Yangcai Wang1,2, Bailin Chen1,3, Tao Lin1,2, Shengde Wu1,4, Guanghui Wei1,4. 1. a Department of Urology , Children's Hospital of Chongqing Medical University , Chongqing , China. 2. b Key Laboratory of Pediatrics in Chongqing , Chongqing , China. 3. c Ministry of Education Key Laboratory of Child Development and Disorders , Chongqing , China. 4. d Chongqing International Science and Technology Cooperation Center for Child Development and Disorders , Chongqing , China.
Abstract
OBJECTIVE: To investigate the protective/ameliorative effects of vitamin E on di-2-(ethylhexyl) phthalate (DEHP)-induced reproductive toxicity, particularly in testicular toxicity in male rats, emphasizing peroxisome proliferator-activated receptor (PPAR)-dependent mechanism. METHODS: Sprague-Dawley females were exposed by oral route to DEHP alone or associated with vitamin E from gestation day (GD) 12.5 to postnatal day (PND) 3 according to the following treatment regimens: vehicle control (corn oil), vitamin E (200 mg/kg)+corn oil, DEHP (500 mg/kg)+corn oil, and DEHP (500 mg/kg)+vitamin E (200 mg/kg)+corn oil. Variables including litter size, sex ratio, pup weight, post-implantation losses, and the number of viable pups were also assessed. Three male pups per litter were randomly selected and necropsied to measure paired testes weight, apoptosis, and gene expression on PND 3. To evaluate the long-term protective effects of vitamin E, three randomly selected males were necropsied to measure testis histology on PND 70. RESULTS: Supplementation of vitamin E (200 mg/kg) reduced malformations, increased testes weight and prevented the maternal bodyweight loss induced by DEHP. Litter size, sex ratio, and number of viable pups were unaffected, but vitamin E co-administration declined testicular cell apoptosis, decreased the PPARs expression, and protected testis histology. CONCLUSIONS: Vitamin E cotreatment showed protective effects against DEHP-induced testicular toxicity, including reproductive malformations, testicular weight, apoptosis and histology, and the mechanisms maybe associated with PPARs.
OBJECTIVE: To investigate the protective/ameliorative effects of vitamin E on di-2-(ethylhexyl) phthalate (DEHP)-induced reproductive toxicity, particularly in testicular toxicity in male rats, emphasizing peroxisome proliferator-activated receptor (PPAR)-dependent mechanism. METHODS: Sprague-Dawley females were exposed by oral route to DEHP alone or associated with vitamin E from gestation day (GD) 12.5 to postnatal day (PND) 3 according to the following treatment regimens: vehicle control (corn oil), vitamin E (200 mg/kg)+corn oil, DEHP (500 mg/kg)+corn oil, and DEHP (500 mg/kg)+vitamin E (200 mg/kg)+corn oil. Variables including litter size, sex ratio, pup weight, post-implantation losses, and the number of viable pups were also assessed. Three male pups per litter were randomly selected and necropsied to measure paired testes weight, apoptosis, and gene expression on PND 3. To evaluate the long-term protective effects of vitamin E, three randomly selected males were necropsied to measure testis histology on PND 70. RESULTS: Supplementation of vitamin E (200 mg/kg) reduced malformations, increased testes weight and prevented the maternal bodyweight loss induced by DEHP. Litter size, sex ratio, and number of viable pups were unaffected, but vitamin E co-administration declined testicular cell apoptosis, decreased the PPARs expression, and protected testis histology. CONCLUSIONS:Vitamin E cotreatment showed protective effects against DEHP-induced testicular toxicity, including reproductive malformations, testicular weight, apoptosis and histology, and the mechanisms maybe associated with PPARs.
Authors: Kari Neier; Luke Montrose; Kathleen Chen; Maureen A Malloy; Tamara R Jones; Laurie K Svoboda; Craig Harris; Peter X K Song; Subramaniam Pennathur; Maureen A Sartor; Dana C Dolinoy Journal: Environ Epigenet Date: 2020-12-23
Authors: Ines Amara; Maria Laura Ontario; Maria Scuto; Gianluigi Maria Lo Dico; Sebastiano Sciuto; Valentina Greco; Salwa Abid-Essefi; Anna Signorile; Angela Trovato Salinaro; Vittorio Calabrese Journal: Antioxidants (Basel) Date: 2021-03-29