Donatus W Adongo1, Priscilla K Mante2, Kennedy K E Kukuia3, Robert P Biney4, Eric Boakye-Gyasi5, Charles K Benneh6, Elvis O Ameyaw7, Eric Woode8. 1. Department of Pharmacology, School of Medicine, University of Health and Allied Sciences, Ho, Ghana. Electronic address: donatusadongo@yahoo.com. 2. Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. Electronic address: pkmante.chs@knust.edu.gh. 3. Department of Pharmacology and Toxicology, University of Ghana School of Pharmacy, University of Ghana, Accra, Ghana. Electronic address: edemkennedy@yahoo.com. 4. Department of Pharmacology, School of Medical Sciences, University of Cape Coast, Cape Coast, Ghana. Electronic address: rpbiney@hotmail.com. 5. Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. Electronic address: ebgyasi.pharm@knust.edu.gh. 6. Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. Electronic address: benneh.ck@outlook.com. 7. Department of Biomedical and Forensic Sciences, School of Biological Science, University of Cape Coast, Cape Coast, Ghana. Electronic address: elvisameyaw@gmail.com. 8. Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. Electronic address: ewoode.pharm@knust.edu.gh.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Pseudospondias microcarpa (A. Rich) Engl. is a plant used for managing various diseases including central nervous system disorders. AIM OF THE STUDY: This study explored the anticonvulsant activity of P. microcarpa hydroethanolic leaf extract (PME) as well as possible mechanism(s) of action in animal models. METHODS: Effects of PME was assessed in electroconvulsive (the maximal electroshock and 6-Hz seizures) and chemoconvulsive (pentylenetetrazole-, picrotoxin-, isoniazid-, 4-aminopyridine-, and strychnine-induced seizures) models of epilepsy. In addition, effect of the extract on the nitric oxide pathway and GABAA receptor complex was evaluated. RESULTS: The extract (30, 100 and 300mgkg-1, p.o.) significantly delayed the onset as well as decreased the duration and frequency of pentylenetetrazole-, picrotoxin- and strychnine-induced seizures. In addition, PME pre-treatment significantly improved survival in the 4-aminopyridine- and isoniazid-induced seizure tests. Furthermore, the extract protected against 6-Hz psychomotor seizures but had no effect in the maximal electroshock test. The anticonvulsant effect of PME (100mgkg-1, p.o.) was also reversed by pre-treatment with flumazenil, L-arginine or sildenafil. However, L-NAME or methylene blue (MB) augmented its effect. CONCLUSION: Results show that PME has anticonvulsant activity and may probably be affecting GABAergic, glycinergic, NMDA, K+ channels and nitric oxide-cGMP pathways to exert its effect.
ETHNOPHARMACOLOGICAL RELEVANCE: Pseudospondias microcarpa (A. Rich) Engl. is a plant used for managing various diseases including central nervous system disorders. AIM OF THE STUDY: This study explored the anticonvulsant activity of P. microcarpahydroethanolic leaf extract (PME) as well as possible mechanism(s) of action in animal models. METHODS: Effects of PME was assessed in electroconvulsive (the maximal electroshock and 6-Hz seizures) and chemoconvulsive (pentylenetetrazole-, picrotoxin-, isoniazid-, 4-aminopyridine-, and strychnine-induced seizures) models of epilepsy. In addition, effect of the extract on the nitric oxide pathway and GABAA receptor complex was evaluated. RESULTS: The extract (30, 100 and 300mgkg-1, p.o.) significantly delayed the onset as well as decreased the duration and frequency of pentylenetetrazole-, picrotoxin- and strychnine-induced seizures. In addition, PME pre-treatment significantly improved survival in the 4-aminopyridine- and isoniazid-induced seizure tests. Furthermore, the extract protected against 6-Hz psychomotor seizures but had no effect in the maximal electroshock test. The anticonvulsant effect of PME (100mgkg-1, p.o.) was also reversed by pre-treatment with flumazenil, L-arginine or sildenafil. However, L-NAME or methylene blue (MB) augmented its effect. CONCLUSION: Results show that PME has anticonvulsant activity and may probably be affecting GABAergic, glycinergic, NMDA, K+ channels and nitric oxide-cGMP pathways to exert its effect.
Authors: Donatus Wewura Adongo; Priscilla Kolibea Mante; Kennedy Kwami Edem Kukuia; Charles Kwaku Benneh; Robert Peter Biney; Eric Boakye-Gyasi; Nicholas Akinwale Titiloye; Eric Woode Journal: ScientificWorldJournal Date: 2018-05-20
Authors: Charles Kwaku Benneh; Robert Peter Biney; Augustine Tandoh; Felix Agyei Ampadu; Donatus Wewura Adongo; Jonathan Jato; Eric Woode Journal: Evid Based Complement Alternat Med Date: 2018-05-02 Impact factor: 2.629
Authors: Charles Kwaku Benneh; Robert Peter Biney; Donatus Wewura Adongo; Priscilla Kolibea Mante; Felix Agyei Ampadu; Augustine Tandoh; Jonathan Jato; Eric Woode Journal: Depress Res Treat Date: 2018-09-09
Authors: Ernest Obese; Robert Peter Biney; Isaac Tabiri Henneh; Emmanuel Awintiig Adakudugu; Daniel Anokwah; Lovia Serwaa Agyemang; Eric Woode; Elvis Ofori Ameyaw Journal: Neural Plast Date: 2021-06-26 Impact factor: 3.599