Wipawee Thoungseabyoun1, Apussara Tachow1, Sawetree Pakkarato1, Atsara Rawangwong1, Suthankamon Krongyut1, Waraporn Sakaew1, Hisatake Kondo2, Wiphawi Hipkaeo3. 1. Nanomorphology-based Apply Research Group & Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, Khon Kaen University, Muang, Khon Kaen, Thailand. 2. Nanomorphology-based Apply Research Group & Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, Khon Kaen University, Muang, Khon Kaen, Thailand; Department of Organ Anatomy, Graduate School of Medicine, Tohoku University, Sendai, Japan. 3. Nanomorphology-based Apply Research Group & Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, Khon Kaen University, Muang, Khon Kaen, Thailand. Electronic address: wiphawi@kku.ac.th.
Abstract
OBJECTIVE: We wished to investigate the subcellular localization of CB1, a receptor for the endocannabinoids in mouse submandibular glands (SMGs) under normal conditions and when stimulated by adrenergic or cholinergic agonists. MATERIALS AND METHODS: SMGs of both male and female adult mice were utilized for immunoblotting and immuno-light and -electron microscopic analyses. Isoproterenol and carbachol were used as adrenergic and cholinergic stimulants, respectively. SMGs were examined at 15, 30, 60 and 120min after intraperitoneal injection of these agents. RESULTS: Selective localization of intense immunoreactivity for CB1 in the granular convoluted ductal cells was confirmed by immunoblotting and the antigen absorption test. In SMGs of control male mice, CB1-immunoreactivity was evident on the basolateral plasma membranes, including the basal infoldings, but was absent on the apical membranes in the ductal cells. Localization and intensity of CB1-immunoreactivity were essentially the same in SMGs of female mice. The immunoreactivity was transiently localized in the apical plasmalemma of some acinar and granular ductal cells of male SMGs shortly after stimulation by isoproterenol, but not by carbachol. CONCLUSION: The present finding suggests that CB1 functions primarily in the basolateral membranes of the granular convoluted ductal cells of SMGs under normal conditions, and that the CB1 can function additionally in the apical membrane of acinar and granular ductal cells for modulation of the saliva secretory condition via adrenoceptors.
OBJECTIVE: We wished to investigate the subcellular localization of CB1, a receptor for the endocannabinoids in mouse submandibular glands (SMGs) under normal conditions and when stimulated by adrenergic or cholinergic agonists. MATERIALS AND METHODS: SMGs of both male and female adult mice were utilized for immunoblotting and immuno-light and -electron microscopic analyses. Isoproterenol and carbachol were used as adrenergic and cholinergic stimulants, respectively. SMGs were examined at 15, 30, 60 and 120min after intraperitoneal injection of these agents. RESULTS: Selective localization of intense immunoreactivity for CB1 in the granular convoluted ductal cells was confirmed by immunoblotting and the antigen absorption test. In SMGs of control male mice, CB1-immunoreactivity was evident on the basolateral plasma membranes, including the basal infoldings, but was absent on the apical membranes in the ductal cells. Localization and intensity of CB1-immunoreactivity were essentially the same in SMGs of female mice. The immunoreactivity was transiently localized in the apical plasmalemma of some acinar and granular ductal cells of male SMGs shortly after stimulation by isoproterenol, but not by carbachol. CONCLUSION: The present finding suggests that CB1 functions primarily in the basolateral membranes of the granular convoluted ductal cells of SMGs under normal conditions, and that the CB1 can function additionally in the apical membrane of acinar and granular ductal cells for modulation of the saliva secretory condition via adrenoceptors.
Authors: Samar A Alghamdi; Maryam H Mugri; Nahid M H Elamin; Mona Awad Kamil; Hind Osman; Basma G Eid; Rasheed A Shaik; Soad S Shaker; Aziza Alrafiah Journal: Curr Issues Mol Biol Date: 2022-05-27 Impact factor: 2.976