N Nayan1, A S M Sonnenberg2, W H Hendriks1, J W Cone1. 1. Animal Nutrition Group, Wageningen University, Wageningen, The Netherlands. 2. Plant Breeding, Wageningen University and Research, Wageningen, The Netherlands.
Abstract
AIM: This study evaluated differences between two strains of Ceriporiopsis subvermispora on improving the nutritive value and in vitro degradability of wheat straw. METHODS AND RESULTS: Wheat straw was treated with the fungi for 7 weeks. Weekly samples were analysed for ergosterol content, in vitro gas production (IVGP), chemical composition and lignin-degrading enzyme activity. Ergosterol data showed CS1 to have a faster initial growth than CS2 and reaching a stationary phase after 3 weeks. The IVGP of CS1-treated wheat straw exceeded the control earlier than CS2 (4 vs 5 weeks). CS1 showed a significantly higher (P < 0·001) selectivity in lignin degradation compared to CS2. Both strains showed peak activity of laccase and manganese peroxidase (MnP) at week 1. CS1 showed a significantly higher (P < 0·001) laccase activity, but lower (P = 0·008) MnP activity compared to CS2. CONCLUSION: Both CS strains improved the nutritive value of wheat straw. Variation between strains was clearly demonstrated by their growth pattern and enzyme activities. SIGNIFICANCE AND IMPACT OF THE STUDY: The differences among the two strains provide an opportunity for future selection and breeding programs in improving the extent and selectivity of lignin degradation in agricultural biomass.
AIM: This study evaluated differences between two strains of Ceriporiopsis subvermispora on improving the nutritive value and in vitro degradability of wheat straw. METHODS AND RESULTS:Wheat straw was treated with the fungi for 7 weeks. Weekly samples were analysed for ergosterol content, in vitro gas production (IVGP), chemical composition and lignin-degrading enzyme activity. Ergosterol data showed CS1 to have a faster initial growth than CS2 and reaching a stationary phase after 3 weeks. The IVGP of CS1-treated wheat straw exceeded the control earlier than CS2 (4 vs 5 weeks). CS1 showed a significantly higher (P < 0·001) selectivity in lignin degradation compared to CS2. Both strains showed peak activity of laccase and manganese peroxidase (MnP) at week 1. CS1 showed a significantly higher (P < 0·001) laccase activity, but lower (P = 0·008) MnP activity compared to CS2. CONCLUSION: Both CS strains improved the nutritive value of wheat straw. Variation between strains was clearly demonstrated by their growth pattern and enzyme activities. SIGNIFICANCE AND IMPACT OF THE STUDY: The differences among the two strains provide an opportunity for future selection and breeding programs in improving the extent and selectivity of lignin degradation in agricultural biomass.
Authors: Gijs van Erven; Nazri Nayan; Anton S M Sonnenberg; Wouter H Hendriks; John W Cone; Mirjam A Kabel Journal: Biotechnol Biofuels Date: 2018-09-26 Impact factor: 6.040
Authors: Nazri Nayan; Gijs van Erven; Mirjam A Kabel; Anton Sm Sonnenberg; Wouter H Hendriks; John W Cone Journal: J Sci Food Agric Date: 2019-03-13 Impact factor: 3.638
Authors: Gijs van Erven; Jianli Wang; Peicheng Sun; Pieter de Waard; Jacinta van der Putten; Guus E Frissen; Richard J A Gosselink; Grigory Zinovyev; Antje Potthast; Willem J H van Berkel; Mirjam A Kabel Journal: ACS Sustain Chem Eng Date: 2019-11-05 Impact factor: 8.198