Chih-Lang Lin1,2,3, Rong-Nan Chien1,3, Kung-Hao Liang2, Po-Yuan Ke2,3, Ya-Hui Huang4, Chau-Ting Yeh4,3. 1. Liver Research Unit, Keelung Chang Gung Memorial Hospital, Keelung, Taiwan, R.O.C. 2. Liver Research Center, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan, R.O.C. 3. Department of Biochemistry and Molecular Biology, Chang Gung University, Taoyuan, Taiwan, R.O.C. 4. Liver Research Center, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan, R.O.C. e1249060@gmail.com chautingy@gmail.com.
Abstract
Background /Aim: Reticulon 3 (RTN3), resides predominantly in the endoplasmic reticulum and has opposite regulatory effects on Hepatitis C virus (HCV) replication through interacting with NS5A and NS4B proteins. This study aimed to unravel the actual effect of RTN3 on HCV replication. MATERIALS AND METHODS: A total of 115 HCV-related hepatocellular carcinoma patients receiving hepatectomy was enrolled in this study. The hepatic HCV RNA and RTN3 protein levels in the non-cancerous liver tissues were examined for clinical analysis. RESULTS: Of the 115 patients, 16 (11.5%) were occult HBV infection (positive for tissue HBV DNA. Univariate followed by multivariate analysis revealed that intrahepatic RTN3 levels were independently associated with higher HCV viral load (p=0.018) and HCV genotype 1 (p=0.017). Multivariate analysis revealed that HCV genotype 1, tumor size, albumin and aspartate transaminase associated with a shorter recurrence-free survival (p<0.05). CONCLUSION: Higher intrahepatic RTN3 levels independently correlated with higher intrahepatic HCV RNA levels and genotype 1 HCV. Copyright
Background /Aim: Reticulon 3 (RTN3), resides predominantly in the endoplasmic reticulum and has opposite regulatory effects on Hepatitis C virus (HCV) replication through interacting with NS5A and NS4B proteins. This study aimed to unravel the actual effect of RTN3 on HCV replication. MATERIALS AND METHODS: A total of 115 HCV-related hepatocellular carcinomapatients receiving hepatectomy was enrolled in this study. The hepatic HCV RNA and RTN3 protein levels in the non-cancerous liver tissues were examined for clinical analysis. RESULTS: Of the 115 patients, 16 (11.5%) were occult HBV infection (positive for tissue HBV DNA. Univariate followed by multivariate analysis revealed that intrahepatic RTN3 levels were independently associated with higher HCV viral load (p=0.018) and HCV genotype 1 (p=0.017). Multivariate analysis revealed that HCV genotype 1, tumor size, albumin and aspartate transaminase associated with a shorter recurrence-free survival (p<0.05). CONCLUSION: Higher intrahepatic RTN3 levels independently correlated with higher intrahepatic HCV RNA levels and genotype 1 HCV. Copyright
Authors: Zhigong Chen; Xinkuan Wang; Xiaodong Hou; Fan Ding; Kang Yi; Peng Zhang; Tao You Journal: J Cardiovasc Transl Res Date: 2020-05-13 Impact factor: 4.132