Muhammad Khalid1, Danial Hassani2, Muhammad Bilal3, Zahid Ali Butt4, Muhammad Hamayun4, Ayaz Ahmad5, Danfeng Huang6, Anwar Hussain7. 1. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Department of Botany, Abdul Wali Khan University, Mardan, Pakistan. 2. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. 3. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. 4. Department of Botany, Abdul Wali Khan University, Mardan, Pakistan. 5. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, China; Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan. 6. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: hdf@sjtu.edu.cn. 7. Department of Botany, Abdul Wali Khan University, Mardan, Pakistan. Electronic address: drhussain@AWKUM.edu.pk.
Abstract
OBJECTIVE: Biofilms are complex, multi-species bacterial communities that colonize the oral cavity in the form of plaque and are known to cause dental caries and periodontal disease. Present study demonstrated the potential of three selected medicinal plants against isolated and identified dental biofilm forming strains. METHODS: Pathogenic bacteria from dental biofilms were isolated, cultured, identified by phylogenetic analysis using PCR-based 16S ribosomal RNA (or 16S rRNA) nucleotide sequences and were analyzed for their biofilm forming capability. The antimicrobial activity of the three important medicinal plant extracts (Acacia arabica, Tamarix aphylla L. and Melia azadirachta L.) was determined against the highest biofilm forming bacteria. RESULTS: Phylogenetic analysis revealed that the 19 strains belonged to Proteobacteria, Firmicutes and Actinobacteria. Among the 19 isolates, eleven strains were found to possess high biofilm formation capability comparatively and antimicrobial activity assay showed that the selected plants considerably inhibited their growth. Extract from A. arabica stem had strong effect on the ability of bacteria isolated from dental carries as evident by up to 73% reduction in biofilm formation on surface pre-treated with extract from this plant. The same extract also showed tremendous biofilm cleaning potential up to 87% of the biofilm. CONCLUSION: The results suggested that the extracts of selected medicinal plants could be used for protection against pathogenic dental biofilm causing bacteria and attempts should be taken by pharmaceutical industries to utilize it in dental caring products.
OBJECTIVE: Biofilms are complex, multi-species bacterial communities that colonize the oral cavity in the form of plaque and are known to cause dental caries and periodontal disease. Present study demonstrated the potential of three selected medicinal plants against isolated and identified dental biofilm forming strains. METHODS: Pathogenic bacteria from dental biofilms were isolated, cultured, identified by phylogenetic analysis using PCR-based 16S ribosomal RNA (or 16S rRNA) nucleotide sequences and were analyzed for their biofilm forming capability. The antimicrobial activity of the three important medicinal plant extracts (Acacia arabica, Tamarix aphylla L. and Melia azadirachta L.) was determined against the highest biofilm forming bacteria. RESULTS: Phylogenetic analysis revealed that the 19 strains belonged to Proteobacteria, Firmicutes and Actinobacteria. Among the 19 isolates, eleven strains were found to possess high biofilm formation capability comparatively and antimicrobial activity assay showed that the selected plants considerably inhibited their growth. Extract from A. arabica stem had strong effect on the ability of bacteria isolated from dental carries as evident by up to 73% reduction in biofilm formation on surface pre-treated with extract from this plant. The same extract also showed tremendous biofilm cleaning potential up to 87% of the biofilm. CONCLUSION: The results suggested that the extracts of selected medicinal plants could be used for protection against pathogenic dental biofilm causing bacteria and attempts should be taken by pharmaceutical industries to utilize it in dental caring products.
Authors: Leilei Zhang; Maha M Ismail; Gabriele Rocchetti; Nesrin M Fayek; Luigi Lucini; Fatema R Saber Journal: Molecules Date: 2022-01-10 Impact factor: 4.411