Gerardo Cortés-Cortés1, Patricia Lozano-Zarain1, Carmen Torres2, Carla Andrea Alonso2, Ana María Ríos-Torres1, Miguel Castañeda1, Liliana López-Pliego1, Armando Navarro3, Rosa Del Carmen Rocha-Gracia4. 1. Benemérita Universidad Autónoma de Puebla, Posgrado en Microbiología, Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Ciudad Universitaria, San Manuel, 72570 Puebla, Mexico. 2. Universidad de La Rioja, Área Bioquímica y Biología Molecular, Madre de Dios 51, Logroño 26006, Spain. 3. Universidad Nacional Autónoma de México, Departamento de Salud Pública, Facultad de Medicina, Avenida Universidad 3000, Copilco Universidad, Cd. Universitaria, 04510 Ciudad de México, Mexico. 4. Benemérita Universidad Autónoma de Puebla, Posgrado en Microbiología, Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Ciudad Universitaria, San Manuel, 72570 Puebla, Mexico. Electronic address: rochagra@yahoo.com.
Abstract
OBJECTIVES: The resistance mechanisms, molecular type and plasmid content of cefotaxime-resistant Escherichia coli isolated from faecal samples of healthy volunteers in Puebla, Mexico, were characterised. METHODS AND RESULTS: Cefotaxime-resistant E. coli were recovered from 11 (18%) of 60 healthy volunteers. The isolates (one per sample) were characterised as multidrug-resistant and phenotypically extended-spectrum β-lactamase (ESBL)-producing strains. Genes encoding resistance to β-lactams (blaCTX-M-15, blaCTX-M-14a, blaCTX-M-14b, blaOXA-1, blaTEM-1b), quinolones [aac(6')-Ib-cr, qnrB19], aminoglycosides [aac(3')-II] and tetracycline [tet(A), tet(B)] were detected among the 11 ESBL-producing E. coli by PCR and sequencing, as well as gene cassette arrays in class 1 (dfrA17-aadA5) and class 2 (dfrA1-sat2-aadA1) integrons. Seven pulsotypes were identified by XbaI PFGE and the strains were distributed into phylogroups (number of isolates) A (2), B2 (4) and D (5). Seven sequence types were identified, four of them novel (ST5060, ST5079, ST5080 and ST5081), associated with phylogroups A-D. Transfer of a 140-kb IncFIA plasmid carrying the blaCTX-M-15 gene was evidenced in the ST5060 strain. Four CTX-M-15-producing E. coli strains of phylogroup B2 belonged to the ST131 complex, and IncFIB plasmids of 130kb and 155kb were detected in two of them. Multiple plasmid addiction systems were also found. Serotyping and fimH subtyping of ST131-B2 strains identified the ST131-B2-O25:H4-H30-Rx subclone. Additionally, this subclone and CTX-M-14-producing isolates were detected among residents living in the same household, suggesting clonal dissemination. CONCLUSIONS: This study reports the detection of E. coli ST131-B2-O25:H4-H30-Rx subclone in healthy humans in Mexico, highlighting its dissemination in the community setting.
OBJECTIVES: The resistance mechanisms, molecular type and plasmid content of cefotaxime-resistant Escherichia coli isolated from faecal samples of healthy volunteers in Puebla, Mexico, were characterised. METHODS AND RESULTS:Cefotaxime-resistant E. coli were recovered from 11 (18%) of 60 healthy volunteers. The isolates (one per sample) were characterised as multidrug-resistant and phenotypically extended-spectrum β-lactamase (ESBL)-producing strains. Genes encoding resistance to β-lactams (blaCTX-M-15, blaCTX-M-14a, blaCTX-M-14b, blaOXA-1, blaTEM-1b), quinolones [aac(6')-Ib-cr, qnrB19], aminoglycosides [aac(3')-II] and tetracycline [tet(A), tet(B)] were detected among the 11 ESBL-producing E. coli by PCR and sequencing, as well as gene cassette arrays in class 1 (dfrA17-aadA5) and class 2 (dfrA1-sat2-aadA1) integrons. Seven pulsotypes were identified by XbaI PFGE and the strains were distributed into phylogroups (number of isolates) A (2), B2 (4) and D (5). Seven sequence types were identified, four of them novel (ST5060, ST5079, ST5080 and ST5081), associated with phylogroups A-D. Transfer of a 140-kb IncFIA plasmid carrying the blaCTX-M-15 gene was evidenced in the ST5060 strain. Four CTX-M-15-producing E. coli strains of phylogroup B2 belonged to the ST131 complex, and IncFIB plasmids of 130kb and 155kb were detected in two of them. Multiple plasmid addiction systems were also found. Serotyping and fimH subtyping of ST131-B2 strains identified the ST131-B2-O25:H4-H30-Rx subclone. Additionally, this subclone and CTX-M-14-producing isolates were detected among residents living in the same household, suggesting clonal dissemination. CONCLUSIONS: This study reports the detection of E. coli ST131-B2-O25:H4-H30-Rx subclone in healthy humans in Mexico, highlighting its dissemination in the community setting.
Authors: Jay Vornhagen; Christine M Bassis; Srividya Ramakrishnan; Robert Hein; Sophia Mason; Yehudit Bergman; Nicole Sunshine; Yunfan Fan; Caitlyn L Holmes; Winston Timp; Michael C Schatz; Vincent B Young; Patricia J Simner; Michael A Bachman Journal: PLoS Pathog Date: 2021-04-30 Impact factor: 6.823
Authors: Edwin Barrios-Villa; Gerardo Cortés-Cortés; Patricia Lozano-Zaraín; Margarita María de la Paz Arenas-Hernández; Claudia Fabiola Martínez de la Peña; Ygnacio Martínez-Laguna; Carmen Torres; Rosa Del Carmen Rocha-Gracia Journal: Ann Clin Microbiol Antimicrob Date: 2018-12-10 Impact factor: 3.944
Authors: Evelyn Méndez-Moreno; Liliana Caporal-Hernandez; Pablo A Mendez-Pfeiffer; Yessica Enciso-Martinez; Rafael De la Rosa López; Dora Valencia; Margarita M P Arenas-Hernández; Manuel G Ballesteros-Monrreal; Edwin Barrios-Villa Journal: Antibiotics (Basel) Date: 2022-06-21