Masamichi Sato1, Tetsuo Shoda2, Hirotaka Shimizu3, Kanami Orihara4, Kyoko Futamura5, Akio Matsuda5, Yoshiyuki Yamada6, Rie Irie7, Takako Yoshioka7, Toshiaki Shimizu8, Yukihiro Ohya9, Ichiro Nomura10, Kenji Matsumoto5, Katsuhiro Arai3. 1. Division of Gastroenterology, National Center for Child Health and Development, Tokyo, Japan; Department of Pediatrics, Juntendo University Graduate School of Medicine, Tokyo, Japan. 2. Department of Allergy and Clinical Immunology, National Research Institute for Child Health and Development, Tokyo, Japan; Division of Allergy, National Center for Child Health and Development, Tokyo, Japan. Electronic address: shoda-t@ncchd.go.jp. 3. Division of Gastroenterology, National Center for Child Health and Development, Tokyo, Japan. 4. Department of Allergy and Clinical Immunology, National Research Institute for Child Health and Development, Tokyo, Japan; Waseda Institute for Advanced Study, Waseda University, Tokyo, Japan. 5. Department of Allergy and Clinical Immunology, National Research Institute for Child Health and Development, Tokyo, Japan. 6. Division of Allergy and Immunology, Gunma Children's Medical Center, Gunma, Japan. 7. Department of Pathology, National Center for Child Health and Development, Tokyo, Japan. 8. Department of Pediatrics, Juntendo University Graduate School of Medicine, Tokyo, Japan. 9. Division of Allergy, National Center for Child Health and Development, Tokyo, Japan. 10. Department of Allergy and Clinical Immunology, National Research Institute for Child Health and Development, Tokyo, Japan; Division of Allergy, National Center for Child Health and Development, Tokyo, Japan.
Abstract
BACKGROUND: Eosinophilic gastritis (EG) is clinicopathologically characterized by both marked gastric eosinophilia and clinical symptoms. The endoscopic findings in EG vary among patients, leading to clinical confusion. However, little is known about the relationship between precise endoscopic findings and the pathophysiological process responsible for EG. OBJECTIVE: We aimed to elucidate whether the gross endoscopic findings of EG can be classified into distinct gene expression profiles. METHODS: We enrolled pediatric patients who underwent gastrointestinal endoscopy for clinical symptoms suggestive of eosinophilic gastrointestinal disorder between 2011 and 2016. EG was diagnosed when gastric eosinophilia was greater than or equal to 30 eosinophils/hpf. The gene expression profiles of gastric biopsies were assessed using microarray technology. RESULTS: Patients with EG and control subjects (n = 8, each) were examined. On the microarray, 1,999 genes were differentially expressed between EG and the controls (≥2-fold difference, adjusted P value < .05), including significant upregulation of eotaxin-3 (C-C chemokine ligand 26). The endoscopic findings of patients with EG fell roughly into 2 types, namely, ulcerative and nodular lesions. Despite identifying distinct patterns of gene expression, most differentially regulated genes overlapped between the 2 endoscopic finding types. Several gene ontology terms were enriched in the substantially overlapped genes, but not in each of the distinct genes. CONCLUSIONS: Our results strongly indicate that ulcerative and nodular lesions are a single disease, EG, or a variation thereof, in spite of morphological differences. Our findings may contribute to a better understanding of the pathogenesis of EG, as well as to more accurate diagnosis of this disease.
BACKGROUND:Eosinophilic gastritis (EG) is clinicopathologically characterized by both marked gastric eosinophilia and clinical symptoms. The endoscopic findings in EG vary among patients, leading to clinical confusion. However, little is known about the relationship between precise endoscopic findings and the pathophysiological process responsible for EG. OBJECTIVE: We aimed to elucidate whether the gross endoscopic findings of EG can be classified into distinct gene expression profiles. METHODS: We enrolled pediatric patients who underwent gastrointestinal endoscopy for clinical symptoms suggestive of eosinophilic gastrointestinal disorder between 2011 and 2016. EG was diagnosed when gastric eosinophilia was greater than or equal to 30 eosinophils/hpf. The gene expression profiles of gastric biopsies were assessed using microarray technology. RESULTS:Patients with EG and control subjects (n = 8, each) were examined. On the microarray, 1,999 genes were differentially expressed between EG and the controls (≥2-fold difference, adjusted P value < .05), including significant upregulation of eotaxin-3 (C-C chemokine ligand 26). The endoscopic findings of patients with EG fell roughly into 2 types, namely, ulcerative and nodular lesions. Despite identifying distinct patterns of gene expression, most differentially regulated genes overlapped between the 2 endoscopic finding types. Several gene ontology terms were enriched in the substantially overlapped genes, but not in each of the distinct genes. CONCLUSIONS: Our results strongly indicate that ulcerative and nodular lesions are a single disease, EG, or a variation thereof, in spite of morphological differences. Our findings may contribute to a better understanding of the pathogenesis of EG, as well as to more accurate diagnosis of this disease.
Authors: Tetsuo Shoda; Ting Wen; Julie M Caldwell; Margaret H Collins; John A Besse; Garrett A Osswald; J Pablo Abonia; Nicoleta C Arva; Dan Atkins; Kelley E Capocelli; Evan S Dellon; Gary W Falk; Nirmala Gonsalves; Sandeep K Gupta; Ikuo Hirano; Vincent A Mukkada; Philip E Putnam; Rachel M Sheridan; Amanda K Rudman Spergel; Jonathan M Spergel; Joshua B Wechsler; Guang-Yu Yang; Seema S Aceves; Glenn T Furuta; Marc E Rothenberg Journal: J Allergy Clin Immunol Date: 2019-11-16 Impact factor: 10.793
Authors: Tetsuo Shoda; Margaret H Collins; Mark Rochman; Ting Wen; Julie M Caldwell; Lydia E Mack; Garrett A Osswald; John A Besse; Yael Haberman; Seema S Aceves; Nicoleta C Arva; Kelley E Capocelli; Mirna Chehade; Carla M Davis; Evan S Dellon; Gary W Falk; Nirmala Gonsalves; Sandeep K Gupta; Ikuo Hirano; Paneez Khoury; Amy Klion; Calies Menard-Katcher; John Leung; Vincent A Mukkada; Philip E Putnam; Jonathan M Spergel; Joshua B Wechsler; Guang-Yu Yang; Glenn T Furuta; Lee A Denson; Marc E Rothenberg Journal: Gastroenterology Date: 2022-01-25 Impact factor: 33.883
Authors: Ikuo Hirano; Margaret H Collins; Eileen King; Qin Sun; Mirna Chehade; J Pablo Abonia; Peter A Bonis; Kelly E Capocelli; Evan S Dellon; Gary W Falk; Nirmala Gonsalves; Sandeep K Gupta; John Leung; David Katzka; Paul Menard-Katcher; Paneez Khoury; Amy Klion; Vincent A Mukkada; Kathryn Peterson; Tetsuo Shoda; Amanda K Rudman-Spergel; Jonathan A Spergel; Guang-Yu Yang; Marc E Rothenberg; Seema S Aceves; Glenn T Furuta Journal: Am J Gastroenterol Date: 2022-03-01 Impact factor: 12.045