Farzaneh Salarnia1, Sima Besharat2, Sare Zhand3, Naeme Javid4, Behnaz Khodabakhshi5, Abdolvahab Moradi6. 1. Researcher, Infectious Disease Research Center, Golestan University of Medical Sciences, Gorgan, Golestan, Iran. 2. Assistant Professor, PhD, Golestan Research Center of Gastroentrology and Hepatology, Golestan University of Medical Sciences, Gorgan, Golestan, Iran. 3. PhD Candidate, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Golestan, Iran. 4. Researcher, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Golestan, Iran. 5. Associate Professor, Golestan Research Center of Gastroentrology and Hepatology, Golestan University of Medical Sciences, Gorgan, Golestan, Iran. 6. Professor, Golestan Research Center of Gastroentrology and Hepatology, Golestan University of Medical Sciences, Gorgan, Golestan, Iran.
Abstract
INTRODUCTION: Specific mutations in Hepatitis-B Virus (HBV) genome would proceed the development of chronic hepatitis B to more serious consequences like cirrhosis and end-stage liver disease. AIM: This study was designed to detect deletion and insertion mutational patterns in the X-gene region in a population of chronic HBV and related cirrhosis patients. MATERIALS AND METHODS: Sixty eight chronic HBV patients and 34 HBV-related cirrhotics were recruited from the eligible cases (N=50) referred to the academic hospitals of Gorgan city, Northeast of Iran, between Jan 2011 to Dec 2013. The HBx region was amplified by semi-nested PCR using serum samples and analyzed by sequencing. RESULTS: Our findings showed deletions and insertions in the C-terminal of HBx of the cirrhotic group and 8 bp found in two chronic HBV cases (2.9%). We detected 15 types of deletions in cirrhotic cases such as 1762-1768, 1763-1770, 1769-1773 and T1771/A1775. CONCLUSION: We found that the frequencies of deletion and insertion mutations in C-terminal of X-gene were more seen in cirrhotic patients comparing to chronic HBV cases in our area of study.
INTRODUCTION: Specific mutations in Hepatitis-B Virus (HBV) genome would proceed the development of chronic hepatitis B to more serious consequences like cirrhosis and end-stage liver disease. AIM: This study was designed to detect deletion and insertion mutational patterns in the X-gene region in a population of chronic HBV and related cirrhosispatients. MATERIALS AND METHODS: Sixty eight chronic HBVpatients and 34 HBV-related cirrhotics were recruited from the eligible cases (N=50) referred to the academic hospitals of Gorgan city, Northeast of Iran, between Jan 2011 to Dec 2013. The HBx region was amplified by semi-nested PCR using serum samples and analyzed by sequencing. RESULTS: Our findings showed deletions and insertions in the C-terminal of HBx of the cirrhotic group and 8 bp found in two chronic HBV cases (2.9%). We detected 15 types of deletions in cirrhotic cases such as 1762-1768, 1763-1770, 1769-1773 and T1771/A1775. CONCLUSION: We found that the frequencies of deletion and insertion mutations in C-terminal of X-gene were more seen in cirrhotic patients comparing to chronic HBV cases in our area of study.
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Authors: Carolina González; David Tabernero; Maria Francesca Cortese; Josep Gregori; Rosario Casillas; Mar Riveiro-Barciela; Cristina Godoy; Sara Sopena; Ariadna Rando; Marçal Yll; Rosa Lopez-Martinez; Josep Quer; Rafael Esteban; Maria Buti; Francisco Rodríguez-Frías Journal: World J Gastroenterol Date: 2018-05-21 Impact factor: 5.742