María Roca1, Sabine Lisa Vriezinga2, Paula Crespo-Escobar3, Renata Auricchio4, David Hervás5, Gemma Castillejo6, Maria Carmen Mena7, Isabel Polanco8, Riccardo Troncone4, Maria Luisa Mearin2, Carmen Ribes-Koninckx3,9. 1. Medical Research Institute La Fe, Unidad de Enfermedad Celíaca e Inmunopatología Digestiva, Torre A, 6º Planta. Avda. Fernando Abril Martorell 106, 46026, Valencia, Spain. maria_roca@iislafe.es. 2. Department of Paediatrics, Leiden University Medical Centre, Leiden, The Netherlands. 3. Medical Research Institute La Fe, Unidad de Enfermedad Celíaca e Inmunopatología Digestiva, Torre A, 6º Planta. Avda. Fernando Abril Martorell 106, 46026, Valencia, Spain. 4. Department of Medical Translational Sciences and European Laboratory for the Investigation of Food-Induced Diseases, University Federico II, Naples, Italy. 5. Biostatistics Unit, Medical Research Institute La Fe, Valencia, Spain. 6. Department of Pediatric Gastroenterology Unit, Hospital Universitari Sant Joan de Reus, URV, IIPV, Reus, Spain. 7. Proteomics Facility, Centro Nacional Biotecnología, CSIC, Madrid, Spain. 8. Department of Pediatric Gastroenterology and Nutrition, La Paz University Hospital, Madrid, Spain. 9. Pediatric Gastroenterology, Hospital Universitari i Politècnic La Fe, Valencia, Spain.
Abstract
PURPOSE: To analyze the presence of total IgA and anti-gliadin antibodies (AGA) in BM from CD mothers who follow a gluten-free diet (GFD) and from mothers on a normal gluten-containing diet (ND). METHODS: 218 samples of mature milk were obtained at different months of lactation (1-6) from 83 mothers (2 or more samples per mother) from Italy (Naples), The Netherlands (Leiden) and Spain (Madrid, Valencia and Reus): 42 CD mothers on GFD for more than 2 years and 41 non-CD mothers on a ND. Whey samples were analyzed for AGA-IgA by an indirect homemade ELISA and for total IgA (g/L) by a commercial ELISA kit. RESULTS: AGA-IgA was detected in BM, both in mothers on a GFD and mothers on a ND. AGA-IgA levels in both groups of mothers, CD and non-CD, show the same trend towards decreasing slightly along the months of lactation (p = 0.91). A different trend is observed for total IgA levels, decreasing markedly in CD mothers from the first month of lactation onwards but remaining stable in non-CD mothers (p = 0.048). A statistically significant association was found between the means of total IgA and AGA-IgA (p < 0.001). CONCLUSION: AGA-IgA is present in BM from mothers on a ND as well as in BM from mothers who had been on a GFD for years. This reflects the existence of a long-lasting immunological memory independent of the mother's diet. If the presence of these antibodies has any role in promoting the acquisition of gluten tolerance in the infant, our study shows that children of CD mothers would be on equal conditions as children of non-CD mothers.
RCT Entities:
PURPOSE: To analyze the presence of total IgA and anti-gliadin antibodies (AGA) in BM from CD mothers who follow a gluten-free diet (GFD) and from mothers on a normal gluten-containing diet (ND). METHODS: 218 samples of mature milk were obtained at different months of lactation (1-6) from 83 mothers (2 or more samples per mother) from Italy (Naples), The Netherlands (Leiden) and Spain (Madrid, Valencia and Reus): 42 CD mothers on GFD for more than 2 years and 41 non-CD mothers on a ND. Whey samples were analyzed for AGA-IgA by an indirect homemade ELISA and for total IgA (g/L) by a commercial ELISA kit. RESULTS: AGA-IgA was detected in BM, both in mothers on a GFD and mothers on a ND. AGA-IgA levels in both groups of mothers, CD and non-CD, show the same trend towards decreasing slightly along the months of lactation (p = 0.91). A different trend is observed for total IgA levels, decreasing markedly in CD mothers from the first month of lactation onwards but remaining stable in non-CD mothers (p = 0.048). A statistically significant association was found between the means of total IgA and AGA-IgA (p < 0.001). CONCLUSION: AGA-IgA is present in BM from mothers on a ND as well as in BM from mothers who had been on a GFD for years. This reflects the existence of a long-lasting immunological memory independent of the mother's diet. If the presence of these antibodies has any role in promoting the acquisition of gluten tolerance in the infant, our study shows that children of CD mothers would be on equal conditions as children of non-CD mothers.
Entities:
Keywords:
Breast milk; Celiac disease; Gliadin antibodies; Immunoglobulin A
Authors: F Mascart-Lemone; P Donnen; B Paluku; D Brasseur; J Van den Broeck; J P Vaerman; P Hennart; J Duchateau Journal: Adv Exp Med Biol Date: 1991 Impact factor: 2.622
Authors: H Szajewska; R Shamir; A Chmielewska; M Pieścik-Lech; R Auricchio; A Ivarsson; S Kolacek; S Koletzko; I Korponay-Szabo; M L Mearin; C Ribes-Koninckx; R Troncone Journal: Aliment Pharmacol Ther Date: 2015-03-26 Impact factor: 8.171
Authors: S Auricchio; D Follo; G de Ritis; A Giunta; D Marzorati; L Prampolini; N Ansaldi; P Levi; D Dall'Olio; A Bossi Journal: J Pediatr Gastroenterol Nutr Date: 1983 Impact factor: 2.839