-Naggar Journal: Am J Pathol Date: 2013-04-10 Impact factor: 4.307
Authors: J Chad Brenner; Martin P Graham; Bhavna Kumar; Lindsay M Saunders; Robbi Kupfer; Robert H Lyons; Carol R Bradford; Thomas E Carey Journal: Head Neck Date: 2010-04 Impact factor: 3.147
Authors: Samuel L Volchenboum; Cheng Li; Shuli Li; Edward F Attiyeh; C Patrick Reynolds; John M Maris; A Thomas Look; Rani E George Journal: Cancer Res Date: 2009-05-12 Impact factor: 12.701
Authors: Yoshitsugu Mitani; Sue-Hwa Lin; Kristen B Pytynia; Renata Ferrarotto; Adel K El-Naggar Journal: Clin Cancer Res Date: 2019-11-26 Impact factor: 12.531