| Literature DB >> 28535353 |
Yaqiong Zhang, Lanteng Wang, Xing Shen, Xiaoqun Wei, Xinan Huang1, Yingju Liu, Xiulan Sun2, Zhanhui Wang3, Yuanming Sun, Zhenlin Xu, Sergei A Eremin4,5, Hongtao Lei.
Abstract
Ochratoxins A, B, and C (OTA, OTB, and OTC) can be found in cereals and feeds; the simultaneous detection of these ochratoxins holds a great need in food safety. In this study, four antibodies raised from two ochrotoxin haptens and two coating antigens were compared, and then a sensitive and broad-specificity enzyme-linked immunosorbent assay (ELISA) was established for the simultaneous determination of three ochratoxins, where the detection limits were 0.005, 0.001, and 0.001 ng/mL for OTA, OTB, and OTC, respectively, and recoveries of three ochratoxins were between 84.3% and 111.7%. This developed method had been successfully applied to detect ochratoxins in both millet and maize. Molecular modeling revealed that the broad-specificity was related with the chlorine electronegativity on OTA and OTC and the potential of the acetyl ester group on OTC. The proposed ELISA can be used for simultaneous detection of three ochratoxins.Entities:
Keywords: antibody; broad-specificity; immunoassay; molecular modeling; ochratoxin
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Year: 2017 PMID： 28535353 DOI： 10.1021/acs.jafc.7b00770
Source DB: PubMed Journal: J Agric Food Chem ISSN： 0021-8561 Impact factor: 5.279