Patrick V Almeida1, Mohammad-Ali Shahbazi1,2, Alexandra Correia1, Ermei Mäkilä1,3, Marianna Kemell4, Jarno Salonen3, Jouni Hirvonen1, Hélder A Santos1,5. 1. Division of Pharmaceutical Chemistry & Technology, Drug Research Program, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5 E (P.O. Box 56), FI-00014 Finland. 2. Department of Micro- & Nanotechnology, Technical University of Denmark, 2800 KGs. Lyngby, Denmark. 3. Laboratory of Industrial Physics, Department of Physics & Astronomy, University of Turku, FI-20014 Finland. 4. Laboratory of Inorganic Chemistry, Department of Chemistry, University of Helsinki, A.I. Virtasen aukio 1 (P.O. Box 55), FI-00014 Finland. 5. Helsinki Institute of Life Science, HiLIFE, University of Helsinki, FI-00014Helsinki, Finland.
Abstract
AIM: To evaluate the chemotherapeutic potential of a novel multifunctional nanocomposite encapsulating both porous silicon (PSi) and gold (Au) nanoparticles in a polymeric nanocomplex. MATERIALS & METHODS: The nanocomposite was physicochemically characterized and evaluated in vitro for biocompatibility, cellular internalization, endosomolytic properties, cytoplasmatic drug delivery and chemotherapeutic efficacy. RESULTS: The nanocomposites were successfully produced and exhibited adequate physicochemical properties and superior in vitro cyto- and hemocompatibilities. The encapsulation of PSi nanoparticles in the nanocomplexes significantly enhanced their cellular internalization and enabled their endosomal escape, resulting in the efficient cytoplasmic delivery of these nanosystems. Sorafenib-loaded nanocomposites showed a potent in vitro antiproliferative effect on MDA-MB-231 breast cancer cells. CONCLUSION: The multifunctional nanocomposite herein presented exhibits great potential as a chemotherapeutic nanoplatform.
AIM: To evaluate the chemotherapeutic potential of a novel multifunctional nanocomposite encapsulating both porous silicon (PSi) and gold (Au) nanoparticles in a polymeric nanocomplex. MATERIALS & METHODS: The nanocomposite was physicochemically characterized and evaluated in vitro for biocompatibility, cellular internalization, endosomolytic properties, cytoplasmatic drug delivery and chemotherapeutic efficacy. RESULTS: The nanocomposites were successfully produced and exhibited adequate physicochemical properties and superior in vitro cyto- and hemocompatibilities. The encapsulation of PSi nanoparticles in the nanocomplexes significantly enhanced their cellular internalization and enabled their endosomal escape, resulting in the efficient cytoplasmic delivery of these nanosystems. Sorafenib-loaded nanocomposites showed a potent in vitro antiproliferative effect on MDA-MB-231 breast cancer cells. CONCLUSION: The multifunctional nanocomposite herein presented exhibits great potential as a chemotherapeutic nanoplatform.
Authors: Patrícia Figueiredo; Mika H Sipponen; Kalle Lintinen; Alexandra Correia; Alexandros Kiriazis; Jari Yli-Kauhaluoma; Monika Österberg; Anne George; Jouni Hirvonen; Mauri A Kostiainen; Hélder A Santos Journal: Small Date: 2019-05-06 Impact factor: 13.281
Authors: Stanislav V Zabotnov; Anastasiia V Skobelkina; Ekaterina A Sergeeva; Daria A Kurakina; Aleksandr V Khilov; Fedor V Kashaev; Tatyana P Kaminskaya; Denis E Presnov; Pavel D Agrba; Dmitrii V Shuleiko; Pavel K Kashkarov; Leonid A Golovan; Mikhail Yu Kirillin Journal: Sensors (Basel) Date: 2020-08-28 Impact factor: 3.576