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Literature DB >> 28516120

Aβ Extraction from Murine Brain Homogenates.

Abstract

This protocol details beta-amyloid (Aβ) extraction from transgenic murine brain homogenates. Specifically, mechanical homogenization of brain tissue and sequential extraction of both soluble and insoluble proteins are detailed. DEA extracts soluble proteins, such as Aβ isoforms and APP. Formic acid enables extraction of insoluble protein aggregates, such as Aβ isoforms associated with plaques. This procedure produces soluble and insoluble extracts that are amenable to analysis of Aβ species via western blotting and/or enzyme-linked immunosorbent assays (ELISAs), and these results help assess amyloidogenic burden in animals.

Entities: Chemical

Year: 2016 PMID： 28516120 PMCID： PMC5431584 DOI： 10.21769/BioProtoc.1787

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

3 in total

1. Calpastatin modulates APP processing in the brains of β-amyloid depositing but not wild-type mice.

Authors: Jose Morales-Corraliza; Jason D Berger; Matthew J Mazzella; Thomas A Neubert; Jorge Ghiso; Mala V Rao; Matthias Staufenbiel; Ralph A Nixon; Paul M Mathews
Journal: Neurobiol Aging Date: 2011-12-27 Impact factor: 4.673

2. Omega-3 Fatty Acids Augment the Actions of Nuclear Receptor Agonists in a Mouse Model of Alzheimer's Disease.

Authors: Brad T Casali; Angela W Corona; Monica M Mariani; J Colleen Karlo; Kaushik Ghosal; Gary E Landreth
Journal: J Neurosci Date: 2015-06-17 Impact factor: 6.167

3. ApoE-directed therapeutics rapidly clear β-amyloid and reverse deficits in AD mouse models.

Authors: Paige E Cramer; John R Cirrito; Daniel W Wesson; C Y Daniel Lee; J Colleen Karlo; Adriana E Zinn; Brad T Casali; Jessica L Restivo; Whitney D Goebel; Michael J James; Kurt R Brunden; Donald A Wilson; Gary E Landreth
Journal: Science Date: 2012-02-09 Impact factor: 47.728

3 in total

12 in total

1. Neuronal Protein Tyrosine Phosphatase 1B Hastens Amyloid β-Associated Alzheimer's Disease in Mice.

Authors: Konrad M Ricke; Shelly A Cruz; Zhaohong Qin; Kaveh Farrokhi; Fariba Sharmin; Li Zhang; Michael A Zasloff; Alexandre F R Stewart; Hsiao-Huei Chen
Journal: J Neurosci Date: 2020-01-08 Impact factor: 6.167

2. Cholesterol-metabolizing enzyme cytochrome P450 46A1 as a pharmacologic target for Alzheimer's disease.

Authors: Natalia Mast; Aicha Saadane; Ana Valencia-Olvera; James Constans; Erin Maxfield; Hiroyuki Arakawa; Young Li; Gary Landreth; Irina A Pikuleva
Journal: Neuropharmacology Date: 2017-06-24 Impact factor: 5.250

3. Microglia depletion rapidly and reversibly alters amyloid pathology by modification of plaque compaction and morphologies.

Authors: Brad T Casali; Kathryn P MacPherson; Erin G Reed-Geaghan; Gary E Landreth
Journal: Neurobiol Dis Date: 2020-05-30 Impact factor: 5.996

4. ApoE4 activates C/EBPβ/δ-secretase with 27-hydroxycholesterol, driving the pathogenesis of Alzheimer's disease.

Authors: Zhi-Hao Wang; Yiyuan Xia; Pai Liu; Xia Liu; Laura Edgington-Mitchell; Kecheng Lei; Shan Ping Yu; Xiao-Chuan Wang; Keqiang Ye
Journal: Prog Neurobiol Date: 2021-03-11 Impact factor: 10.885

5. Nuclear receptor agonist-driven modification of inflammation and amyloid pathology enhances and sustains cognitive improvements in a mouse model of Alzheimer's disease.

Authors: Brad T Casali; Erin G Reed-Geaghan; Gary E Landreth
Journal: J Neuroinflammation Date: 2018-02-15 Impact factor: 8.322

6. The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease.

Authors: Paul J Cheng-Hathaway; Erin G Reed-Geaghan; Taylor R Jay; Brad T Casali; Shane M Bemiller; Shweta S Puntambekar; Victoria E von Saucken; Roxanne Y Williams; J Colleen Karlo; Miguel Moutinho; Guixiang Xu; Richard M Ransohoff; Bruce T Lamb; Gary E Landreth
Journal: Mol Neurodegener Date: 2018-06-01 Impact factor: 14.195

7. SPON1 Can Reduce Amyloid Beta and Reverse Cognitive Impairment and Memory Dysfunction in Alzheimer's Disease Mouse Model.

Authors: Soo Yong Park; Joo Yeong Kang; Taehee Lee; Donggyu Nam; Chang-Jin Jeon; Jeong Beom Kim
Journal: Cells Date: 2020-05-21 Impact factor: 6.600

8. Amyloid-beta impairs insulin signaling by accelerating autophagy-lysosomal degradation of LRP-1 and IR-β in blood-brain barrier endothelial cells in vitro and in 3XTg-AD mice.

Authors: Chaitanya Chakravarthi Gali; Elham Fanaee-Danesh; Martina Zandl-Lang; Nicole Maria Albrecher; Carmen Tam-Amersdorfer; Anika Stracke; Vinay Sachdev; Florian Reichmann; Yidan Sun; Afrim Avdili; Marielies Reiter; Dagmar Kratky; Peter Holzer; Achim Lass; Karunya K Kandimalla; Ute Panzenboeck
Journal: Mol Cell Neurosci Date: 2019-07-02 Impact factor: 4.314

9. Leucine Carboxyl Methyltransferase 1 Overexpression Protects Against Cognitive and Electrophysiological Impairments in Tg2576 APP Transgenic Mice.

Authors: Madhumathi Gnanaprakash; Agnieszka Staniszewski; Hong Zhang; Rose Pitstick; Michael P Kavanaugh; Ottavio Arancio; Russell E Nicholls
Journal: J Alzheimers Dis Date: 2021 Impact factor: 4.472

10. GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer's disease.

Authors: Peng Xu; Jerry C Chang; Xiaopu Zhou; Wei Wang; Michael Bamkole; Eitan Wong; Karima Bettayeb; Lu-Lin Jiang; Timothy Huang; Wenjie Luo; Huaxi Xu; Angus C Nairn; Marc Flajolet; Nancy Y Ip; Yue-Ming Li; Paul Greengard
Journal: J Exp Med Date: 2021-06-22 Impact factor: 17.579