Han Liu1, Bo Sun2, Shengnan Wang1, Congjin Liu1, Yun Lu1, Ding Li1, Xingdang Liu1. 1. Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai, China. 2. Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Abstract
BACKGROUND/AIMS: Circulating tumor cells (CTCs) are valuable in both basic research and clinical application for cancer management. In the current study, we evaluated the diagnostic value of CTCs in pancreatic ductal adenocarcinoma (PDAC). METHODS: In total, 143 blood samples from 95 consecutively diagnosed PDAC patients and 48 healthy donors were collected. Combined data from immunostaining of CD45, DAPI and fluorescence in situ hybridization (FISH) with chromosome 8 centromere (CEP8) probe were used to identify CTCs. Cells with features of CD45-/DAPI+/CEP8>2 were detected as CTCs. RESULTS: CTCs were classified as triploid, tetraploid and multiploid based on chromosome 8 copy number. CTC subtype composition was significantly different among groups. Both subtype number and total CTC number were significantly increased in PDAC patients, compared to healthy controls. Total CTC number had 75.8% sensitivity and 68.7% specificity at a cutoff value of 2 cells/3.2 mL. This study is the first to report that CTC subtype number is also useful in cancer diagnosis. Sensitivity was 53.7% and specificity was 85.4% at a cutoff point of 2 CTC subtypes. The diagnostic value of both total CTC number and CTC subtype number was a little poorer than CA199. CONCLUSIONS: Both CTC subtype and total CTC number may serve as potential biomarkers for PDAC.
BACKGROUND/AIMS: Circulating tumor cells (CTCs) are valuable in both basic research and clinical application for cancer management. In the current study, we evaluated the diagnostic value of CTCs in pancreatic ductal adenocarcinoma (PDAC). METHODS: In total, 143 blood samples from 95 consecutively diagnosed PDACpatients and 48 healthy donors were collected. Combined data from immunostaining of CD45, DAPI and fluorescence in situ hybridization (FISH) with chromosome 8 centromere (CEP8) probe were used to identify CTCs. Cells with features of CD45-/DAPI+/CEP8>2 were detected as CTCs. RESULTS: CTCs were classified as triploid, tetraploid and multiploid based on chromosome 8 copy number. CTC subtype composition was significantly different among groups. Both subtype number and total CTC number were significantly increased in PDACpatients, compared to healthy controls. Total CTC number had 75.8% sensitivity and 68.7% specificity at a cutoff value of 2 cells/3.2 mL. This study is the first to report that CTC subtype number is also useful in cancer diagnosis. Sensitivity was 53.7% and specificity was 85.4% at a cutoff point of 2 CTC subtypes. The diagnostic value of both total CTC number and CTC subtype number was a little poorer than CA199. CONCLUSIONS: Both CTC subtype and total CTC number may serve as potential biomarkers for PDAC.
Authors: Seung Joon Lee; Tae Seok Sim; Hyun Young Shin; Jungmin Lee; Min Young Kim; Joseph Sunoo; Jeong-Gun Lee; Kyungmoo Yea; Young Zoon Kim; Danny van Noort; Soo Kyung Park; Woon-Hae Kim; Kyun Woo Park; Minseok S Kim Journal: PLoS One Date: 2019-10-24 Impact factor: 3.240
Authors: Rossana Signorelli; Teresa Maidana Giret; Oliver Umland; Marco Hadisurya; Shweta Lavania; John Lalith Charles Richard; Ashley Middleton; Melinda Minucci Boone; Ayse Burcu Ergonul; Weiguo Andy Tao; Haleh Amirian; Anton Iliuk; Aliya Khan; Robert Diaz; Daniel Bilbao Cortes; Monica Garcia-Buitrago; Harrys Kishore Charles Jacob Journal: Biomedicines Date: 2022-08-16