| Literature DB >> 2852219 |
A J Yates1, J F Collins, P J Elder, R E Stephens.
Abstract
Sialyltransferase-1 activity was studied in cultured 12-18 human glioma cells. The apparent Km and Vmax with variable LacCer concentrations were 32 microM and 197 pmoles/mg protein/hr and with variable CMP-NeuAc concentrations were 172 microM and 877 pmoles/mg protein/hr., respectively. The pH optimum towards exogenous LacCer was 6.0 and towards endogenous acceptors was 6.2. The optimum protein:detergent ratio was 1:1. Human beta interferon (1000 units/ml medium) increased sialyltransferase-1 activity only slightly on a protein basis but increased it 47% on a per cell basis. These results demonstrate that one of the biochemical effects of beta-interferon on 12-18 human glioma cells is to stimulate ganglioside synthesis.Entities:
Mesh：
Substances：
Year: 1988 PMID： 2852219 DOI： 10.1007/bf02327394
Source DB: PubMed Journal: J Neurooncol ISSN： 0167-594X Impact factor: 4.130