Patricia Altea-Manzano1,2, Juan Diego Unciti-Broceta2, Victoria Cano-Cortes1,3, María Paz Ruiz-Blas1,3, Teresa Valero-Griñan1,3, Juan Jose Diaz-Mochon1,3, Rosario Sanchez-Martin1,3. 1. GENYO:Pfizer - Universidad de Granada-Junta de Andalucía Centre for Genomics & Oncological Research, Health Science Technological Park (PTS), Avenida de la Ilustración 114, 18016 Granada, Spain. 2. R&D Deparment, NanoGetic S. L. Granada HealthScienceTechnological Park (PTS), Avenida de la Innovación 1, Edificio BIC, 18016 Granada, Spain. 3. Department of Medicinal & Organic Chemistry, University of Granada, Campus de Cartuja s/n, 18071 Granada, Spain.
Abstract
AIM: To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. MATERIAL & METHODS: Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. RESULTS: The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. CONCLUSION: A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed. [Formula: see text].
AIM: To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. MATERIAL & METHODS: Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. RESULTS: The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. CONCLUSION: A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed. [Formula: see text].
Authors: José M Espejo-Román; Belén Rubio-Ruiz; Victoria Cano-Cortés; Olga Cruz-López; Saúl Gonzalez-Resines; Carmen Domene; Ana Conejo-García; Rosario M Sánchez-Martín Journal: Pharmaceutics Date: 2022-04-04 Impact factor: 6.525
Authors: Agustín Robles-Remacho; M Angélica Luque-González; Roberto A González-Casín; M Victoria Cano-Cortés; F Javier Lopez-Delgado; Juan J Guardia-Monteagudo; Mario Antonio Fara; Rosario M Sánchez-Martín; Juan José Díaz-Mochón Journal: Talanta Date: 2021-01-09 Impact factor: 6.057
Authors: Antonio Delgado-Gonzalez; Jose Antonio Laz-Ruiz; M Victoria Cano-Cortes; Ying-Wen Huang; Veronica D Gonzalez; Juan Jose Diaz-Mochon; Wendy J Fantl; Rosario M Sanchez-Martin Journal: Anal Chem Date: 2022-07-22 Impact factor: 8.008