S Geravandi1, M Azadbakht2. 1. Department of Biology, Faculty of Basic Sciences, Razi University, Kermanshah, Iran. 2. Department of Biology, Faculty of Basic Sciences, Razi University, Kermanshah, Iran. azadbakhtm_tmu@yahoo.com.
Abstract
BACKGROUND: Cryopreservation of ovary is a relevant option for preserving fertility of cancer patients undergoing chemotherapy. OBJECTIVE: To evaluate the effect of zinc in the vitrification medium on histology and follicle growth. MATERIALS AND METHODS: Mouse ovaries were vitrified in vitrification medium supplemented with 0, 100, 150 or 200 μg/dl zinc, identified as V0, V1, V2 and V3 groups, respectively. Histological evaluation of ovaries was carried out. The isolated pre-antral follicles were cultured. The size and growth of follicles were assessed. RESULTS: The percentage of morphologically normal follicles increased with increasing zinc concentration in the vitrification medium (P < 0.05). Follicle viability was higher in the V3 than V0 group at the beginning and end of culture (P < 0.05). The highest follicle diameter was obtained in the V3 group after 6 days of culture (P < 0.05). CONCLUSION: Supplementation of the vitrification medium with zinc can improve follicle viability and growth.
BACKGROUND: Cryopreservation of ovary is a relevant option for preserving fertility of cancerpatients undergoing chemotherapy. OBJECTIVE: To evaluate the effect of zinc in the vitrification medium on histology and follicle growth. MATERIALS AND METHODS:Mouseovaries were vitrified in vitrification medium supplemented with 0, 100, 150 or 200 μg/dl zinc, identified as V0, V1, V2 and V3 groups, respectively. Histological evaluation of ovaries was carried out. The isolated pre-antral follicles were cultured. The size and growth of follicles were assessed. RESULTS: The percentage of morphologically normal follicles increased with increasing zinc concentration in the vitrification medium (P < 0.05). Follicle viability was higher in the V3 than V0 group at the beginning and end of culture (P < 0.05). The highest follicle diameter was obtained in the V3 group after 6 days of culture (P < 0.05). CONCLUSION: Supplementation of the vitrification medium with zinc can improve follicle viability and growth.
Authors: George A Perry; Stephanie D Perkins; Emmalee J Northrop; Jerica J J Rich; Kaitlin M Epperson; Taylor N Andrews; Adalaide C Kline; Lacey K Quail; Julie A Walker; Cody L Wright; Jason R Russell Journal: J Anim Sci Date: 2021-07-01 Impact factor: 3.159