Literature DB >> 28523495

Fresh Frozen Versus Formalin-Fixed Paraffin Embedded for Mass Spectrometry Imaging.

Ekta Patel1.   

Abstract

Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry Imaging (MSI) is fast becoming an industry leading technique as a means of investigating the distributions of protein and peptide molecules directly from sections of tissue. Developing protocols for the analysis of FFPE tissue opens up numerous opportunities for novel biomarker discovery, due to the large number of tissue banks containing FFPE biopsy samples. This chapter reports the analysis of both fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues using such protocols.

Entities:  

Keywords:  Formalin-fixed paraffin embedded (FFPE); Fresh frozen; MALDI-MSI; Peptides; Trypsin

Mesh：

Substances：

Year:  2017        PMID： 28523495     DOI： 10.1007/978-1-4939-7051-3_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Complementary neuropeptide detection in crustacean brain by mass spectrometry imaging using formalin and alternative aqueous tissue washes.

Authors:  Nhu Q Vu; Amanda R Buchberger; Jillian Johnson; Lingjun Li
Journal:  Anal Bioanal Chem       Date:  2021-01-05       Impact factor: 4.142

2.  Tissue fixation effects on human retinal lipid analysis by MALDI imaging and LC-MS/MS technologies.

Authors:  Ankita Kotnala; David M G Anderson; Nathan Heath Patterson; Lee S Cantrell; Jeffrey D Messinger; Christine A Curcio; Kevin L Schey
Journal:  J Mass Spectrom       Date:  2021-12       Impact factor: 2.394

Review 3.  Novel methods in adrenal research: a metabolomics approach.

Authors:  Thomas G Papathomas; Na Sun; Vasileios Chortis; Angela E Taylor; Wiebke Arlt; Susan Richter; Graeme Eisenhofer; Gerard Ruiz-Babot; Leonardo Guasti; Axel Karl Walch
Journal:  Histochem Cell Biol       Date:  2019-02-06       Impact factor: 4.304

4.  An optimized MALDI MSI protocol for spatial detection of tryptic peptides in fresh frozen prostate tissue.

Authors:  Therese S Høiem; Maria K Andersen; Marta Martin-Lorenzo; Rémi Longuespée; Britt S R Claes; Anna Nordborg; Frédéric Dewez; Benjamin Balluff; Marco Giampà; Animesh Sharma; Lars Hagen; Ron M A Heeren; Tone F Bathen; Guro F Giskeødegård; Sebastian Krossa; May-Britt Tessem
Journal:  Proteomics       Date:  2022-03-03       Impact factor: 5.393

5.  Colocalization Features for Classification of Tumors Using Desorption Electrospray Ionization Mass Spectrometry Imaging.

Authors:  Paolo Inglese; Gonçalo Correia; Pamela Pruski; Robert C Glen; Zoltan Takats
Journal:  Anal Chem       Date:  2019-05-01       Impact factor: 6.986

6.  Impact of Skin Tissue Collection Method on Downstream MALDI-Imaging.

Authors:  Manoj Yadav; Prem Prashant Chaudhary; Brandon N D'Souza; Jacquelyn Spathies; Ian A Myles
Journal:  Metabolites       Date:  2022-05-30

Review 7.  Matrix-Assisted Laser Desorption/Ionisation Mass Spectrometry Imaging in the Study of Gastric Cancer: A Mini Review.

Authors:  Andrew Smith; Isabella Piga; Manuel Galli; Martina Stella; Vanna Denti; Marina Del Puppo; Fulvio Magni
Journal:  Int J Mol Sci       Date:  2017-12-01       Impact factor: 5.923
  7 in total

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