Literature DB >> 2854459

Studies on the immobilization of glucuronidase (Part 1). Covalent immobilization on various carriers (a comparison).

E Rapatz1, A Travnicek, G Fellhofer, F Pittner.   

Abstract

beta-Glucuronidase (EC 3.2.1.31) was immobilized on various organic and inorganic carriers by different methods. Optimum coupling conditions have been worked out. The immobilization were characterized and compared to each other. Parameters resulting in most stable preparations with high activities are discussed.

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Year:  1988        PMID： 2854459     DOI： 10.1007/bf02921494

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  7 in total

1.  Immobilised enxymes.

Authors:  K Mosbach
Journal:  FEBS Lett       Date:  1976-02-04       Impact factor: 4.124

2.  Determination of nitrogen in proteins by means of idanetrione hydrate.

Authors:  S JACOBS
Journal:  Nature       Date:  1959-01-24       Impact factor: 49.962

3.  Application of a Theory of Enzyme Specificity to Protein Synthesis.

Authors:  D E Koshland
Journal:  Proc Natl Acad Sci U S A       Date:  1958-02       Impact factor: 11.205

4.  Cooperativity and the slow isomerization of deta-chymotrypsin.

Authors:  G R Ainslie; K E Neet
Journal:  Mol Cell Biochem       Date:  1979-04-02       Impact factor: 3.396

5.  The derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbents.

Authors:  J K Inman; H M Dintzis
Journal:  Biochemistry       Date:  1969-10       Impact factor: 3.162

6.  Chick embryo liver beta-glucuronidase. Comparison of activity on natural and artificial substrates.

Authors:  J H Glaser; H E Conrad
Journal:  J Biol Chem       Date:  1979-07-25       Impact factor: 5.157

7.  Beta-Glucuronidase. II. Purification of calf liver beta-glucuronidase.

Authors:  P BERNFELD; J S NISSELBAUM; W H FISHMAN
Journal:  J Biol Chem       Date:  1953-06       Impact factor: 5.157
  7 in total
  1 in total

1.  Studies on the immobilization of glucuronidase (Part 2). Cleavage of hardly soluble substrates in organic solvents.

Authors:  E Rapatz; M Ambros; B Kopp; F Pittner
Journal:  Appl Biochem Biotechnol       Date:  1988-12       Impact factor: 2.926
  1 in total

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