| Literature DB >> 28535442 |
Frits F B Hulshof1, Bernke Papenburg2, Aliaksei Vasilevich3, Marc Hulsman4, Yiping Zhao2, Marloes Levers2, Natalie Fekete2, Meint de Boer5, Huipin Yuan6, Shantanu Singh7, Nick Beijer3, Mark-Anthony Bray7, David J Logan7, Marcel Reinders4, Anne E Carpenter7, Clemens van Blitterswijk8, Dimitrios Stamatialis9, Jan de Boer10.
Abstract
Stem cells respond to the physicochemical parameters of the substrate on which they grow. Quantitative material activity relationships - the relationships between substrate parameters and the phenotypes they induce - have so far poorly predicted the success of bioactive implant surfaces. In this report, we screened a library of randomly selected designed surface topographies for those inducing osteogenic differentiation of bone marrow-derived mesenchymal stem cells. Cell shape features, surface design parameters, and osteogenic marker expression were strongly correlated in vitro. Furthermore, the surfaces with the highest osteogenic potential in vitro also demonstrated their osteogenic effect in vivo: these indeed strongly enhanced bone bonding in a rabbit femur model. Our work shows that by giving stem cells specific physicochemical parameters through designed surface topographies, differentiation of these cells can be dictated.Entities:
Keywords: Bone implants; Computational modeling; Differentiation; High-throughput screening; Micro-fabrication; Surface topography
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Year: 2017 PMID： 28535442 DOI： 10.1016/j.biomaterials.2017.05.020
Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479