Literature DB >> 2851490

Two new tools: multi-purpose cloning vectors that carry kanamycin or spectinomycin/streptomycin resistance markers.

J A Kirschman1, J H Cramer.   

Abstract

We have constructed two new multi-purpose cloning vectors, pJKKmf(-) and pJKSp/Smf(-), that carry resistance to kanamycin (Km) and spectinomycin/streptomycin (Sp/Sm), respectively. These plasmids are based on pGEM3Zf(-) and therefore contain a pUC-vector-derived multiple cloning site for 13 restriction sites flanked by T7 and SP6 RNA polymerase promoters, the alpha-peptide coding region of beta-galactosidase for blue/white colony screening and the bacteriophage f1 origin of replication for production of single-stranded DNA in the presence of a helper phage. We have used these vectors to reclone sequences from a maize genomic library, to produce radiolabeled RNA probes and to make single-stranded DNA.

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Year:  1988        PMID： 2851490     DOI： 10.1016/0378-1119(88)90609-9

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  13 in total

1.  Nuclear scaffolds and scaffold-attachment regions in higher plants.

Authors:  G Hall; G C Allen; D S Loer; W F Thompson; S Spiker
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-15       Impact factor: 11.205

2.  Identification of a novel allele of SIR3 defective in the maintenance, but not the establishment, of silencing in Saccharomyces cerevisiae.

Authors:  S Enomoto; S D Johnston; J Berman
Journal:  Genetics       Date:  2000-06       Impact factor: 4.562

3.  Chlamydomonas telomere sequences are A+T-rich but contain three consecutive G-C base pairs.

Authors:  M E Petracek; P A Lefebvre; C D Silflow; J Berman
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

4.  Large-scale production of SAGE libraries from microdissected tissues, flow-sorted cells, and cell lines.

Authors:  Jaswinder Khattra; Allen D Delaney; Yongjun Zhao; Asim Siddiqui; Jennifer Asano; Helen McDonald; Pawan Pandoh; Noreen Dhalla; Anna-Liisa Prabhu; Kevin Ma; Stephanie Lee; Adrian Ally; Angela Tam; Danne Sa; Sean Rogers; David Charest; Jeff Stott; Scott Zuyderduyn; Richard Varhol; Connie Eaves; Steven Jones; Robert Holt; Martin Hirst; Pamela A Hoodless; Marco A Marra
Journal:  Genome Res       Date:  2006-11-29       Impact factor: 9.043

5.  Hairpin RNAs derived from RNA polymerase II and polymerase III promoter-directed transgenes are processed differently in plants.

Authors:  Ming-Bo Wang; Christopher A Helliwell; Li-Min Wu; Peter M Waterhouse; W James Peacock; Elizabeth S Dennis
Journal:  RNA       Date:  2008-03-26       Impact factor: 4.942

6.  A positive screen for cloning PCR products.

Authors:  P Keese; L Graf
Journal:  Nucleic Acids Res       Date:  1996-09-01       Impact factor: 16.971

7.  Novel embryonic regulation of Ca(2+)-activated K+ channel expression in Drosophila.

Authors:  T Thomas; B Wang; R Brenner; N S Atkinson
Journal:  Invert Neurosci       Date:  1997-03

8.  The structure and expression of the wheat starch synthase III gene. Motifs in the expressed gene define the lineage of the starch synthase III gene family.

Authors:  Z Li; G Mouille; B Kosar-Hashemi; S Rahman; B Clarke; K R Gale; R Appels; M K Morell
Journal:  Plant Physiol       Date:  2000-06       Impact factor: 8.340

9.  RecOR suppression of recF mutant phenotypes in Escherichia coli K-12.

Authors:  S J Sandler; A J Clark
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

10.  Direct genetic selection of a maize cDNA for dihydrodipicolinate synthase in an Escherichia coli dapA- auxotroph.

Authors:  D A Frisch; A M Tommey; B G Gengenbach; D A Somers
Journal:  Mol Gen Genet       Date:  1991-08
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