Literature DB >> 2851199

Alkaline and acid phosphatase demonstration in human bone and cartilage: effects of fixation interval and methacrylate embedments.

H E Gruber1, G J Marshall, L M Nolasco, M E Kirchen, D L Rimoin.   

Abstract

Human bone and cartilage specimens were evaluated for acid and alkaline phosphatase localization following varying fixation periods for fresh or frozen tissue. Formalin fixations of up to 183 hr were followed by embedment in methyl methacrylate; frozen tissue was examined either without fixation or following fixation for up to 1 hr and subsequent glycol or methyl methacrylate embedding. The humeral epiphysis of a young patient with osteogenic sarcoma showed optimum acid and alkaline phosphatase localization following fixation for periods up to 15 hr and embedding in methyl methacrylate. Frozen costochondral junction from a newborn with osteogenesis imperfecta type II showed optimum acid and alkaline phosphatase localization following 30 min fixation in formalin and embedding in methyl methacrylate or after 5 min fixation and embedding in glycol methacrylate.

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Year:  1988        PMID： 2851199     DOI： 10.3109/10520298809107604

Source DB:  PubMed          Journal:  Stain Technol        ISSN： 0038-9153


  10 in total

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Authors:  Xuezhong Qin; Jon E Wergedal; Mark Rehage; Kiet Tran; Jacqueline Newton; Paggie Lam; David J Baylink; Subburaman Mohan
Journal:  Endocrinology       Date:  2006-08-31       Impact factor: 4.736

2.  Quantitative histology of cartilage vascular canals in the human rib. Findings in normal neonates and children and in achondrogenesis II-hypochondrogenesis.

Authors:  H E Gruber; R S Lachman; D L Rimoin
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3.  The Effects of Storage Conditions on the Preservation of Enzymatic Activity in Bone.

Authors:  Christi N Cosby; Nancy W Troiano; Melissa A Kacena
Journal:  J Histotechnol       Date:  2008-12       Impact factor: 0.714

4.  Treatment with SiMiaoFang, an anti-arthritis chinese herbal formula, inhibits cartilage matrix degradation in osteoarthritis rat model.

Authors:  Ying Xu; Qian Liu; Zhen-Li Liu; Li Lim; Wei-Heng Chen; Na Lin
Journal:  Rejuvenation Res       Date:  2013-10       Impact factor: 4.663

5.  Lack of effect of ovariectomy on divalent cation regulation of skeletal adenylate cyclase.

Authors:  S B Oldham; H E Gruber; R K Rude
Journal:  Osteoporos Int       Date:  1992-07       Impact factor: 4.507

6.  Reduction of dietary magnesium by only 50% in the rat disrupts bone and mineral metabolism.

Authors:  R K Rude; H E Gruber; H J Norton; L Y Wei; A Frausto; J Kilburn
Journal:  Osteoporos Int       Date:  2006-04-07       Impact factor: 4.507

Review 7.  A Baseline for Skeletal Investigations in Medaka (Oryzias latipes): The Effects of Rearing Density on the Postcranial Phenotype.

Authors:  Claudia Di Biagio; Zachary Dellacqua; Arianna Martini; Ann Huysseune; Michele Scardi; Paul Eckhard Witten; Clara Boglione
Journal:  Front Endocrinol (Lausanne)       Date:  2022-06-30       Impact factor: 6.055

8.  Growth hormone involvement in the regulation of tartrate-resistant acid phosphatase-positive cells that are active in cartilage and bone resorption.

Authors:  D Lewinson; P Shenzer; Z Hochberg
Journal:  Calcif Tissue Int       Date:  1993-03       Impact factor: 4.333

9.  Sanmiao formula inhibits chondrocyte apoptosis and cartilage matrix degradation in a rat model of osteoarthritis.

Authors:  Ying Xu; Guo-Jing Dai; Qian Liu; Zhen-Li Liu; Zhi-Qian Song; Li Li; Wei-Heng Chen; Na Lin
Journal:  Exp Ther Med       Date:  2014-07-24       Impact factor: 2.447

10.  The calcitonin-like system is an ancient regulatory system of biomineralization.

Authors:  João C R Cardoso; Rute C Félix; Vinícius Ferreira; MaoXiao Peng; Xushuai Zhang; Deborah M Power
Journal:  Sci Rep       Date:  2020-05-05       Impact factor: 4.379
  10 in total

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