Vincent Thomas De Montpréville1, Maria-Rosa Ghigna2, Ludovic Lacroix3, Antoinette Lemoine4, Benjamin Besse5, Olaf Mercier6, Élie Fadel6, Peter Dorfmuller2, Thierry Le Chevalier7. 1. Department of Pathology, Marie Lannelongue Hospital, 133 Avenue de la Résistance, 92350, Le Plessis Robinson, France. Electronic address: v.thomasdemontpreville@ccml.fr. 2. Department of Pathology, Marie Lannelongue Hospital, 133 Avenue de la Résistance, 92350, Le Plessis Robinson, France. 3. Department of Pathology and Medical Biology, Gustave Roussy University Hospital, 114 rue Edouard Vaillant, 94805, Villejuif, France. 4. Department of Biochemistry and Oncogenetics, AP-HP, Paul Brousse Hospital, 12 Avenue Paul-Vaillant-Couturier, 94800, Villejuif, France. 5. Department of Medical Oncology, Gustave Roussy University Hospital, 114 rue Edouard Vaillant, 94805, Villejuif, France. 6. Department of Thoracic and Vascular Surgery and Heart-Lung Transplantation, Marie Lannelongue Hospital, 133 Avenue de la Résistance, 92350, Le Plessis Robinson, France. 7. Department of Medical Oncology, Gustave Roussy University Hospital, 114 rue Edouard Vaillant, 94805, Villejuif, France; Department of Thoracic and Vascular Surgery and Heart-Lung Transplantation, Marie Lannelongue Hospital, 133 Avenue de la Résistance, 92350, Le Plessis Robinson, France.
Abstract
OBJECTIVES: EGFR and KRAS genes are routinely tested in lung carcinomas with therapeutic implications. However the current testing methods require complex infrastructures and the delay for diagnosis remains often rather long, especially for initiating an appropriate treatment in patients with advanced stage tumor and short life expectancy. MATERIAL AND METHODS: We evaluated the Idylla™ fully automated molecular diagnostic system in routine conditions in 79 lung adenocarcinomas and 14 other non-small cell lung carcinomas, mostly in advanced stages (III or IV: 85%). Tests were performed on formalin-fixed paraffin-embedded (n=83) or fresh (n=10) material, including cytological (n=24) and small biopsy (n=20) samples. In prospective cases (n=82), the most likely mutated gene (EGFR in non or occasional smokers and KRAS in smokers) was tested first; the second gene being only tested in case of negativity. RESULTS: The system did not require complex training. Mutational status was obtained in few hours after making the histological diagnosis and on the day of the patient's sampling by analyzing fresh material. The sequential testing strategy avoided 15 EGFR and 15 KRAS tests that would have been negative. Compared with reference methods, global specificity and sensitivity were both 100% for EGFR mutations, and 89.1% and 91.7% for KRAS mutations, respectively. CONCLUSIONS: We demonstrated that such easy-to-use systems can permit pathologists to integrate a reliable EGFR/KRAS status in their initial pathologic report, and could be useful complementary tools to the current molecular diagnostic methods, with regard to prompt therapeutic management of lung cancer patients.
OBJECTIVES:EGFR and KRAS genes are routinely tested in lung carcinomas with therapeutic implications. However the current testing methods require complex infrastructures and the delay for diagnosis remains often rather long, especially for initiating an appropriate treatment in patients with advanced stage tumor and short life expectancy. MATERIAL AND METHODS: We evaluated the Idylla™ fully automated molecular diagnostic system in routine conditions in 79 lung adenocarcinomas and 14 other non-small cell lung carcinomas, mostly in advanced stages (III or IV: 85%). Tests were performed on formalin-fixed paraffin-embedded (n=83) or fresh (n=10) material, including cytological (n=24) and small biopsy (n=20) samples. In prospective cases (n=82), the most likely mutated gene (EGFR in non or occasional smokers and KRAS in smokers) was tested first; the second gene being only tested in case of negativity. RESULTS: The system did not require complex training. Mutational status was obtained in few hours after making the histological diagnosis and on the day of the patient's sampling by analyzing fresh material. The sequential testing strategy avoided 15 EGFR and 15 KRAS tests that would have been negative. Compared with reference methods, global specificity and sensitivity were both 100% for EGFR mutations, and 89.1% and 91.7% for KRAS mutations, respectively. CONCLUSIONS: We demonstrated that such easy-to-use systems can permit pathologists to integrate a reliable EGFR/KRAS status in their initial pathologic report, and could be useful complementary tools to the current molecular diagnostic methods, with regard to prompt therapeutic management of lung cancerpatients.
Authors: Maria-Rosa Ghigna; Adrian Crutu; Valentina Florea; Séverine Feuillet-Soummer; Pierre Baldeyrou; Julien Adam; Ludovic Lacroix; Benjamin Besse; Olaf Mercier; Elie Fadel; Peter Dorfmuller; Rida El Ayoubi; Vincent Thomas de Montpréville Journal: J Thorac Dis Date: 2018-07 Impact factor: 2.895
Authors: Henri B Wolff; Elisabeth M P Steeghs; Zakile A Mfumbilwa; Harry J M Groen; Eddy M Adang; Stefan M Willems; Katrien Grünberg; Ed Schuuring; Marjolijn J L Ligtenberg; Bastiaan B J Tops; Veerle M H Coupé Journal: JCO Precis Oncol Date: 2022-07
Authors: M Rabie Al-Turkmani; Michael A Suriawinata; Sophie J Deharvengt; Donald C Green; Candice C Black; Keisuke Shirai; Konstantin H Dragnev; Gregory J Tsongalis Journal: Pract Lab Med Date: 2020-03-02