Ekene Onwuka1,2, Cameron Best1, Andrew Sawyer3,4, Tai Yi1, Eric Heuer1, Malik Sams1, Matthew Wiet1, Hong Zheng1, Themis Kyriakides3,4, Christopher Breuer1,2,5. 1. Tissue Engineering & Surgical Research, The Research Institute at Nationwide Children's Hospital, Columbus, OH, USA. 2. Department of Surgery, The Ohio State University Wexner Medical Center, Columbus, OH, USA. 3. Vascular Biology & Therapeutics, Yale School of Medicine, New Haven, CT, USA. 4. Department of Pathology, Yale School of Medicine, New Haven, CT, USA. 5. Department of Pediatric Surgery, Nationwide Children's Hospital, Columbus, OH, USA.
Abstract
AIM: Inflammatory myeloid lineage cells mediate neotissue formation in tissue-engineered vascular grafts, but the molecular mechanism is not completely understood. We examined the role of vasculogenic PDGF-B in tissue-engineered vascular graft neotissue development. MATERIALS & METHODS: Myeloid cell-specific PDGF-B knockout mice (PDGF-KO) were generated using bone marrow transplantation, and scaffolds were implanted as inferior vena cava interposition grafts in either PDGF-KO or wild-type mice. RESULTS: After 2 weeks, grafts from PDGF-KO mice had more remaining scaffold polymer and less intimal neotissue development. Increased macrophage apoptosis, decreased smooth muscle cell proliferation and decreased collagen content was also observed. CONCLUSION: Myeloid cell-derived PDGF contributes to vascular neotissue formation by regulating macrophage apoptosis, smooth muscle cell proliferation and extracellular matrix deposition.
AIM: Inflammatory myeloid lineage cells mediate neotissue formation in tissue-engineered vascular grafts, but the molecular mechanism is not completely understood. We examined the role of vasculogenic PDGF-B in tissue-engineered vascular graft neotissue development. MATERIALS & METHODS: Myeloid cell-specific PDGF-B knockout mice (PDGF-KO) were generated using bone marrow transplantation, and scaffolds were implanted as inferior vena cava interposition grafts in either PDGF-KO or wild-type mice. RESULTS: After 2 weeks, grafts from PDGF-KO mice had more remaining scaffold polymer and less intimal neotissue development. Increased macrophage apoptosis, decreased smooth muscle cell proliferation and decreased collagen content was also observed. CONCLUSION: Myeloid cell-derived PDGF contributes to vascular neotissue formation by regulating macrophage apoptosis, smooth muscle cell proliferation and extracellular matrix deposition.
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