Quoc Thang Pham1, Naohide Oue2, Yuji Yamamoto1, Yoshinori Shigematsu1,3, Yohei Sekino1,3, Naoya Sakamoto1, Kazuhiro Sentani1, Naohiro Uraoka4, Mamata Tiwari5, Wataru Yasui1. 1. Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan. 2. Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan naoue@hiroshima-u.ac.jp. 3. Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan. 4. Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A. 5. Department of Pathology, Chitwan Medical College Teaching Hospital, Chitwan, Nepal.
Abstract
BACKGROUND: Renal cell carcinoma (RCC) is one of the most common types of cancer in developed countries. Bone marrow stromal cell antigen 2 (BST2) gene, which encodes BST2 transmembrane glycoprotein, is overexpressed in several cancer types. In the present study, we analyzed the expression and function of BST2 in RCC. MATERIALS AND METHODS: BST2 expression was analyzed by immunohistochemistry in 123 RCC cases. RNA interference was used to inhibit BST2 expression in a RCC cell line. RESULTS: Immunohistochemical analysis showed that 32% of the 123 RCC cases were positive for BST2. BST2 expression was positively associated with tumour stage. Furthermore, BST2 expression was an independent predictor of survival in patients with RCC. BST2 siRNA-transfected Caki-1 cells displayed significantly reduced cell growth and invasive activity relative to negative control siRNA-transfected cells. CONCLUSION: These results suggest that BST2 plays an important role in the progression of RCC. Because BST2 is expressed on the cell membrane, BST2 is a good therapeutic target for RCC. Copyright
BACKGROUND:Renal cell carcinoma (RCC) is one of the most common types of cancer in developed countries. Bone marrow stromal cell antigen 2 (BST2) gene, which encodes BST2 transmembrane glycoprotein, is overexpressed in several cancer types. In the present study, we analyzed the expression and function of BST2 in RCC. MATERIALS AND METHODS:BST2 expression was analyzed by immunohistochemistry in 123 RCC cases. RNA interference was used to inhibit BST2 expression in a RCC cell line. RESULTS: Immunohistochemical analysis showed that 32% of the 123 RCC cases were positive for BST2. BST2 expression was positively associated with tumour stage. Furthermore, BST2 expression was an independent predictor of survival in patients with RCC. BST2 siRNA-transfected Caki-1 cells displayed significantly reduced cell growth and invasive activity relative to negative control siRNA-transfected cells. CONCLUSION: These results suggest that BST2 plays an important role in the progression of RCC. Because BST2 is expressed on the cell membrane, BST2 is a good therapeutic target for RCC. Copyright
Authors: Sabrina H Rossi; Izzy Newsham; Sara Pita; Kevin Brennan; Gahee Park; Christopher G Smith; Radoslaw P Lach; Thomas Mitchell; Junfan Huang; Anne Babbage; Anne Y Warren; John T Leppert; Grant D Stewart; Olivier Gevaert; Charles E Massie; Shamith A Samarajiwa Journal: Sci Adv Date: 2022-09-28 Impact factor: 14.957