| Literature DB >> 28522389 |
Xiaoyun Dong1, Yingli Jin2, Di Ming3, Bangbang Li1, Haisi Dong3, Lin Wang4, Tiedong Wang5, Dacheng Wang6.
Abstract
The understanding of the genetic mechanism of Staphylococcus aureus requires efficient tools, however, genetic manipulation in S. aureus is always laborious and time-consuming. Here we proposed a novel CRISPR/dCas9 interference method for the rapid knockdown of target genes. Furthermore, multiple genes can be repressed simultaneously by using this method.Entities:
Keywords: CRISPR interference; Gene knockdown; Staphylococcus aureus
Mesh:
Year: 2017 PMID: 28522389 DOI: 10.1016/j.mimet.2017.05.008
Source DB: PubMed Journal: J Microbiol Methods ISSN: 0167-7012 Impact factor: 2.363