Literature DB >> 28525773

Acetone facilitated DNA sampling from electrical tapes improves DNA recovery and enables latent fingerprints development.

Ilan Feine1, Moshe Shpitzen2, Boris Geller3, Eran Salmon4, Tsach Peleg5, Jonathan Roth6, Ron Gafny7.   

Abstract

Electrical tapes (ETs) are a common component of improvised explosive devices (IEDs) used by terrorists or criminal organizations and represent a valuable forensic resource for DNA and latent fingerprints recovery. However, DNA recovery rates are typically low and usually below the minimal amount required for amplification. In addition, most DNA extraction methods are destructive and do not allow further latent fingerprints development. In the present study a cell culture based touch DNA model was used to demonstrate a two-step acetone-water DNA recovery protocol from ETs. This protocol involves only the adhesive side of the ET and increases DNA recovery rates by up to 70%. In addition, we demonstrated partially successful latent fingerprints development from the non-sticky side of the ETs. Taken together, this protocol maximizes the forensic examination of ETs and is recommended for routine casework processing.
Copyright © 2017 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Adhesive tape; DNA recovery; Electrical tape; Improvised explosive devices (IEDs); Latent fingerprints development; Touch DNA

Mesh:

Substances:

Year:  2017        PMID: 28525773     DOI: 10.1016/j.forsciint.2017.04.023

Source DB:  PubMed          Journal:  Forensic Sci Int        ISSN: 0379-0738            Impact factor:   2.395


  2 in total

Review 1.  Interpol review of fingermarks and other body impressions 2016-2019.

Authors:  Andy Bécue; Heidi Eldridge; Christophe Champod
Journal:  Forensic Sci Int       Date:  2020-03-17       Impact factor: 2.395

2.  Survival of forensic trace evidence on improvised explosive devices: perspectives on individualisation.

Authors:  Natasja Vanderheyden; Elke Verhoeven; Steve Vermeulen; Bram Bekaert
Journal:  Sci Rep       Date:  2020-07-30       Impact factor: 4.379

  2 in total

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