Harald Seeger1,2, Joerg Latus3, Daniel Kitterer3, M Dominik Alscher3, Dagmar Biegger4, Jin Chen1,2, Ilka Edenhofer1,2, Rudolf P Wüthrich1, Stephan Segerer5,6. 1. Division of Nephrology, University Hospital, Rämistr. 100, 8091, Zurich, Switzerland. 2. Institute of Physiology and Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland. 3. Division of General Internal Medicine and Nephrology, Department of Internal Medicine, Robert-Bosch-Hospital, Stuttgart, Germany. 4. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Stuttgart, Germany. 5. Division of Nephrology, University Hospital, Rämistr. 100, 8091, Zurich, Switzerland. Stephan.segerer@ksa.ch. 6. Division of Nephrology, Dialysis and Transplantation, Kantonsspital, Tellstrasse 25, 5001, Aarau, Switzerland. Stephan.segerer@ksa.ch.
Abstract
BACKGROUND: Peritoneal injury is an important cause of technical failure of long-term peritoneal dialysis (PD). Encapsulating peritoneal sclerosis (EPS) is a severe complication of long-term PD with potentially life threatening consequences. CD147 is a glycoprotein with diverse functions including modulation of extracellular matrix via induction of matrix metalloproteinases, cell adhesion, and regulation of immune reactions. We hypothesized that CD 147 plays a role in the peritoneal cavity. METHODS: In this retrospective study, we localized CD147 by immunohistochemistry in peritoneal biopsies from uremic patients not on PD (n = 8), on PD without signs of EPS (n = 7), and in biopsies in patients with the diagnosis of EPS (n = 7). Double immunofluorescence was used to co-localize α-smooth-muscle actin (α-SMA) and CD147 in selected biopsies from each group. Expression was scored semi-quantitatively. RESULTS: In biopsies from uremic controls, CD147 was prominently expressed in mesothelial cells, focally between fat cells and by some perivascular cells. In patients on PD, a similar distribution was present (although mesothelium was rarely conserved), with some focal accentuation. In EPS, layers of fibroblastic cells were positive for CD147. EPS biopsies demonstrated a significantly higher score in a blinded evaluation, compared to uremic patients. Cells expressing CD147 were α-SMA positive myofibroblasts as demonstrated by double immunofluorescence. Mean CD147 scores did not differ between patients with different transporter status. CONCLUSIONS: This is the first study demonstrating CD147 on a major part of fibroblastic cells in EPS. Future studies need to address the role of these cells in this severe complication of long-term PD.
BACKGROUND:Peritoneal injury is an important cause of technical failure of long-term peritoneal dialysis (PD). Encapsulating peritoneal sclerosis (EPS) is a severe complication of long-term PD with potentially life threatening consequences. CD147 is a glycoprotein with diverse functions including modulation of extracellular matrix via induction of matrix metalloproteinases, cell adhesion, and regulation of immune reactions. We hypothesized that CD 147 plays a role in the peritoneal cavity. METHODS: In this retrospective study, we localized CD147 by immunohistochemistry in peritoneal biopsies from uremic patients not on PD (n = 8), on PD without signs of EPS (n = 7), and in biopsies in patients with the diagnosis of EPS (n = 7). Double immunofluorescence was used to co-localize α-smooth-muscle actin (α-SMA) and CD147 in selected biopsies from each group. Expression was scored semi-quantitatively. RESULTS: In biopsies from uremic controls, CD147 was prominently expressed in mesothelial cells, focally between fat cells and by some perivascular cells. In patients on PD, a similar distribution was present (although mesothelium was rarely conserved), with some focal accentuation. In EPS, layers of fibroblastic cells were positive for CD147. EPS biopsies demonstrated a significantly higher score in a blinded evaluation, compared to uremic patients. Cells expressing CD147 were α-SMA positive myofibroblasts as demonstrated by double immunofluorescence. Mean CD147 scores did not differ between patients with different transporter status. CONCLUSIONS: This is the first study demonstrating CD147 on a major part of fibroblastic cells in EPS. Future studies need to address the role of these cells in this severe complication of long-term PD.
Authors: J Tang; Y-S Guo; Y Zhang; X-L Yu; L Li; W Huang; Y Li; B Chen; J-L Jiang; Z-N Chen Journal: Cell Death Differ Date: 2012-05-18 Impact factor: 15.828
Authors: Joerg Latus; Christoph Ulmer; Peter Fritz; Bianka Rettenmaier; Dagmar Biegger; Thomas Lang; German Ott; Christoph Scharpf; Martin Kimmel; Wolfgang Steurer; M Dominik Alscher; Niko Braun Journal: Nephrol Dial Transplant Date: 2012-06-25 Impact factor: 5.992
Authors: Niko Braun; Kontheari Sen; M Dominik Alscher; Peter Fritz; Martin Kimmel; Johann Morelle; Eric Goffin; Achim Jörres; Rudolf P Wüthrich; Clemens D Cohen; Stephan Segerer Journal: Perit Dial Int Date: 2013-02-01 Impact factor: 1.756
Authors: Niko Braun; M Dominik Alscher; Peter Fritz; Joerg Latus; Ilka Edenhofer; Fabian Reimold; Seth L Alper; Martin Kimmel; Dagmar Biegger; Maja Lindenmeyer; Clemens D Cohen; Rudolf P Wüthrich; Stephan Segerer Journal: PLoS One Date: 2012-12-31 Impact factor: 3.240