Elizabeth Boaden1, Mary Lyons1, Sim K Singhrao2, Hazel Dickinson1, Michael Leathley1, Catherine E Lightbody1, Alison McLoughlin1, Zarine Khan2, Stjohn Crean2, Craig Smith3, Susan Higham4, Caroline Watkins1,5. 1. Faculty of Health and Wellbeing, School of Nursing, University of Central Lancashire, Preston, UK. 2. Dementia & Neurodegenerative Diseases Research Group, College of Clinical and Biomedical Sciences, School of Dentistry, University of Central Lancashire, Preston, UK. 3. Greater Manchester Comprehensive Stroke Centre, and University of Manchester Stroke and Vascular Centre, Manchester Academic Health Science Centre, Salford Royal NHS Foundation Trust, Salford, UK. 4. Department of Health Services Research, Institute of Psychology, Health and Society, The Research Wing, School of Dentistry, University of Liverpool, Liverpool, UK. 5. Australian Catholic University, North Sydney, Australia.
Abstract
OBJECTIVE: To describe the bacterial profile of the oral flora during the first 2 weeks following a stroke, examining changes in the condition of the oral cavity and infections. BACKGROUND: Dysphagia is common after a stroke and can lead to aspiration pneumonia. Oral flora changes associated with stroke have been implicated as a possible source of bacteria that can cause systemic infections. MATERIALS AND METHODS: Seventy-seven participants were recruited over a period of 9 months. Fifty participants had a complete set of swabs from four different oral sites and a saliva sample taken at three time points over a 14-day period. Molecular identification of bacteria was performed on the pooled DNA extracted. RESULTS: A total of 103 bacterial phylotypes were identified, 29 of which were not in the Human Oral Microbiome Database (HOMD). Fourteen of the twenty most common bacterial phylotypes found in the oral cavity were Streptococcal species with Streptococcus salivarius being the most common. The condition of the oral cavity worsened during the study period. Fifteen (30%) patients had at least one infection. CONCLUSIONS: There appears to be huge diversity of bacterial organisms in the oral cavity of stroke patients, and as most phylotypes identified were only found in one or two participants, no particular patterns linked to infection or the condition of the oral cavity could be discerned.
OBJECTIVE: To describe the bacterial profile of the oral flora during the first 2 weeks following a stroke, examining changes in the condition of the oral cavity and infections. BACKGROUND:Dysphagia is common after a stroke and can lead to aspiration pneumonia. Oral flora changes associated with stroke have been implicated as a possible source of bacteria that can cause systemic infections. MATERIALS AND METHODS: Seventy-seven participants were recruited over a period of 9 months. Fifty participants had a complete set of swabs from four different oral sites and a saliva sample taken at three time points over a 14-day period. Molecular identification of bacteria was performed on the pooled DNA extracted. RESULTS: A total of 103 bacterial phylotypes were identified, 29 of which were not in the Human Oral Microbiome Database (HOMD). Fourteen of the twenty most common bacterial phylotypes found in the oral cavity were Streptococcal species with Streptococcus salivarius being the most common. The condition of the oral cavity worsened during the study period. Fifteen (30%) patients had at least one infection. CONCLUSIONS: There appears to be huge diversity of bacterial organisms in the oral cavity of strokepatients, and as most phylotypes identified were only found in one or two participants, no particular patterns linked to infection or the condition of the oral cavity could be discerned.
Authors: Anne I Boullerne; Guy R Adami; Joel L Schwartz; Demetrios Skias; Mark Maienschein-Cline; Stefan J Green; Douglas L Feinstein Journal: J Neuroimmunol Date: 2020-04-07 Impact factor: 3.478
Authors: Mary Lyons; Craig Smith; Elizabeth Boaden; Marian C Brady; Paul Brocklehurst; Hazel Dickinson; Shaheen Hamdy; Susan Higham; Peter Langhorne; Catherine Lightbody; Giles McCracken; Antonieta Medina-Lara; Lise Sproson; Angus Walls; Dame Caroline Watkins Journal: Eur Stroke J Date: 2018-05-08